An integrated biomarker-based strategy for ecotoxicological evaluation of risk in environmental management

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 552, Issue 1-2, 2004, Pages 247-268

  Moore, Michael N ^a   Depledge, Michael H ^b   Readman, James W ^a   Paul Leonard, D R ^c  

^a PLYMOUTH MARINE LABORATORY   (United Kingdom)

^b ENVIRONMENT AGENCY   (United Kingdom)

^c DEFRA   (United Kingdom)

Author keywords

Biomarkers; Ecosystem health; Ecotoxicology; Governance; Health status; Integrated environmental management; Lysosomal stability; Middle out approach; Modelling; Policy; RAMP; Risk; Systems biology

Indexed keywords

ALDEHYDE DEHYDROGENASE; BIOLOGICAL MARKER; CHROMIUM; COBALT; COPPER; CYTOCHROME P450; IRON; MEMBRANE PROTEIN; OIL; ORGANIC CARBON; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); TOXIN; VANADIUM; XENOBIOTIC AGENT;

ANTIOXIDANT ACTIVITY; BIOLOGICAL MONITORING; BIOTRANSFORMATION; CARCINOGENESIS; CELL; CELL DAMAGE; CHEMICAL INTERACTION; COMPUTER SIMULATION; CORRELATION ANALYSIS; COST EFFECTIVENESS ANALYSIS; DECISION MAKING; DETOXIFICATION; ECOSYSTEM; ECOTOXICOLOGY; ENVIRONMENTAL HEALTH; ENVIRONMENTAL IMPACT; ENVIRONMENTAL MANAGEMENT; ENZYME LINKED IMMUNOSORBENT ASSAY; ESTHETICS; FISH; FOREST; GENETIC SUSCEPTIBILITY; GENOTOXICITY; HEALTH STATUS; HEALTH SURVEY; HUMAN; IMMUNOASSAY; IMMUNOCHEMISTRY; INFORMATION SYSTEM; INTRACELLULAR TRANSPORT; LAW; LEARNING; LYSOSOME; MULTIDRUG RESISTANCE; MUSSEL; NONHUMAN; OIL SPILL; ORGAN; OXIDATION REDUCTION REACTION; PATHOLOGY; PHYSIOLOGY; PLANT CELL; POLICY; POLLUTANT; POLLUTION; PREDICTION; PRIORITY JOURNAL; PROGNOSIS; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; QUALITY CONTROL; QUANTITATIVE ANALYSIS; QUANTITATIVE STRUCTURE ACTIVITY RELATION; REPRODUCTION; REVIEW; RISK ASSESSMENT; RIVER; SEA POLLUTION; SOCIOECONOMICS; SPECIES COMPARISON; SURVIVAL RATE; THEORETICAL MODEL;

ANIMALS; BIOLOGICAL MARKERS; CONSERVATION OF NATURAL RESOURCES; ECOSYSTEM; ENVIRONMENTAL MONITORING; ENVIRONMENTAL POLLUTION; HUMANS; MODELS, BIOLOGICAL; RISK ASSESSMENT;

ANIMALIA;

EID: 3843148573     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2004.06.028     Document Type: Review

References (147)

