Combined localization and real-time functional studies using a GFP-tagged ABCG2 multidrug transporter

Biochemical and Biophysical Research Communications

Volumn 367, Issue 3, 2008, Pages 667-673

  Orbán, Tamás I ^a   Seres, László ^a   Özvegy Laczka, Csilla ^a   Elkind, N Barry ^a   Sarkadi, Balázs ^a   Homolya, László ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

ABCG2; Dye transport; Functional assay; Green fluorescent protein; Hoechst 33342; Multidrug resistance

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BREAST CANCER RESISTANCE PROTEIN; EPITOPE; GLYCOPROTEIN P; GREEN FLUORESCENT PROTEIN; HOE 33342; MEMBRANE ANTIGEN; METHOTREXATE;

ARTICLE; CELL FUNCTION; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DIMERIZATION; ENZYME ACTIVITY; GLYCOSYLATION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATASES; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BENZIMIDAZOLES; CELL LINE; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; FLOW CYTOMETRY; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HUMANS; KIDNEY; NEOPLASM PROTEINS; RECOMBINANT FUSION PROTEINS; SPODOPTERA;

EID: 38649096279     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.12.172     Document Type: Article

References (23)

1. Allikmets R., Schriml L.M., Hutchinson A., Romano-Spica V., and Dean M. A human placenta-specific ATP-binding cassette gene (ABCP) on chromosome 4q22 that is involved in multidrug resistance. Cancer Res. 58 (1998) 5337-5339
2. Doyle L.A., Yang W., Abruzzo L.V., Krogmann T., Gao Y., Rishi A.K., and Ross D.D. A multidrug resistance transporter from human MCF-7 breast cancer cells. Proc. Natl. Acad. Sci. USA 95 (1998) 15665-15670
3. Miyake K., Mickley L., Litman T., Zhan Z., Robey R., Cristensen B., Brangi M., Greenberger L., Dean M., Fojo T., and Bates S.E. Molecular cloning of cDNAs which are highly overexpressed in mitoxantrone-resistant cells: demonstration of homology to ABC transport genes. Cancer Res. 59 (1999) 8-13
4. Lage H., and Dietel M. Effect of the breast-cancer resistance protein on atypical multidrug resistance. Lancet. Oncol. 1 (2000) 169-175
5. Krishnamurthy P., and Schuetz J.D. Role of ABCG2/BCRP in biology and medicine. Annu. Rev. Pharmacol. Toxicol. 46 (2006) 381-410
6. Sarkadi B., Homolya L., Szakacs G., and Varadi A. Human multidrug resistance ABCB and ABCG transporters: participation in a chemoimmunity defense system. Physiol. Rev. 86 (2006) 1179-1236
7. Zhou S., Schuetz J.D., Bunting K.D., Colapietro A.M., Sampath J., Morris J.J., Lagutina I., Grosveld G.C., Osawa M., Nakauchi H., and Sorrentino B.P. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat. Med. 7 (2001) 1028-1034
8. Stauber R.H., Horie K., Carney P., Hudson E.A., Tarasova N.I., Gaitanaris G.A., and Pavlakis G.N. Development and applications of enhanced green fluorescent protein mutants. Biotechniques 24 (1998) 462-466 468-471
9. Xia C.Q., Milton M.N., and Gan L.S. Evaluation of drug-transporter interactions using in vitro and in vivo models. Curr. Drug Metab. 8 (2007) 341-363
10. Ozvegy C., Varadi A., and Sarkadi B. Characterization of drug transport, ATP hydrolysis, and nucleotide trapping by the human ABCG2 multidrug transporter. Modulation of substrate specificity by a point mutation. J. Biol. Chem. 277 (2002) 47980-47990
11. Ozvegy-Laczka C., Koblos G., Sarkadi B., and Varadi A. Single amino acid (482) variants of the ABCG2 multidrug transporter: major differences in transport capacity and substrate recognition. Biochim. Biophys. Acta. 1668 (2005) 53-63
12. Ozvegy C., Litman T., Szakacs G., Nagy Z., Bates S., Varadi A., and Sarkadi B. Functional characterization of the human multidrug transporter, ABCG2, expressed in insect cells. Biochem. Biophys. Res. Commun. 285 (2001) 111-117
13. Telbisz A., Muller M., Ozvegy-Laczka C., Homolya L., Szente L., Varadi A., and Sarkadi B. Membrane cholesterol selectively modulates the activity of the human ABCG2 multidrug transporter. Biochim. Biophys. Acta. 1768 (2007) 2698-2713
14. Sarkadi B., Price E.M., Boucher R.C., Germann U.A., and Scarborough G.A. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J. Biol. Chem. 267 (1992) 4854-4858
15. Ozvegy-Laczka C., Varady G., Koblos G., Ujhelly O., Cervenak J., Schuetz J.D., Sorrentino B.P., Koomen G.J., Varadi A., Nemet K., and Sarkadi B. Function-dependent conformational changes of the ABCG2 multidrug transporter modify its interaction with a monoclonal antibody on the cell surface. J. Biol. Chem. 280 (2005) 4219-4227
16. Homolya L., Hollo M., Muller M., Mechetner E.B., and Sarkadi B. A new method for a quantitative assessment of P-glycoprotein-related multidrug resistance in tumour cells. Br. J. Cancer 73 (1996) 849-855
17. Takada T., Suzuki H., and Sugiyama Y. Characterization of polarized expression of point- or deletion-mutated human BCRP/ABCG2 in LLC-PK1 cells. Pharm. Res. 22 (2005) 458-464
18. Allen J.D., van Loevezijn A., Lakhai J.M., van der Valk M., van Tellingen O., Reid G., Schellens J.H., Koomen G.J., and Schinkel A.H. Potent and specific inhibition of the breast cancer resistance protein multidrug transporter in vitro and in mouse intestine by a novel analogue of fumitremorgin C. Mol. Cancer Ther. 1 (2002) 417-425
19. Pal A., Mehn D., Molnar E., Gedey S., Meszaros P., Nagy T., Glavinas H., Janaky T., von Richter O., Bathori G., Szente L., and Krajcsi P. Cholesterol potentiates ABCG2 activity in a heterologous expression system: improved in vitro model to study function of human ABCG2. J. Pharmacol. Exp. Ther. 321 (2007) 1085-1094
20. Janvilisri T., Venter H., Shahi S., Reuter G., Balakrishnan L., and van Veen H.W. Sterol transport by the human breast cancer resistance protein (ABCG2) expressed in Lactococcus lactis. J. Biol. Chem. 278 (2003) 20645-20651
21. Abbott B.L., Colapietro A.M., Barnes Y., Marini F., Andreeff M., and Sorrentino B.P. Low levels of ABCG2 expression in adult AML blast samples. Blood 100 (2002) 4594-4601
22. Scharenberg C.W., Harkey M.A., and Torok-Storb B. The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hematopoietic progenitors. Blood 99 (2002) 507-512
23. Kim M., Turnquist H., Jackson J., Sgagias M., Yan Y., Gong M., Dean M., Sharp J.G., and Cowan K. The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. Clin. Cancer Res. 8 (2002) 22-28