A new method for quantitative assessment of P-glycoprotein-related multidrug resistance in tumour cells

British Journal of Cancer

Volumn 73, Issue 7, 1996, Pages 849-855

  Homolya, L ^a   Hollo Z ^a   Muller M ^a   Mechetner, E B ^b   Sarkadi, B ^a  

^a National Institute of Haematology and Immunology Budapest   (Hungary)

^b Ingenex   (United States)

Author keywords

Calcein; Multidrug resistance activity factor; Multidrug resistance in cancer; P glycoprotein

Indexed keywords

CALCEIN; CALCEIN ACETOXY METHYL ESTER; GLYCOPROTEIN P; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOACCUMULATION; CANCER CELL; CELL SURFACE; CELL SURVIVAL; CONTROLLED STUDY; CYTOMETRY; DIAGNOSTIC ACCURACY; FLOW CYTOMETRY; FLUOROMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; MOUSE; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REPRODUCIBILITY; TUMOR CELL LINE;

EID: 0029878923     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/bjc.1996.151     Document Type: Article

References (28)

1. ALTENBERG GA, VANOYE CG, HORTON JK AND REUSS L. (1994). Undirectional fluxes of rhodamine 123 in multidrug-resistant cells: Evidence against direct drug extrusion from the plasma membrane. Proc. Natl Acad. Sci. USA, 91, 4654-4657.
2. BORNMANN WG AND ROEPE DR. (1994). Analysis of drug transport kinetics in multidrug-resistant cells using a novel coumarin-vinblastine compound. Biochemistry, 33, 12665-12675.
3. BRUGGEMANN EP, CURRIER SJ, GOTTESMAN MM AND PASTAN I. (1992). Characterization of the azidopine and vinblastine binding site of P-glycoprotein. J. Biol. Chem., 267, 21020-21026.
4. COLE SPC, BHARDWAY G, GERLACH JH, MACKIE JE, GRANT CE, ALMQUIST KC, STEWART AJ, KURTZ EU, DUNCAN AMV AND DEELEY GG. (1992). Overexpression of a novel transporter gene in a multidrug resistant human lung cancer cell line. Science, 258, 1650-1654.
5. CHAUDHARY PM AND RONINSON IB. (1991). Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells. Cell, 66, 85-94.
6. FELLER N, BROXTERMAN HJ, WÄHRER DCR AND PINEDO HM. (1995a). ATP-dependent efflux of calcein by the multidrug resistance protein (MRP): no inhibition by intracellular glutathione depletion. FEBS Lett., 368, 385-388.
7. FELLER N, KUIPER CM, LANKELMA J, RUHDAL JK, SCHEPER RJ, PINEDO HM AND BROXTERMAN HJ. (1995b). Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometry. Br. J. Cancer, 72, 543-549.
8. FREY T, YUE S, HAUGHLAND RP. (1995). Dyes providing increased sensitivity in flow-cytometric dye-efflux assays for multidrug resistance. Cytometry, 20, 218-227.
9. GERMANN UA, WILLINGHAM MC, PASTAN I AND GOTTESMAN MM. (1990). Expression of the human multidrug transporter in insect cells by a recombinant baculovirus. Biochemistry, 29, 2295-2303.
10. GOLDSTEIN LJ, PASTAN I AND GOTTESMAN MM. (1992). Multidrug resistance in human cancer. Crit. Rev. Oncol. Haematol., 12, 243-253.
11. GOTTESMAN MM AND PASTAN I. (1993). Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu. Rev. Biochem., 62, 385-427.
12. HAUGHLAND RP AND LARISON KD (EDS). (1992). Low molecular weight tracers: fluorescent dyes for assessing vital cell functions. In Handbook of Fluorescent Probes and Research Chemicals 1992-1994, pp. 163, 172-173. Molecular Probes: Eugene, OR.