1. Costanza R., d'Arge R., de Groot R., Farber S., Grasso M., Hannon B., Limburg K., Naeem S., O'Neill R.V., Paruelo J., Raskin R.G., Sutton P., van den Belt M. The value of the world's ecosystem services and natural capital. Nature. 387:1997;253-260
2. Rice J. Environmental health indicators. Ocean Coastal Man. 46:2003;235-259
3. Defra, Diffuse water pollution from agriculture, R&D Newslett. Agric. Environ. 12 (2004) 8.
4. Leonard P. The role of biological research in supporting policy needs. Mar. Environ. Res. 54:2002;209-214
5. R.T. Di Giulio, W.B. Benson (Eds.), Interconnections Between Human Health and Ecological Integrity, Society of Environmental Toxicology and Chemistry (SETAC), Pensacola, FL, 2002, 110 pp.
6. M.H. Depledge, J.J. Amaral-Mendes, B. Daniel, R.S. Halbrook, P. Kloepper-Sams, M.N. Moore, D.P. Peakall, The conceptual basis of the biomarker approach, in: D.G. Peakall, L.R. Shugart (Eds.), Biomarkers - Research and Application in the Assessment of Environmental Health, Springer, Berlin, 1993, pp. 15-29.
7. Leonard D.R.P., Mondon K.J., Segal M.G. A systematic approach to control radioactive waste discharges. J. Soc. Radiol. Prot. 13:1993;43-55
8. Leonard D.R.P., Hunt G.J. A study of fish and shellfish consumers near Sellafield: assessment of the critical groups including consideration of children. J. Soc. Radiol. Prot. 5:1985;129-138
9. B.L. Bayne, D.W. Brown, K. Burns, D.R. Dixon, A. Ivanovici, D.R. Livingstone, D.M. Lowe, M.N. Moore, A.R.D. Stebbing, J. Widdows, The Effects of Stress and Pollution on Marine Animals, Praeger, New York, 1985, 384 pp.
10. Köhler A., Wahl E., Söffker K. Functional and morphological changes of lysosomes as prognostic biomarkers of toxic liver injury in a marine flatfish (Platichthys flesus (L)). Environ. Toxicol. Chem. 21:2002;2434-2444
11. Livingstone D.R. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Pollut. Bull. 42:2001;656-666
12. Livingstone D.R., Chipman J.K., Lowe D.M., Minier C., Mitchelmore C.L., Moore M.N., Peters L.D., Pipe R.K. Development of biomarkers to detect the effects of organic pollution on aquatic invertebrates: recent molecular, genotoxic, cellular and immunological studies on the common mussel (Mytilus edulis L.) and other mytilids. Int. J. Environ. Pollut. 13:2000;56-91
13. Moore M.N. Lysosomal cytochemistry in marine environmental monitoring. Histochem. J. 22:1990;187-191
14. Moore M.N., Simpson M.G. Molecular and cellular pathology in environmental impact assessment. Aquat. Toxicol. 22:1992;313-322
15. Ringwood A.H., Hoguet J., Keppler C., Gielazyn M. Linkages between cellular biomarker responses and reproductive success in oysters, Crassostrea virginica. Mar Environ. Res. 58:2004;151-155
16. M.N. Moore, A. Kohler, D.M. Lowe, M.G. Simpson, An integrated approach to cellular biomarkers in fish, in: M.C. Fossi, C. Leonzio (Eds.), Non-Destructive Biomarkers in Vertebrates, Lewis/CRC, Boca Raton, 1994, pp. 171-197.
17. Moore M.N. Biocomplexity: the post-genome challenge in ecotoxicology. Aquat. Toxicol. 59:2002;1-15
18. D.E. Hinton, D.J. Lauren, Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure, in: J.F. McCarthy, L.K. Shugart (Eds.), Biomarkers of Environmental Contamination, Lewis Publishers, Boca Rota, 1990, pp. 17-37.
19. J.F. McCarthy, L.R. Shugart (Eds.), Biomarkers of Environmental Contamination, Lewis Publishers, Boca Raton, Ann Arbor, 1990, 457 pp.
20. Allen J.I., Moore M.N. Environmental prognostics: is the current use of biomarkers appropriate for environmental risk evaluation. Mar. Environ. Res. 58:2004;227-232
21. Kalpaxis D.L., Theos C., Xaplanteri M.A., Dinos G.P., Catsiki A.V., Leotsinidis M. Biomonitoring of Gulf of Patras, N. Peloponnesus, Greece. Application of a biomarker suite including evaluation of translation efficiency in Mytilus galloprovincialis cells. Environ. Res. 94:2004;211-220
