Deletion of the cpku80 gene in the chestnut blight fungus, Cryphonectria parasitica, enhances gene disruption efficiency

Current Genetics

Volumn 53, Issue 1, 2008, Pages 59-66

  Lan, Xiuwan ^a,c   Yao, Ziting ^a   Zhou, Yan ^a   Shang, Jinjie ^a   Lin, Haiyan ^a   Nuss, Donald L ^b   Chen, Baoshan ^a  

^a GUANGXI UNIVERSITY   (China)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^c GUANGXI MEDICAL UNIVERSITY   (China)

Author keywords

CpKU80; Cryphonectria parasitica; Gene targeting; Homologous recombination; Nonhomologous end joining

Indexed keywords

CRYPHONECTRIA PARASITICA KU 80 PROTEIN; FUNGAL PROTEIN; MESYLIC ACID METHYL ESTER; UNCLASSIFIED DRUG;

ARTICLE; CRYPHONECTRIA PARASITICA; DELETION MUTANT; DNA REPAIR; EXPRESSED SEQUENCE TAG; FUNCTIONAL GENOMICS; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS; FUNGUS MUTANT; GENE DELETION; GENE DISRUPTION; GENE FUNCTION; GENE SEQUENCE; GENE TARGETING; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; MUTAGEN TESTING; NONHUMAN; PLANT FUNGUS INTERACTION; PRIORITY JOURNAL; WILD TYPE;

ASCOMYCOTA; CLONING, MOLECULAR; DNA REPAIR; DNA, FUNGAL; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PLANT DISEASES; SEQUENCE ANALYSIS, DNA; VIRULENCE;

CRYPHONECTRIA PARASITICA; HYPOVIRUS;

EID: 37449026653     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-007-0162-x     Document Type: Article

References (46)

