Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism

Journal of Biological Chemistry

Volumn 279, Issue 30, 2004, Pages 31033-31040

  Ohtaki, Akashi ^a   Mizuno, Masahiro ^a   Tonozuka, Takashi ^a   Sakano, Yoshiyuki ^a   Kamitori, Shigehiro ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSTS; ENZYMES; HYDROLYSIS; STRUCTURAL ANALYSIS; SUBSTRATES;

CAVITIES; HYDROLYZE CYCLODEXTRINS (CD); LIGANDS; X-RAY STRUCTURES;

ORGANIC COMPOUNDS;

ACARBOSE; AMYLASE; CYCLODEXTRIN DERIVATIVE; GLUTAMIC ACID; LIGAND; MUTANT PROTEIN; STARCH; THERMOACTINOMYCES VULGARIS R 47 ALPHA AMYLASE 2; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; THERMOACTINOMYCES VULGARIS; X RAY DIFFRACTION;

ACARBOSE; ALPHA-AMYLASE; CATALYTIC DOMAIN; CYCLODEXTRINS; ELECTROSTATICS; LIGANDS; MICROMONOSPORACEAE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

ACTINOBACTERIA (CLASS); THERMOACTINOMYCES; THERMOACTINOMYCES VULGARIS; UNCULTURED ACTINOMYCETE;

EID: 3843112354     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M404311200     Document Type: Article

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