Production of single chain Fab (scFab) fragments in Bacillus megaterium

Microbial Cell Factories

Volumn 6, Issue , 2007, Pages

  Jordan, Eva ^a   Al Halabi, Laila ^a   Schirrmann, Thomas ^a   Hust, Michael ^a   Dübel, Stefan ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

C REACTIVE PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; LYSOZYME; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BACILLUS MEGATERIUM; BACTERIUM CULTURE; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GRAM POSITIVE BACTERIUM; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; SUPERNATANT;

BACILLUS MEGATERIUM; ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA;

EID: 38649097518     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-6-38     Document Type: Article

References (31)

1. Dübel S: Handbook of therapeutic antibodies Weinheim: Willey-VCH 2007
2. Hust M, Dübel S: Mating antibody phage display to proteomics. Trends Biotechnol 2004, 22:8-14. 10.1016/j.tibtech.2003.10.011 14690617
3. Taussig MJ, Stoevesandt O, Borrebaeck C, Bradbury A, Dübel S, Frank R, Gibson T, Gold L, Herberg F, Hermjakob H, Hoheisel J, Joos T, Konthur Z, Landegren U, Plückthun A, Ueffing M, Uhlen M: ProteomeBinders: Planning a European Resource of Affinity Reagents for Analysis of the Human Proteome. Nature Methods 2007, 4:13-17. 10.1038/nmeth0107-13 17195019
4. Bird RE, Hardman KD, Jacobsen JW, Johnson S, Kaufman BM, Lee SM, Lee T, Pope SH, Riordan GS, Whitlow M: Single-chain antigen-binding proteins. Science 1988, 242:423-426. 10.1126/science.3140379 3140379
5. Huston JS, Levinson D, Mudgett HM, Tai MS, Novotny J, Margolies MN, Ridge RJ, Bruccoloreri RE, Haber E, Crea R, Oppermann H: Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digosin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA 1988, 85:5879-5883. 281868 3045807 10.1073/ pnas.85.16.5879
6. Marks JD, Hoogenboom HR, Griffiths AD, Winter G: Molecular evolution of proteins on filamentous phage. J Biol Chem 1992, 267:16007-16010. 1644788
7. Bird RE, Walker BW: Single chain variable regions. Trends Biotech 1991, 9:132-137. 10.1016/0167-7799(91)90044-I
8. Iliades P, Dougan DA, Oddie GW, Metzger DW, Hudson PJ, Kortt AA: Single-chain Fv of anti-idiotype 11-1G10 antibody interacts with antibody NC41 single-chain Fv with a higher affinity than the affinity for the interaction of the parent Fab fragments. J Protein Chem 1998, 17:245-254. 10.1023/A:1022584702108 9588948
9. Skerra A, Pfitzinger I, Plückthun A: The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique. Bio/Technology 1993, 9:273-278. 10.1038/nbt0391-273
10. Nachman RL, Engle RL Jr, Stein S: Subtypes of Bence-Jones proteins. J Immunlogy 1965, 9(2):295-299.
11. Kramer K, Fiedler M, Skerra A, Hock B: A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of Fab fragments and leads to improved recombinant immunoglobulin stability. Biosensors & Bioelectronics 2002, 17:305-313. 10.1016/S0956-5663(01)00292-5 11849927
12. Hust M, Jostock T, Menzel C, Voedisch B, Mohr A, Brenneis M, Kirsch MI, Meier D, Dübel S: Single chain (scFab) fragment. BMC-Biotechnology 2007, 7:14 1829395 17346344 10.1186/1472-6750-7-14
13. Inoue Y, Ohta T, Tada H, Iwasa S, Udaka S, Yamagata H: Efficient production of a functional mouse/human chimeric Fab' against human urokinase-type plasminogen activator by Bacillus brevis. Appl Microbiol Biotechnol 1997, 48:487-492. 10.1007/s002530051084 9390457
