Rapid assessment of polycyclic aromatic hydrocarbon (PAH) exposure in decapod crustaceans by fluorimetric analysis of urine and haemolymph

Aquatic Toxicology

Volumn 67, Issue 2, 2004, Pages 127-142

  Watson, Giles M ^a   Andersen, Odd Ketil ^b   Galloway, Tamara S ^a   Depledge, Michael H ^a,c  

^a UNIVERSITY OF PLYMOUTH   (United Kingdom)

^b RF Rogaland Research   (Norway)

^c ENVIRONMENT AGENCY   (United Kingdom)

Author keywords

Biotransformation; ELISA; Haemolymph; HPLC F; Metabolites; Non destructive biomarker; PAH; Shore crab; Urine

Indexed keywords

POLYCYCLIC AROMATIC HYDROCARBON; PYRENE;

BIOTRANSFORMATION; CRUSTACEAN; METABOLISM; PAH; POLLUTION EXPOSURE;

ARTICLE; BIOAVAILABILITY; BIOLOGICAL MONITORING; BIOTRANSFORMATION; COASTAL WATERS; CONTROLLED STUDY; CRAB; CRUSTACEA; DECAPODA (CRUSTACEA); ELECTRIC POTENTIAL; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPOSURE; FLUOROMETRY; HEMOLYMPH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MARINE ENVIRONMENT; METABOLISM; NONHUMAN; PRIORITY JOURNAL; SAMPLING; SYNCHRONOUS SCANNING FLUORESCENCE; TECHNIQUE; URINALYSIS;

ANALYSIS OF VARIANCE; ANIMALS; BRACHYURA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENVIRONMENTAL EXPOSURE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUOROMETRY; GREAT BRITAIN; POLYCYCLIC HYDROCARBONS, AROMATIC; PYRENES; TIME FACTORS;

CARCINUS MAENAS; CRUSTACEA; DECAPODA (CRUSTACEA); GRAPSIDAE;

EID: 1542345505     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aquatox.2003.11.006     Document Type: Article

References (45)

