Distribution of eukaryotic picoplankton assemblages across hydrographic fronts in the Southern Ocean, studied by denaturing gradient gel electrophoresis

Limnology and Oceanography

Volumn 49, Issue 4 I, 2004, Pages 1022-1034

  Díez, Beatriz ^a,b   Massana, Ramon ^a   Estrada, Marta ^a   Pedrós Alió, Carlos ^a  

^a INSTITUT DE CIÈNCIES DEL MAR   (Spain)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

COMMUNITY COMPOSITION; DNA FINGERPRINTING; PHYTOPLANKTON; PICOPLANKTON; SPATIAL DISTRIBUTION; TRANSECT;

SOUTHERN OCEAN;

ALVEOLATA; BACILLARIOPHYTA; CERCOMONADIDA; DINOPHYCEAE; EUKARYOTA; HAPTOPHYCEAE; PRASINOPHYCEAE; STRAMENOPILES;

EID: 3843126289     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.2004.49.4.1022     Document Type: Article

References (29)

1. ALTSCHUL, S. F., T. L. MADDEN, A. A. SCHÄFFER, J. ZHANG, Z. ZHANG, W. MILLER, AND D. J. LIPMAN. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
2. CARON, D. A., E. R. PEELE, E. L. LIM, AND M. R. DENNETT. 1999. Picoplankton and nanoplankton and their trophic coupling in the surface waters of the Sargasso Sea south of Bermuda. Limnol. Oceanogr. 44: 259-272.
3. CASAMAYOR, E. O., C. PEDRÓS-ALIÓ, G. MUYZER, AND R. AMANN. 2002. Microheterogeneity in 16S ribosomal DNA-defined bacterial populations from a stratified planktonic environment is related to temporal changes and to ecological adaptations. Appl. Environ. Microbiol. 68: 1706-1714.
4. _, H. SCHÄFER, L. BAN̄ERAS, C. PEDRÓS-ALIÓ, AND G. MUYZER. 2000. Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes: A comparison of microscopy and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 66: 499-508.
5. DÍEZ, B., C. PEDRÓS-ALIÓ, T. L. MARSH, AND R. MASSANA. 2001a. Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques. Appl. Environ. Microbiol. 67: 2942-2951.
6. _, AND R. MASSANA. 2001b. Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by Small-Subunit rRNA gene cloning and sequencing. Appl. Environ. Microbiol. 67: 2932-2941.
7. DOVAL, M. D., X. A. ÁLVAREZ-SALGADO, J. M. GASOL, L. M. LORENZO, I. MIRÓN, F. G. FIGUEIRAS, AND C. PEDRÓS-ALIÓ. 2001. Dissolved and suspended organic carbon in the Atlantic sector of the Southern Ocean. Stock dynamics in upper ocean waters. Mar. Ecol. Prog. Ser. 223: 27-38.
8. ESTRADA, M., AND M. DELGADO. 1990. Summer phytoplankton distribution in the Weddell sea. Polar Biol. 10: 441-449.
9. GIOVANNONI, S. J., AND M. S. RAPPÉ. 2000. Evolution, diversity, and molecular ecology of marine prokaryotes, pp. 47-84. In D. L. Kirchman [ed.], Microbial ecology of the oceans. Wiley-Liss.
10. GUILLOU, L., W. EIKREM, M.-J. CHRÉTIENNOT-DINET, F. LE GALL, R. MASSANA, K. ROMARI, C. PEDRÓS-ALIÓ, AND D. VAULOT. In press. Diversity of picoplanktonic prasinophytes assessed by direct nuclear SSU rDNA sequencing of environmental samples and novel isolates retrieved from oceanic and coastal marine environments. Protist.
11. LI, W. K. W. 1994. Primary production of prochlorophytes, cyanobacteria, and eukaryotic ultraphytoplankton: Measurements from flow cytometric sorting. Limnol. Oceanogr. 39: 169-175.
12. LÓPEZ-GARCÍA, P., F. RODRÍGUEZ-VALERA, C. PEDRÓS-ALIÓ, AND D. MOREIRA. 2001. Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton. Nature 409: 603-607.