22. Regoli F. Total; oxyradical scavenging capacity (TOSC) in polluted and translocated mussels: a predictive biomarker of oxidative stress. Aquat. Toxicol. 50:2000;351-361
23. McFadzen I., Baynes S., Hallam J., Beesley A., Lowe D. Histopathology of the skin of UV-B irradiated sole (Solea solea) and turbot (Scophthalmus maximus) larvae. Mar. Environ. Res. 50:2000;273-277
24. McFadzen I., Eufemia N., Heath C., Epel D., Moore M.N., Lowe D. Multidrug resistance in the embryos and larvae of the mussel, Mytilus edulis. Mar. Environ. Res. 50:2000;319-323
25. G.W. Winston, S.M. Adams, W.H. Benson, L.E. Gray, H.S. Matthews, M.N. Moore, S. Safe, Biological bases of similarities and differences, in: R.T. Di Giulio, W.B. Benson (Eds.), Interconnections Between Human Health and Ecological Integrity, Society of Environmental Toxicology and Chemistry (SETAC), Pensacola, FL, 2002, pp. 43-65.
26. Biganzoli E., Boracchi P., Daidone M.G., Marubini E. Flexible modelling in survival analysis. Structural biological complexity from the information provided by tumor markers. Int. J. Biol. Markers. 13:1998;107-123
27. Düchting W., Ulmer W., Ginsberg T. Cancer: a challenge for control theory and computer modelling. Eur. J. Cancer Part A. 32:1996;1283-1292
28. D.A. Lauffenburger, J.L. Linderman, Receptors: Models for Binding, Trafficking and Signaling, Oxford University Press, Oxford, 1993, 365 pp.
29. Moore M.N., Allen J.I. A computational model of the digestive gland epithelial cell of the marine mussel and its simulated responses to aromatic hydrocarbons. Mar. Environ. Res. 54:2002;579-584
30. McVeigh A., Allen J.I., Moore M.N., Dyke P., Noble D. A carbon and nitrogen flux model of mussel digestive gland epithelial cells and their simulated response to pollutants. Mar. Environ. Res. 58:2004;821-828
31. Noble D. Modelling the heart: insights. BioEssays. 24:2002;1155-1163
32. Noble D. The rise of computational biology. Nature Rev. Mol. Cell Biol. 3:2002;460-463
33. Noble D. Unraveling the genetics and mechanisms of cardiac arrhythmia. Proc. Natl. Acad. Sci. U.S.A. 99:2002;5755-5756
34. Noble D., Levin J., Scott W. Biological simulations in drug discovery. Drug Discovery Today. 4:1999;10-16
35. Chicurel M. The bigger picture. New Scientist. 164(2216):1999;38-42
36. S.A. Kauffman, The Origins of Order, Oxford University Press, Oxford, 1993, 709 pp.
37. S. Brenner, Biological computation, in: G.R. Bock, J.A. Goode (Eds.), The Limits of Reductionism in Biology, Novartis Foundation Symposium, vol. 213, 1998, pp. 106-116.
38. Schaff J., Fink C., Slepchenko B., Carson J., Loew L. A general computational framework for modeling cellular structure and function. Biophys. J. 73:1997;1135-1146
39. Howard C.V. Synergistic effects of chemical mixtures: can we rely on traditional toxicology? The Ecologist. 27:1997;192-195
40. Kanzawa F., Nishio K., Fukuoka K., Fukuda M., Kunimoto T., Saijo N. Evaluation of synergism by a novel three-dimensional model for the combined action of cisplatin and etoposide on the growth of a human small-cell lung-cancer cell line, SBC-3. Int. J. Cancer. 71:1997;311-319
41. M.H. Depledge, Rapid assessment of marine pollution (RAMP), in: Proceedings of the Vietnam-UK Joint Workshop on Marine Pollution Assessment, Hanoi, June 8, 2000, Publication for National Centre for Natural Science and Technology of Vietnam, Hanoi, Vietnam, 2000, pp. 5-16.
42. Moore M.N. A strategy for impact and risk assessment in integrated environmental management. Rev. Méd. Vét. 153:2002;507-512
43. M.H. Depledge, Interactions between heavy metals and physiological processes in estuarine invertebrates, in: P.L. Chambers, C.M., Chambers (Eds.), Estuarine Ecotoxicology, Japaga, Wicklow, Ireland, 1990, pp. 89-100.