1. Aas E., Klungsoyr J. PAH metabolites in bile and EROD activity in North Sea fish. Mar. Environ. Res. 46(1-5):1998;229-232.
2. Aas E., Beyer J., Goksoyr A. PAH in fish bile detected by fixed wavelength fluorescence. Mar. Environ. Res. 46(1-5):1998;225-228.
3. Aas E., Beyer J., Goksoyr A. Fixed wavelength fluorescence (FF) of bile as a monitoring tool for polyaromatic hydrocarbon exposure in fish: an evaluation of compound specificity, inner filter effect and signal interpretation. Biomarkers. 5(1):2000a;9-23.
4. Aas E., Baussant T., Balk L., Liewenborg B., Andersen O.K. PAH metabolites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic cod. Aquat. Toxicol. 51(2):2000b;241-258.
5. Ariese F., Kok S.J., Verkakik M., Goojier C., Velthorst N.H., Hofstraat J.W. Synchronous fluourescence spectrometry of fish bile: a rapid screening method for the biomonitoring of PAH exposure. Aquat. Toxicol. 26:1993;273-286.
6. Bamber S.D., Naylor E. Sites of release of putative sex pheremone and sexual behaviour in female Carcinus maenas (Crustacea Decapoda). East Coast Shelf Sci. 44:1997;195-202.
7. Beyer J., Aas E., Borgenvik H.K., Ravn P. Bioavailability of PAH in Effluent water from an aluminium works evaluated by transplant caging and biliary fluorescence measurements of Atlantic cod (Gadus morhua L.). Mar. Environ. Res. 46(1-5):1998;233-236.
8. Beyer J., Sandvik M., Hylland K., Fjeld E., Egaas E., Aas E., Skare J.U., Goksoyr A. Contaminant accumulation and biomarker responses in flounder (Platichthys flesus L.) and Atlantic cod (Gadus morhua L.) exposed by caging to polluted sediments in Sorfjorden, Norway. Aquat. Toxicol. 36:1996;75-98.
9. Burns K.A., Ehrhardt M.G., MacPherson J., Tierney J.A., Kananen G., Connelly D. Organic and trace metal contaminants in sediments, seawater and organisms from two Bermudan harbours. J. Exp. Mar. Biol. Ecol. 138:1990;9-34.
10. Camus L., Aas E., Borseth J.F. Ethoxyresorufin-O-deethylase activity and fixed wavelength fluorescence detection of PAHs metabolites in bile in turbot (Scophthalamus maximus L.) exposed to a dispersed topped crude oil in a continuous flow system. Mar. Environ. Res. 46:1998;29-32.
11. Depledge, M.H., 2000. Rapid assessment of marine pollution (RAMP). In: Que, B.B., Depledge, M.H., Hoang, T.V., Phuong, N.H. (Eds.), Proceedings of the Vietnam-U.K Joint Workshop on Marine Pollution Assessment, Hanoi, 8 June 2000.
12. Escartin E., Porte C. Biomonitoring of PAH pollution in high altitude mountain lakes through the analysis of fish bile. Environ. Sci. Technol. 33:1999;406-409.
13. Fillmann G., Watson G.M., Francioni E., Readman J.W., Depledge M.H. A non-destructive assessment of the exposure of crabs to PAH using ELISA analyses of their urine and haemolymph. Mar. Environ. Res. 54:2002;823-828.
14. Fillmann, G., Watson, G.M., Howsam, M., Francioni, E., Depledge, M.H., Readman, J.W., 2003. Urinary PAH metabolites as biomarkers of exposure in aquatic environments - applicability of immunochemical techniques. Environ. Sci. Technol., submitted for publication.
15. Galloway T.S., Sanger R.C., Smith K.L., Fillman G., Readman J.W., Ford T.E., Depledge M.H. Rapid assessment of marine pollution using multiple biomarkers and chemical immunoassays. Environ. Sci. Technol. 36(10):2002;2219-2226.
16. Hellou J., Upshall C. Monocyclic aromatic hydrocarbons in bile of flounder exposed to petroleum oil. Int. J. Environ. Anal. Chem. 60:1995;101-111.
17. Hufnagle L.C. Jr., Camara ta S.E., Ernest D., Krone C.A., Chan S.-L., Krahn M.M. Development and application of a high-performance liquid chromatography screening method for aromatic compounds in invertebrate tissues. Arch. Environ. Contam. Toxicol. 37:1999;220-226.
18. James, M.O., 1989. Biotransformation and disposition of PAH in aquatic invertebrates. In: U. Varanasi (Ed.), Metabolism of PAH in the Aquatic Environment. CRC Press, Boca Raton, FL, pp. 69-91.
19. James M.O. Conjugation of organic pollutants in aquatic species. Environ. Health Perspect. 71:1987;97-103.
20. James M.O., Altman A.H., Li C-L.J., Schell J.D. Jr. Biotransformation, hepatopancreas DNA binding and pharmacokinetics of benzo(a)pyrene after oral and parenteral administration to the American lobster, Homarus americanus. Chem.-Biol. Interact. 95:1995;141-160.
21. Johnston E.P., Baumann P.C. Analysis of fish bile with HPLC-flourescence to determine environmental exposure to benzo(a)pyrene. Hydrobiologia. 188-189:1989;561-566.
22. Krahn M.M., Rhodes L.D., Myers M.S., Moore L.K., MaCleod W.D., Malins D.C. Associations between metabolites of aromatic compounds in bile and the occurrence of hepatic lesions in English sole (Parophyrus vetulus) from Puget Sound, Washington. Arch. Environ. Contam. Toxicol. 15:1986;61-67.