13. MASSANA, R., E. F. DELONG, AND C. PEDRÓS-ALIÓ. 2000. A few cosmopolitan phylotypes dominate planktonic archaeal assemblages in widely different oceanic provinces. Appl. Environ. Microbiol. 66: 1777-1787.
14. _, L. GUILLOU, B. DÍEZ, AND C. PEDRÓS-ALIÓ. 2002. Unveiling the organisms behind novel eukaryotic ribosomal DNA sequences from the Ocean. Appl. Environ. Microbiol. 68: 4554-4558.
15. MOON-VAN DER STAAY, S. Y., R. DE WACHTER, AND D. VAULOT. 2001. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409: 607-610.
16. MOORE, L. R., G. ROCAP, AND S. W. CHISHOLM. 1998. Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes. Nature 393: 464-467.
17. MURRAY, A. E., J. HOLLIBAUGH, AND C. ORREGO. 1996. Phylogenetic composition of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rRNA fragments. Appl. Environ. Microbiol. 62: 2676-2680.
18. MUYZER, G., E. C. DEWAAL, AND A. G. UITTERLINDEN. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rRNA. Appl. Environ. Microbiol. 59: 695-700.
19. ORSI, A. H., T. WHITWORTH, III, AND W. D. NOWLIN, JR. 1995. On the meridional extent and fronts of the Antarctic Circumpolar Current. Deep-Sea Res. I 42: 641-673.
20. PARTENSKY, F., L. GUILLOU, N. SIMON, AND D. VAULOT. 1997. Recent advances in the use of molecular techniques to assess the genetic diversity of marine photosynthetic microorganisms. Vie Milieu 47: 367-374.
21. RAPPÉ, M. S., M. T. SUZUKI, K. L. VERGIN, AND S. J. GIOVANNONI. 1998. Phylogenetic diversity of ultraplankton plastid small-subunit rRNA genes recovered in environmental nucleic acid samples from the Pacific and Atlantic coasts of the United States. Appl. Environ. Microbiol. 64: 294-303.
22. SCHAUER, M., V. BALAGUÉ, C. PEDRÓS-ALIÓ, AND R. MASSANA. 2003. Temporal changes in bacterioplankton diversity in the oligotrophic Mediterranean Coast. Aquat. Microb. Ecol. 31: 163-174.
23. _, R. MASSANA, AND C. PEDRÓS-ALIÓ. 2000. Spatial differences in bacterioplankton composition along the Catalan coast (NW Mediterranean) assessed by molecular fingerprinting. FEMS Microbiol. Ecol. 33: 51-59.
24. SIMON, N., R. G. BARLOW, D. MARIE, F. PARTENSKY, AND D. VAULOT. 1994. Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry. J. Phycol. 30: 922-935.
25. THOMSEN, H. A. 1986. A survey of the smallest eukaryotic organisms of the marine phytoplankton. Can. Bull. Fish. Aquat. Sci. 214: 121-158.
26. THRONDSEN, J., AND S. KRISTIANSEN. 1991. Micromonas pusilla (Prasinophceae) as part of pico- and nanoplankton communities of the Barents Sea, pp. 201-207. In E. Sakshomy, C. C. E. Hopkins, and N. A. Oeritsland [eds.], Proceedings of the Polar Marine Symposium on Polar Marine Ecology, vol. 10. Trondheim, Norway.
27. UTERMÖHL, H. 1958. Zur Vervollkommung der quantitativen Phytoplankton-Methodik. Mitt. Int. Ver. Theor. Angew. Limnol. 9: 1-38.
28. VAN HANNEN, E. J., M. P. VAN AGTERVELD, H. J. GONS, AND H. J. LAANBROEK. 1998. Revealing genetic diversity of eukaryotic microorganisms in aquatic environments by denaturing gradient gel electrophoresis. J. Phycol. 34: 206-213.
29. YENTSCH, C. S., AND D. W. MENZEL. 1963. A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence. Deep-Sea Res. 10: 221-231.