Identification of the catalytic nucleophile in Family 42 β-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis

FEBS Letters

Volumn 581, Issue 13, 2007, Pages 2441-2446

  Shaikh, Fathima Aidha ^a   Müllegger, Johannes ^a   He, Shouming ^a   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Bacillus subtilis galactosidases; Catalytic nucleophile; Glycosidase; Glycoside hydrolase; Intermediate trapping; Mechanism based inactivator

Indexed keywords

2,4 DINITROPHENYL 2 DEOXY 2 FLUORO BETA DEXTRO GALACTOPYRANOSIDE; BETA GALACTOSIDASE; GLUTAMYLTHREONYLSERYLPROLYLSERYLTYROSYLSERYLALANYLALANYLSERYLLEUCINE; PEPTIDE; PYRANOSIDE; UNCLASSIFIED DRUG; YESZ ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; CATALYSIS; CONTROLLED STUDY; ENZYME INACTIVATION; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; CATALYTIC DOMAIN; CLONING, MOLECULAR; DNA PRIMERS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; THERMUS;

BACILLUS SUBTILIS;

EID: 34248581677     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.04.053     Document Type: Article

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