13. HERWEIJER H, ENGH G AND NOOTER K. (1989). A rapid and sensitive flow cytometric P-glycoprotein method for the detection of multi-drug resistant cells. Cytometry, 10, 463-468.
14. HOLLÓ ZS, HOMOLYA L, DAVIS CW AND SARKADI B (1994). Calcein accumulation as a fluorometric functional assay of the multidrug transporter. Biochim. Biophys. Acta, 1191, 384-388.
15. HOMOLYA L, HOLLÓ ZS, GERMANN UA, PASTAN I, GOTTESMAN MM AND SARKADI B. (1993). Fluorescent cellular indicators are extruded by the multidrug resistance protein. J. Biol. Chem., 268, 21493-21496.
16. LEE JS, PAULL K, ALVAREZ M, HOSE C, MONKS A, GREVER M, FOJO AT AND BATES SE. (1994). Rhodamine efflux patterns predict P-glycoprotein substrates in the National Cancer Institute Drug Screen. Mol. Pharmacol., 46, 627-638.
17. LELONG IH, GUZIKOWSKI AP, HAUGLAND RP, PASTAN I, GOTTESMAN MM AND WILLINGHAM MC. (1991). Fluorescent verapamil derivative for monitoring activity of the multidrug transporter. Mol. Pharmacol., 40, 490-494.
18. MECHETNER EB AND RONINSON IB. (1992). Efficient inhibition of P-glycoprotein mediated multidrug resistance with a monoclonal antibody. Proc. Natl Acad. Sci. USA, 89, 5824-5828.
19. NEYFAKH AA. (1988). Use of fluorescent dyes as molecular probes for the study of multidrug resistance. Exp. Cell Res., 174, 168-174.
20. ROEPE PD. (1992). Analysis of the steady state and initial rate of doxorubicin efflux from a series of multidrug-resistant cells expressing different levels of P-glycoprotein. Biochemistry, 31, 12555-12564.
21. i homeostasis in multidrug-resistant (MDR) cells: further characterization of a series of MDR cell lines expressing different levels of P-glycoprotein. Biochemistry, 32, 11042-11056.
22. SARKADI B, PRICE EM, BOUCHER RC, GERMANN UA AND SCARBOROUGH GA. (1992). Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J. Biol. Chem., 267, 4854-4858.
23. SPOLESTRA EC, WESTERHOFF HV, DEKKER H AND LANKELMA J. (1992). Kinetics of daunorubicin transport by P-glycoprotein of intact cells. Eur. J. Biochem., 207, 567-579.
24. TANAKA S, CURRIER SJ, BRUGGEMANN EP, GERMANN UA, PASTAN I AND GOTTESMAN MM. (1990). Use of recombinant P-glycoprotein fragments to produce antibodies to the multidrug transporter. Biochem. Biophys. Res. Comm., 166, 180-186.
25. THIEBAUT F, CURRIER SJ, WHITAKER J, HAUGHLAND RP, GOTTESMAN MM, PASTAN I AND WILLINGHAM MC. (1990). Activity of the multidrug transporter results in alkalinization of the cytosol: measurement of cytosolic pH by microinjection of a pH-sensitive dye. J. Histochem. Cytochem., 38, 685-690.
26. WALL DM, HU XF, ZALCBERG JR AND PARKIN JD. (1991). Rapid functional assay for multidrug resistance in human tumor cell lines using the fluorescent indicator Fluo-3. J. Natl Cancer Inst., 83, 206-207.
27. WALL DM, SPARROW R, HU XF, NADALIN G, ZALCBERG JR, MARSCHNER IC, VAN DER WEYDEN M AND PARKIN JD. (1993). Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, Fluo-3. Eur. J. Cancer, 29, 1024-1027.
28. WEAVER JL, PINE PS, ASZALOS A, SCHOENLEIN PV, CURRIER SJ, PADMANABHAN R AND GOTTESMAN MM. (1991). Laser scanning and confocal microscopy of daunorubicin, doxorubicin, and rhodamine 123 in multidrug-resistant cells. Exp. Cell Res., 196, 323-329.