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note
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Passive avoidance performance test was carried out according to the step-through method described by Giovannini (Behaviour Brain Research 1999, 104: 147-155). The time elapsed between the rat was placed in the light and it entered the dark compartment is recorded as Training Trial latency time (TT). Amnesia was induced in rats with scopolamine (0.5 mg/kg) given subcutaneously 1 h before Training Trial. Rats received intraperitoneally the compounds under study (4-AP, 3a, 4a, 4d) or vehicle (saline) 30 min after scopolamine treatment. Compound 1 was given 15 min before Training Trial. In a second set of experiments 1 was combined with 4-AP following the same experimental protocol. Retention trial was performed 24 h later, following a similar procedure, except that the electric shock was not given. The latency time to enter the dark compartment was measured up to a cut off time of 180 s and indicated as Retention Trial latency time (RT). Experiments were done in compliance with the recommendations of the EEC 886/609/CEE and in accordance with the national laws (DL 116/92).
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13C NMR and analytical data are reported in supplementary material.
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