44. Murdoch A., Kaiser K.L.E., Comba M.E., Neilson M. Particle-associated PCBs in Lake Ontario. Sci. Total. Environ. 158:1994;113-125
45. Smedes F. Sampling and partition of neutral organic contaminants in surface waters with regard to legislation. Int. J. Environ. Anal. Chem. 57:1994;215-229
46. Readman J.W., Mantoura R.F.C., Rhead M.M. The physico-chemical speciation of polycyclic aromatic hydrocarbons (PAH) in aquatic systems. Fresenius Z. Anal. Chim. 319:1984;126-131
47. M.B. Thomson, J.J. Pignatello, Mechanisms and effects of Resistant Sorption processes of organic compounds in natural particles, in: 214th ACS National Meeting of American Chemical Society, Division of Environmental Chemistry, Preprints of extended abstracts 37 (1997) 159-160.
48. Thomas D., Gustafsson B., Gustafsson Ö. Quantification of the soot-water distribution coefficient of PAHs provides mechanistic basis for enhanced sorption observations. Environ. Sci. Technol. 34:2000;5144-5151
49. Zhou J.L., Fileman T.W., Evans S.V., Donkin P., Llewellyn C.A., Readman J.W., Mantoura R.F.C., Rowland S.J. Fluoranthene and pyrene in the suspended particulate matter and surface sediments of the Humber estuary, UK. Mar. Poll. Bull. 36:1998;597-597
50. Zhou J.L., Fileman T.W., Evans S., Donkin P., Readman J.W., Mantoura R.F.C., Rowland S. The partition of flouranthene and pyrene between suspended particles and dissolved phase in the Humber Estuary: a study of the controlling factors. Sci. Total Environ. 244:1999;305-321
51. Cajaraville M.P., Abascal I., Etxeberria M., Marigómez I. Lysosomes as cellular markers of environmental pollution: time- and dose-dependant responses of the digestive lysosomal system of mussels after petroleum hydrocarbon exposure. Environ. Toxicol. Water Qual. 10:1995;1-8
52. Franke H. Substructural alterations of liver parenchymal cells induced by xenobiotics. Exp. Pathol. 39:1990;139-155
53. Halliwell W.H. Cationic amphiphilic drug-induced phospholipidosis. Toxicol. Pathol. 25:1997;53-60
54. Hawkins A.J., Day A.J. Metabolic interactions underlying the physiological and evolutionary advantages of genetic diversity. Am. Zool. 39:1999;401-411
55. Hein L., Lüllman-Rauch R., Mohr K. Human accumulation potential of xenobiotics: potential of catamphiphilic drugs to promote their accumulation via inducing lipidosis or mucopolysaccaridosis. Xenobiotica. 20:1990;1259-1267
56. Hutchins M.U., Veenhuis M., Klionsky D. Peroxisome degradation in Saccharomyces cerevisiae is dependant on machinery of macroautophagy and the Cvt pathway. J. Cell Sci. 112:1999;4079-4087
57. Luzikov V. NQuality control: from molecules to organelles. FEBS Letts. 448:1999;201-205
58. Marigómez I., Baybay-Villacorta L. Pollutant-specific and general lysosomal responses in digestive cells of mussels exposed to model organic chemicals. Aquat. Toxicol. 64:2003;235-257
59. Moore M.N., Soverchia C., Thomas M. Enhanced lysosomal autophagy of intracellular proteins by xenobiotics in living molluscan blood cells. Acta Histchem. Cytochem. 29(Suppl.):1996;947-948
60. Moore M.N., Lowe D.M., Soverchia C., Haigh S.D., Hales S.G. Uptake of a non-calorific, edible sucrose polyester oil and olive oil by marine mussels and their influence on uptake and effects of anthracene. Aquat. Toxicol. 39:1997;307-320
61. Moore M.N. The Robert Feulgen Lecture 1990. Environmental distress signals: cellular reactions to marine pollution. Prog. Histochem. Cytochem. 23:1991;1-19
62. Moore M.N., Willows R.I. A model for cellular uptake and intracellular behaviour of particulate-bound micropollutants. Mar. Environ. Res. 46:1998;509-514
63. Mortimore G.E., Poso A.R. Intracellular protein catabolism and its control during nutrient deprivation and supply. Annu. Rev. Nutr. 7:1987;539-564