14. Shiroza T, Shinozaki-Kuwahara N, Hayakawa M, Shibata Y, Hashizume T, Udaka S, Abiko Y: Production of a single-chain variable fraction capable of inhibiting the Streptococcus mutans glucosyltransferase in Bacillus brevis: Construction of a chimeric shuttle plasmid secreting its gene product. BBA 2003, 1626:57-64. 12697330
15. Wu SC, Ye R, Wu XC, Ng SC, Wong SL: Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperons. J Bacteriol 1998, 180:2830-2835. 107245 9603868
16. Wu SC, Yeung JC, Duan Y, Ye R, Szarka SJ, Habibi HR, Wong SL: Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: Effects of molecular chaperones and a wall-bound protease on antibody fragment production. Appl Env Microbiol 2002, 68:3261-3269. 10.1128/AEM.68.7.3261-3269.2002
17. Vary PS: Prime time for Bacillus megaterium. Microbiology 1994, 140:1001-1013. 8025666
18. Jordan E, Hust M, Roth A, Biedendieck R, Schirrmann T, Jahn D, Dübel S: Production of recombinant antibody fragments in Bacillus megaterium. Microbial Cell Factories 2007, 6:2. 1797049 17224052 10.1186/ 1475-2859-6-2
19. Quintero-Hernandez V, Juarez-Gonzalez VR, Ortiz-Leon M, Sanchez R, Possani LD, Becerril B: The change of the scFv into the Fab format improves the stability and in vivo toxin neutralization capacity of recombinant antibodies. Mol Immunol 2007, 44:1307-15. 10.1016/ j.molimm.2006.05.009 16814388
20. Chen C, Snedecor B, Nishihara JC, Joly JC, McFarland N, Andersen DC, Battersby JE, Champion KM: High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain. Biotechnol Bioeng 2004, 85:463-74. 10.1002/bit.20014 14760686
21. Ward ES, Güssow D, Griffiths AD, Jones PT, Winter G: Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 1989, 341:544-546. 10.1038/ 341544a0 2677748
22. Lauer B, Ottleben I, Jacobsen HJ, Reinard T: Production of a Single-Chain Variable Fragment Antibody against Fumonisin B1. J Agric Food Chem 2005, 53:899-904. 10.1021/jf048651s 15712995
23. Schenk JA, Sellrie F, Böttger V, Menning A, Stöcklein WF, Micheel B: Generation and application of a fluorescein-specific single chain antibody. Biochimie 2007, 89:1304-1311. 10.1016/ j.biochi.2007.06.008 17686564
24. Graumann K, Premstaller A: Manufacturing of recombinant therapeutic proteins in microbial systems. Biotechnol J 2006, 1:164-186. 10.1002/ biot.200500051 16892246
25. Skerra A, Plückthun A: Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 1988, 240:1038-1041. 10.1126/ science.3285470 3285470
26. Sindhu I, Chhibber S, Capalash N, Sharma P: Production of cellulase-free xylanase from Bacillus megaterium by solid state fermentation for biobleaching of pulp. Curr Microbiol 2006, 53:167-172. 10.1007/ s00284-006-0051-4 16832724
27. Wittchen KD, Meinhardt F: Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl Microbiol Biotechnol 1995, 42:871-877. 10.1007/s002530050345 7766087
28. Barg H, Malten M, Jahn M, Jahn D: Protein and vitamin production in Bacillus megaterium. Microbial Processes and Products Totowa: Humana press Barredo JL 2005, 18:205-224. [Methods in Biotechnology]
29. Sambrook J Russell DW (Eds) Molecular cloning: A laboratory manual New York: Cold Spring Harbor Laboratory Press 3 2001.
30. Schmiedl A, Breitling F, Dübel S: Expression of a bispecific dsFv-dsFv' antibody fragment in Escherichia coli. Protein Engineering 2000, 13:725-734. 10.1093/protein/13.10.725 11112512
31. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0 5432063