23. Krahn M.M., Myers M.S., Burrows D.G., Malins D.C. Determination of metabolites of xenobiotics in the bile of fish from polluted waterways. Xenobiotica. 14(8):1984;633-646.
24. Law F.C.P., Meng J.X., He Y.T., Chui Y.C. Urinary and biliary metabolites of pyrene in rainbow trout (Oncorhynchus mykiss). Xenobiotica. 24(3):1994;221-229.
25. Lee R.F., Ryan C., Neuhauser M.L. Fate of petroleum hydrocarbons taken up from food and water by the blue crab Callinectus sapidus. Mar. Biol. 37:1976;363-370.
26. Li C.L.J., James M.O. Glucose and sulfate conjugations of phenol, beta-napthol and 3-hydroxybenzo(a)pyrene by the American lobster, Homarus americanus. Aquat. Toxicol. 26(1-2):1993;57-72.
27. Lin E.L.C., Cormier S.C., Torsella J.A. Fish biliary polycyclic aromatic hydrocarbon metabolites estimated by fixed-wavelength fluorescence: comparison with HPLC-fluorescent detection. Ecotox. Environ. Saf. 35:1996;16-23.
28. Lin E.L.C., Cormier S.C., Racine R.N. Synchronous flourometric measurement of metabolites of polycyclic aromatic hydrocarbons in the bile of brown bullhead. Environ. Toxicol. Chem. 13(5):1994;707-715.
29. McElroy A.E., Colarusso P.D. Disposition of dietary benzo(a)pyrene in aquatic species: winter flounder (Pseudopleuronectes americanus) and Green crab (Carcinus maenas). Bull. Mt. Desert Island Biol. Lab. 27:1987;45-47.
30. McElroy, A.E., Farrington, J.W., Teal, J.M., 1989. Bioavailability of polycyclic aromatic hydrocarbons in the aquatic environment. In: U. Varanasi (Ed.), Metabolism of PAH in the Aquatic Environment. CRC Press, Boca Raton, FL, pp. 1-39.
31. Miller H.C., Mills G.N., Bembo D.G., MacDonald J.A., Evans C.W. Induction of cytochrome P4501A (CYP1A) in Trematomus bernacchii as an indicator of environmental pollution in Antarctica: assessment by quantitative RT-PCR. Aquat. Toxicol. 44:1999;183-193.
32. Naes K., Knutzen J., Berglind L. Occurrence of PAH in marine organisms and sediments from smelter discharge in Norway. Sci. Total Environ. 163:1995;93-106.
33. Onuska, F.I., 1989. Analysis of polycyclic aromatic hydrocarbons in environmental samples. In: Afghan, B.K., Alfred, S.Y.C. (Eds.), Analysis of Trace Organics in the Aquatic Environment. CRC Press, Boca Raton, FL, pp. 206-241.
34. Pritchard J.B., Bend J.R. Relative roles of metabolism and renal excretory mechanisms in xenobiotic elimination by fish. Environ. Health Perspect. 90:1991;85-92.
35. Solbakken J.E., Palmork K.H., Neppelberg T., Scheline R.R. Urinary and biliary metabolites of phenanthrene in the coalfish (Pollachius virens). Acta Pharmacol. Toxicol. 46:1980;127-132.
36. Stegeman J.J., Lech J.J. Cytochrome P-450 monooxygenase systems in aquatic species: carcinogen metabolism and biomarkers for carcinogen and pollutant exposure. Environ. Health Perspect. 90:1991;101-109.
37. Stroomberg G.J., Ariese F., van Gestel C.A.M., van Hattum B., Velthorst N.H., van Straalen N.M. Pyrene biotransformation products as biomarkers of polycyclic aromatic hydrocarbon exposure in terrestrial isopoda: concentration-response relationship, and field study in a contaminated forest. Environ. Toxicol. Chem. 22(1):2003;224-231.
38. Stroomberg G.J., de Knecht J.A., Ariese F., van Gestel C.A.M., Velthorst N.H. Pyrene metabolites in the hepatopancreas and gut of the isopod Porcellio scaber, a new biomarker for polycyclic aromatic hydrocarbon exposure in terrestrial ecosystems. Environ. Toxicol. Chem. 18:1999;2217-2224.
39. Stroomberg G.J., Reuther C., Kozin I., van Brummelen T.C., van Gestel C.A.M., Gooijer C., Cofino W.P. Formation of pyrene metabolites by the terrestrial isopod Porcellio scaber. Chemosphere. 33(10):1996;1905-1914.
40. Sundt H., Goksoyr A. In vivo and in vitro biotransformation of polycyclic aromatic hydrocarbons in the edible crab, Cancer pagurus. Mar. Environ. Res. 46(1-5):1998;515-519.
41. UNEP/IOC/IAEA, 1992. Determination of petroleum hydrocarbons in sediments. Reference Methods for Marine Pollution Studies 20, UNEP, 75 pp.
42. Uthe J.F., Musial C.J. Polycyclic aromatic hydrocarbon contamination of American lobster (Homarus americanus), in the proximity of a coal coking plant. Bull. Environ. Contam. Toxicol. 37(5):1986;730-738.
43. Vahakangas K., Haugen A., Harris C.C. An applied synchronous fluorescence spectrophotometric assay to study benzo[a]pyrene-diolepoxide-DNA adducts. Carcinogenesis. 6:1985;1109-1116.
44. Wells P.G., Depledge M.H., Butler J.N., Manock J.J., Knap A.H. Rapid toxicity assessment and biomonitoring of marine contaminants - exploiting the potential of rapid biomarker assays and microscale toxicity tests. Mar. Pollut. Bull. 42(10):2001;799-804.
45. Woodhead R.J., Law R.J., Matthiessen P. Polycyclic aromatic hydrocarbons in surface sediments around England and Wales, and their possible biological significance. Mar. Pollut. Bull. 38(9):1999;773-790.