64. Rashid F., Horobin R.W. Accumulation of fluorescent non-cationic probes in mitochondria of cultured-cells - observations, a proposed mechanism, and some implications. J. Microsc. 163:1991;233-241
65. Rashid F., Horobin R.W., Williams M.A. Predicting the behaviour and selectivity of fluorescent probes for lysosomes and related structures by means of structure-activity models. Histochem. J. 23:1991;450-459
66. Seglen P.O. DNA ploidy and autophagic protein degradation as determinants of hepatocellular growth and survival. Cell Biol. Toxicol. 13:1997;301-315
67. +-ATPase through proteosomal and endo-lysosomal proteolytic pathways. FASEB J. 13:1999;1751-1761
68. 2+ level in molluscan blood cells evaluated by confocal microscopy and spectrofluorimetry. Mar. Biol. 119:1994;557-564
69. Winston G.W., Moore M.N., Kirchin M.A., Soverchia C. Production of reactive oxygen species (ROS) by hemocytes from the marine mussel, Mytilus edulis. Comp. Biochem. Physiol. 113C:1996;221-229
70. Leonard D.R.P., Pentreath R.J. Further 237Pu experiments with the plaice Pleuronectes platessa: sub-cellular distribution of plutonium in the liver. Mar. Biol. 63:1981;67-71
71. Bjerregaard P., Depledge M.H. Cadmium accumulation in Littorina littorea, Mytilus edulis and Carcinus maenas: the influence of salinity and calcium ion concentrations. Mar. Biol. 119:1994;385-395
72. Kortenkamp A., Altenburger R. Synergisms with mixtures of xenoestrogens: a reevaluation using the method of isoboles. Sci. Total Environ. 221:1998;59-73
73. Lyons B.P., Pascoe C.K., McFadzen I.R.B. Phototoxicity of pyrene and benzo[a]pyrene to embryo-larval stages of the pacific oyster Crassostrea gigas. Mar. Environ. Res. 54:2002;627-631
74. Aga D.S., Thurman E.M. Environmental immunoassays: alternative techniques for soil and water analysis. ACS Symp. Ser. 657:1997;1-20
75. Sherry J. Environmental immunoassays and other bioanalytical methods: overview and update. Chemosphere. 34:1997;1011-1025
76. Wells P.G., Depledge M.H., Butler J.N., Manock J.J., Knap A.H. Rapid toxicity assessment and biomonitoring of marine contaminants - exploiting the potential of rapid biomarker assays and microscale toxicity tests. Mar. Pollut. Bull. 42:2001;799-804
77. Cheung V.V., Wedderburn R.J., Depledge M.H. Molluscan lysosomal responses as a diagnostic tool for detection of a pollution gradient in Tolo Harbour, Hong Kong. Mar. Environ. Res. 46:1998;237-241
78. Hauton C., Hawkins L.E., Hutchinson S. The use of neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L.). Comp. Biochem. Physiol. 199:1998;619-623
79. Lowe D.M., Fossato V.U., Depledge M.H. Contaminant induced lysosomal membrane damage in blood cells of mussels M. galloprovincialis from the Venice Lagoon: an in vitro study. Mar. Ecol. Prog. Ser. 129:1995;189-196
80. Svendseb C., Weeks J.M. The use of a lysosome assay for the rapid assessment of cellular stress from copper to the freshwater snail Viviparus contectus (Millet). Mar. Poll. Bull. 31:1995;139-142
81. Wedderburn J., Cheung V., Bamber S., Bloxham M., Depledge M.H. Biomarkers of histochemical and cellular stress in Carcinus maenas: an in situ field study. Mar. Environ. Res. 46:1998;321-324
82. Kurelec B., Pivcevic B. Evidence for a multixenobiotic resistance mechanism in the mussel Mytilus galloprovincialis. Aquat. Toxicol. 19:1991;291-302
83. Minier C., Moore M.N. Multixenobiotic resistance in mussel blood cells. Mar. Environ. Res. 42:1996;389-392
84. Stegeman J.J., Lech J.J. Cytochrome P-450 mono-oxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure. Environ. Health Perspect. 90:1991;93-100
85. Depledge M.H. The rational basis for detection of the early effects of marine pollution using physiological indicators. Ambio (Royal Swedish Academy of Sciences). 18:1989;301-302
86. Livingstone D.R. Organic xenobiotic metabolism in marine invertebrates. Adv. Comp. Environ. Physiol. 7:1991;45-185
