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Volumn 334, Issue 4, 2001, Pages 309-313
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Role of Phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by Thermoactinomyces vulgaris R-47 α-amylase 2 (TVAII). X-ray structures of the mutant TVAIIs, F286A and F286Y, and kinetic analyses of the Phe286-replaced mutant TVAIIs
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Author keywords
Amylase; Crystal structure; Cyclomaltooligosaccharide (cyclodextrin); Site directed mutagenesis
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Indexed keywords
AROMATIC COMPOUNDS;
ENZYMES;
SUBSTRATES;
SUBSTRATE RECOGNITION;
CARBOHYDRATES;
AMYLASE;
CYCLODEXTRIN;
OLIGOSACCHARIDE;
ARTICLE;
CRYSTAL STRUCTURE;
ENZYME ACTIVITY;
ENZYME KINETICS;
ENZYME MECHANISM;
MUTANT;
NONHUMAN;
PRIORITY JOURNAL;
THERMOACTINOMYCES VULGARIS;
ALANINE;
ALPHA-AMYLASE;
AMINO ACID SUBSTITUTION;
BACTERIAL PROTEINS;
BINDING SITES;
CRYSTALLOGRAPHY, X-RAY;
CYCLODEXTRINS;
KINETICS;
MICROMONOSPORACEAE;
MUTAGENESIS, SITE-DIRECTED;
PHENYLALANINE;
TYROSINE;
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EID: 0035823414
PISSN: 00086215
EISSN: None
Source Type: Journal
DOI: 10.1016/S0008-6215(01)00190-2 Document Type: Article |
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Times cited : (16)
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References (20)
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