87. Livingstone D.R. Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. J. Chem. Tech. Biotechnol. 57:1993;195-211
88. Livingstone D.R., Garcia Martinez P., Michel X., Narbonne J.F., O'Hara S.C.M., Ribera D., Winston G.W. Oxyradical production as a pollution-mediated mechanism of toxicity in the common mussel, Mytilus edulis L., and other molluscs. Funct. Ecol. 4:1990;413-424
89. Mason R. Free radical metabolites of foreign compounds and their toxicological significance. Rev. Biochem. Toxicol. 87:1990;237-243
90. Dailianis S., Domouhtsidou G.P., Raftopoulou E., Kaloyianni M., Dimitriadis V.K. Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity and a signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring. Mar. Environ. Res. 56:2003;443-470
91. Nebert D.W., Petersen D.D., Fornace A.J. Jr. Cellular responses to oxidative stress: the [Ah] gene battery as a paradigm. Environ. Health Perspect. 88:1990;13-25
92. Dixon D.R., Pruski A.M., Dixon L.R.J., Jha A.N. Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis. 17:2002;495-507
93. Jha A.N., Cheung V.V., Foulkes M.E., Hill H.J., Depledge M.H. Detection of genotoxins in the marine environment: adoption and evaluation of an integrated approach using the larval stages of the marine mussel, Mytilus edulis. Mutat. Res. 464:2000;213-228
94. Albertini R.J., Nicklas J.A., O'Neill J.P. Future research directions for evaluating human genetic and cancer risk from environmental exposures. Environ. Health Perspect. 104(Suppl. 3):1996;503-510
95. 32P -postlabelling analysis of DNA adducts and EROD induction as biomarkers of genotoxin exposure in dab (Limanda limanda) from British coastal waters Mar. Environ. Res. 50:2000;575-579
96. Kurelec B. The genotoxic disease syndrome. Mar. Environ. Res. 35:1993;341-348
97. D.R.P. Leonard, R.J. Law, C.A. Kelly, Responding to the Sea Empress oil spill, in: Proceedings of the International Symposium on Marine Pollution, IAEA SM-354/86 IAEA-TECDOC-1094, 1998, pp. 177-182.
98. Nuwaysir E.F., Bittner M., Trent J., Barrett J.C., Afshari C.A. Microarrays and toxicology: the advent of toxicogenomics. Mol. Carcinogenesis. 24:1999;153-159
99. Pennie W.D., Tugwood J.D., Oliver G.J., Kimber I. The principles and practice of toxicogenomics: applications and opportunities. Toxicol. Sci. 54:2000;277-283
100. ECETOC, 2001, White Paper on Genomics, Transcript Profiling, Proteomics and Metabonomics (GTPM) - An Introduction, ECETOC Document No. 42, European Centre for the Ecotoxicology and Toxicology of Chemicals, Brussels, Belgium, 2001, 27 pp.
101. Snape J.R., Maund S.J., Pickford D.B., Hutchinson T.H. Ecotoxicogenomics: the challenge of integrating genomics into aquatic and terrestrial ecotoxicology. Aquat. Toxicol. 67:2004;143-154
102. Bannasch P., Enzmann H., Klimek F., Weber E., Zerban H. Significance of sequential cellular changes inside and outside foci of altered hepatocytes during hepatocarcinogenesis. Toxicol. Pathol. 4:1989;617-628
103. Cajaraville M.P., Cancio I., Ibabe A., Orbea A. Peroxisome proliferation as a biomarker in environmental pollution assessment. Microsc. Res. Tech. 61:2003;117-120
104. Galloway T.S., Sanger R.C., Smith K.L., Fillmann G., Readman J.W., Ford T.E., Depledge M.H. Rapid assessment of marine pollution using multiple biomarkers and chemical immunoassays. Environ. Sci. Technol. 36:2002;2219-2226
105. Köhler A., Deisemann H., Lauritzen B. Ultrastructural and cytochemical indices of toxic injury in dab liver. Mar. Ecol. Prog. Ser. 91:1992;141-153
106. M.N. Moore, G. Topping, P.-E. Minhea, S. Kiryanov, V. Mikhailov, Design of a monitoring programme for the Black Sea: contaminant levels and biological effects, in: L. Mee, G. Topping (Eds.), Black Sea Pollution Assessment, UN Publications, New York, 1998, pp. 293-299.
107. Lowe D.M., Moore M.N., Evans B. Contaminant impact on interactions of molecular probes with lysosomes in living hepatocytes from Dab (Limanda limanda). Mar. Ecol. Prog. Ser. 91:1992;135-140
108. Moore M.N. Molecular cell pathology of pollutant induced liver injury in flatfish: use of fluorescent probes. Mar. Ecol. Prog. Ser. 91:1992;127-133
109. A.J. Lawrence, A. Arukwe, M.N. Moore, M. Sayer, J. Thain, Molecular/cellular processes and the physiological response to stress, in: A. Lawrence, K. Hemingway (Eds.), Effects of Pollution on Fish - Molecular Effects and Population Responses, Blackwell Science Ltd., Oxford, 2003, pp. 83-133.
110. Lowe D.M., Soverchia C., Moore M.N. Lysosomal membrane responses in the blood and digestive cells of mussels experimentally exposed to flouranthene. Aquat. Toxicol. 33:1995;105-112
111. Hunter P. Putting Humpty Dumpty back together again. The Scientist. 17:2003;20-21
112. Smaill B.H., McGiffin D.C., LeGrice I.J., Young A.A., Hunter P.J., Galbraith A.J. The effect of synthetic patch repair of coarctation on regional deformation of the aortic wall. J. Thorac. Cardiov. Sur. 120:2000;1053-1063
113. Rice J.J., Jafri M.S. Modelling calcium handling in cardiac cells. Philos. Trans. Roy. Soc. 359A:2001;1143-1157
114. P.J. Hunter, P.M.F. Nielsen, D. Bullivant, The IUPS physiome project, in: "In Silico" Simulation of Biological Processes, Novartis Foundation Symposium 247 (2002) 207-221.
115. D.d'A. Laffoley, J. Burt, P. Gilland, J. Baxter, D.W. Connor, M. Hill, J. Breen, M. Vincent, E. Maltby, Adopting an ecosystem approach for the improved stewardship of the maritime environment, English Nat. Res. Rep. 538 (2003) 20.
116. M.N. Moore, D.M. Lowe, R.J. Wedderburn, T.L. Wade, G. Balashov, H. Buyukgungor, Y. Daurova, Y. Denga, E. Kostylev, P. Mihnea, S. Moncheva, S. Tabagari, C. Ciocan, H. Ozkoc, M.H. Depledge, International Mussel Watch (UNESCO/IOC) in the Black Sea: a pilot study for biological effects and contaminant residues, in: S. Besiktepe, I. Unluata (Eds.), Environmental Degradation of the Black Sea: Challenges and Remedies, NATO Advanced Research Workshop, Kluwer Academic, New York, Dordrecht, 1999, pp. 273-289.
117. M.N. Moore, T.L. Wade, D.M. Lowe, R.J. Wedderburn, G. Balashov, H. Büyükgüngör, Y. Daurova, Y. Denga, E. Kostylev, P. Mihnea, S. Moncheva, S. Tabagari, C. Ciocan, H. Özkoc, M.H. Depledge, The UNESCO/IOC "Black Sea Mussel Watch Pilot Study": biological effects and contaminant residues, in: L. Mee, G. Topping (Eds.), Black Sea Pollution Assessment, UN Publications, New York, 1998, pp. 279-292.
118. K.E. Drexler, Nanosystems: Molecular Machinery, Manufacturing and Computation, Wiley-Interscience, New York, 1992, 556 pp.
119. Howard V. Small particles - big problems. Int. Lab. News. 34(2):2004;28-29
120. M. Gross, Travels to the Nanoworld: Miniature Machinery in Nature and Technology, Plenum Trade, New York, 1999, 254 pp.
121. Perrin R.M. Crop protection: taking stock for the new millennium. Crop Protect. 16:1997;449-456
122. Darmency H. The impact of hybrids between genetically modified plant crop plants and their related species: introgression and weediness. Mol. Ecol. 3:1994;37-40
123. Dunwell J.M., Paul E.M. Impact of genetically modified crops in agriculture. Outlook Agric. 19:1990;103-110
124. Drexler K.E. Molecular nanomachines: physical principles and implementation strategies. Ann. Rev. Biophys. Biomol. Struct. 23:1994;377-405
125. Kitov P., Sadowska J.M., Mulvey G., Armstrong G.D., Ling H., Pannu N.S., Read R.J., Bundle D.R. Shiga-like toxins are neutralized by tailored multivalent carbohydrate ligands. Nature. 403:2000;669-672
126. Brigger I., Dubernet C., Couvreur P. Nanoparticles in cancer therapy and diagnosis. Adv. Drug Delivery Rev. 54:2002;631-651
127. Gatti A.M., Rivasi F. Biocompatibility of micro- and nanoparticles. Part I. In liver and kidney. Biomaterials. 23:2002;2381-2387
128. Gould P. Nanoparticles probe biosystems. Mater. Today. 7(2):2004;36-43
129. Panyam J., Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissues. Adv. Drug Delivery Rev. 55:2003;329-347
130. Cajaraville M.P., Bebianno M.J., Blasco J., Porte C., Sarasquete C., Viarengo A. The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian peninsula: a practical aaproach. Sci. Tot. Environ. 247:2000;295-311
131. M.N. Moore, Z. Csizer, Integrated coastal zone management as a toolbox for environmentally sustainable industrial development in coastal areas, in: L.F. Cassar (Ed.), Integrated Coastal Zone Management, UNIDO, Vienna, 2001, pp. 161-166.
132. ICES International Council for the Exploration of the Seas (ICES), Report of the Working Group on the Biological Effects of Contaminants, ICES, Copenhagen, 1997.
133. C.J. Bayne, M.N. Moore, Non-lymphoid immunologic defenses in aquatic invertebrates and their value as indicators of aquatic pollution, in: J.T. Zelikoff (Ed.), EcoToxicology: Responses, Biomarkers and Risk Assessment, Published for OECD by SOS Publications, Fair Haven, New Jersey, 1998, pp. 243-261.
134. Grundy M.M., Moore M.N., Howell S.M., Ratcliffe N.A. Phagocytic reduction and effects on lysosomal membranes of polycyclic aromatic hydrocarbons, in haemocytes of Mytilus edulis. Aquat. Toxicol. 34:1996;273-290
135. Grundy M.M., Ratcliffe N.A., Moore M.N. Immune inhibition in marine mussels by polycyclic aromatic hydrocarbons. Mar. Environ. Res. 42:1996;187-190
136. S. Ferson, T.F. Long, Conservative uncertainty propagation in environmental risk assessments, in: J.S. Hughes et al. (Eds.), Environmental Toxicology & Risk Assessment, ASTM, Philadelphia, 1995, pp. 97-110.
137. Warne M.St.J., Hawker D.W. The number of components in a mixture determines whether synergistic and antagonistic or additive toxicity predominate: the funnel hypothesis. Ecotox. Environ. Safety. 31:1995;23-28
138. J.L. Casti, Complexification: Explaining a Paradoxical World Through the Science of Surprise, Abacus, London, 1994, 320 pp.
139. J. Maddox, What Remains to be Discovered, The Free Press, New York, 1998, 434 pp.
140. Depledge M.H. Genotype toxicity: implications for individuals and populations. Environ. Health Perspect. 102:1994;101-104
141. Depledge M.H. Recovery of ecosystems and their components following exposure to pollution. J. Aquat. Ecosyst. Health. 6:1999;199-206
142. Browne R.A., Moller V., Forbes V.E., Depledge M.H. Estimating genetic and environmental components of variance using sexual and clonal Artemia. J. Exp. Mar. Biol. Ecol. 267:2002;107-119
143. Brown R.J., Galloway T.S., Lowe D., Browne M.A., Dissanayake A., Jones M.B., Depledge M.H. Differential sensitivity of three marine invertebrates to copper assessed using multiple biomarkers. Aquat. Toxicol. 66:2004;267-278
144. Galloway T.S., Brown R.J., Browne M.A., Dissanayake A., Lowe D., Jones M.B., Depledge M.H. A multibiomarker approach to environmental assessment. Environ. Sci. Technol. 38:2004;1723-1731
145. Watson G., Andersen O.K., Galloway T.S., Depledge M.H. Rapid assessment of polycyclic aromatic hydrocarbon (PAH) exposure in decapod crustaceans by fluorimetric analysis of urine and haemolymph. Aquat. Toxicol. 67:2004;127-142
146. Fillmann G., Sanger R.C., Depledge M.H., Readman J.W. Relative performance of immunochemical (enzyme-linked immunosorbent assay) and gas chromatography-electron-capture detection techniques to quantify polychlorinated biphenyls in mussel tissues. Anal. Chim. Acta. 461:2002;75-84
147. Fillmann G., Watson G.M., Howsam M., Francioni E., Depledge M.H., Readman J.W. Urinary PAH metabolites as biomarkers of exposure in aquatic environments. Environ. Sci. Technol. 38:2004;2649-2656