1. Allen TD, Dawe AL, Nuss DL (2003) Use of cDNA microarrays to monitor transcriptional responses of the chestnut blight fungus Cryphonectria parasitica to infection by virulence-attenuating hypoviruses. Eukaryot Cell 2:1253-1265
2. Allen TD, Nuss DL (2004a) Linkage between mitochondrial hypovirulence and viral hypovirulence in the chestnut blight fungus revealed by cDNA microarray analysis. Eukaryot Cell 3:1227-1232
3. Allen TD, Nuss DL (2004b) Specific and common alterations in host gene transcript accumulation following infection of the chestnut blight fungus by mild and severe hypoviruses. J Virol 78:4145-4155
4. Anagnostakis SL (1982) Biological control of chestnut blight. Science 215:466-471
5. Aravind L, Koonin EV (2001) Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system. Genome Res 11:1365-1374
6. Chen B, Choi GH, Nuss DL (1994) Attenuation of fungal virulence by synthetic infectious hypovirus transcripts. Science 264:1762-1764
7. Chen B, Nuss DL (1999) Infectious cDNA clone of hypovirus CHV1-Euro7: A comparative virology approach to investigate virus-mediated hypovirulence of the chestnut blight fungus Cryphonectria parasitica. J Virol 73:985-992
8. Choi ES et al. (2005) Characterization of the ERK homologue CpMK2 from the chestnut blight fungus Cryphonectria parasitica. Microbiology 151:1349-1358
9. Choi GH, Nuss DL (1992) Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA. Science 257:800-803
10. Chung HJ et al. (2006) A gene encoding phosphatidyl inositol-specific phospholipase C from Cryphonectria parasitica modulates the lac1 expression. Fungal Genet Biol 43:326-336
11. Churchill ACL, Ciuffetti LM, Hansen DR, Etten HDv, Alfen NKv (1990) Transformation of the fungal pathogen Cryphonectria parasitica with a variety of heterologous plasmids. Curr Genet 17:25-31
12. Critchlow SE, Jackson SP (1998) DNA end-joining: From yeast to man. Trends Biochem Sci 23:394-398
13. Cullen D, Leong SA, Wilson LJ, Henner DJ (1987) Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene 57:21-26
14. da Silva Ferreira ME et al. (2006) The akuBKU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot Cell 5:207-211
15. Davidson RC et al. (2002) A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology 148:2607-2615
16. Dawe AL, McMains VC, Panglao M, Kasahara S, Chen B, Nuss DL (2003) An ordered collection of expressed sequences from Cryphonectria parasitica and evidence of genomic microsynteny with Neurospora crassa and Magnaporthe grisea. Microbiology 149:2373-2384
17. Deng F, Allen TD, Hillman BI, Nuss DL (2007) Comparative analysis of alterations in host phenotype and transcript accumulation following hypovirus and mycoreovirus infections of the chestnut blight fungus. Eukaryot Cell 6:1286-1298
18. Deng F, Allen TD, Nuss DL (2006) Ste12 transcription factor homologue CpST12 is down-regulated by hypovirus infection and required for virulence and female fertility of the chestnut blight fungus Cryphonectria parasitica. Eukaryot Cell 6:235-244
19. Gao S, Choi GH, Shain L, Nuss DL (1996) Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica. Appl Envir Microbiol 62:1984-1990
20. Gao S, Nuss DL (1996) Distinct roles for two G protein alpha subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption. Proc Natl Acad Sci USA 93:14122-14127
21. Goins CL, Gerik KJ, Lodge JK (2006) Improvements to gene deletion in the fungal pathogen Cryptococcus neoformans: Absence of Ku proteins increases homologous recombination, and co-transformation of independent DNA molecules allows rapid complementation of deletion phenotypes. Fungal Genet Biol 43:531-544
22. Hillman BI, Shapira R, Nuss DL (1990) Hypovirulence-associated suppression of host functions in Cryphonectria parasitica can be partially relieved by high light intensity. Phytopathology 80:950-956
23. Ishibashi K, Suzuki K, Ando Y, Takakura C, Inoue H (2006) Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora. Proc Natl Acad Sci USA 103:14871-14876
24. Jara P et al. (1996) Cloning and characterization of the eapB and eapC genes of Cryphonectria parasitica encoding two new acid proteinases, and disruption of eapC. Mol Gen Genet 250:97-105
25. Kasahara S, Nuss DL (1997) Targeted disruption of a fungal G-protein beta subunit gene results in increased vegetative growth but reduced virulence. Mol Plant Microbe Interact 10:984-993
26. Kato A, Akamatsu Y, Sakuraba Y, Inoue H (2004) The Neurospora crassa mus-19 gene is identical to the qde-3 gene, which encodes a RecQ homologue and is involved in recombination repair and postreplication repair. Curr Genet 45:37-44
27. Kazmierczak P, Kim D, Turina M, Alfen NKv (2005) A hydrophobin of the chestnut blight fungus, Cryphonectria parasitica, is required for stromal pustule eruption. Eukaryotic Cell 4:931-936
28. Kim D, Rigling D, Zhang L, Alfen NKv (1995) A new extracellular laccase of Cryphonectria parasitica is revealed by deletion of Lac1. Mol Plant-Microbe Interact 8:259-266
29. Kim M, Park S, Kim Y, Cha B, Yang M, Kim D (2004) Deletion of a hypoviral-regulated cppk1 gene in a chestnut blight fungus, Cryphonectria parasitica, results in microcolonies. Fungal Genet Biol 41:482-492
30. Krappmann S, Sasse C, Braus GH (2006) Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryot Cell 5:212-215
31. Krogh BO, Symington LS (2004) Recombination proteins in yeast. Annu Rev Genet 38:233-271
32. Lin H, Lan X, Liao H, Parsley TB, Nuss DL, Chen B (2007) Genome sequence, full-length infectious cDNA clone, and mapping of viral double-stranded RNA accumulation determinant of hypovirus CHV1-EP721. J Virol 81:1813-1820
33. Meyer V et al. (2007) Highly efficient gene targeting in the Aspergillus niger kusA mutant. J Biotechnol 128:770-775
34. Nayak T et al. (2006) A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics 172:1557-1566
35. Ninomiya Y, Suzuki K, Ishii C, Inoue H (2004) From the cover: Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proc Natl Acad Sci USA 101:12248-12253
36. Nuss DL (1992) Biological control of chestnut blight: An example of virus-mediated attenuation of fungal pathogenesis. Microbiol Rev 56:561-576
37. Nuss DL (2005) Hypovirulence: Mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632-642
38. Orbach MJ, Porro EB, Yanofsky C (1986) Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol 6:2452-2461
39. Park S, Choi E, Kim M, Cha B, Yang M, Kim D (2004) Characterization of HOG1 homologue, CpMK1, from Cryphonectria parasitica and evidence for hypovirus-mediated perturbation of its phosphorylation in response to hypertonic stress. Mol Microbiol 51:1267-1277
40. Poggeler S, Kuck U (2006) Highly efficient generation of signal transduction knockout mutants using a fungal strain deficient in the mammalian ku70 ortholog. Gene 378:1-10
41. Sambrook J, Russell DW (2001) Molecular cloning: A laboratory manual, 3rd edn. Cold Spring Harbor, New York
42. Segers GC, Regier JC, Nuss DL (2004) Evidence for a role of the regulator of G-protein signaling protein CPRGS-1 in G{alpha} subunit CPG-1-mediated regulation of fungal virulence, conidiation, and hydrophobin synthesis in the chestnut blight fungus Cryphonectria parasitica. Eukaryot Cell 3:1454-1463
43. Segers GC, van Wezel R, Zhang X, Hong Y, Nuss DL (2006) Hypovirus papain-like protease p29 suppresses RNA silencing in the natural fungal host and in a heterologous plant system. Eukaryot Cell 5:896-904
44. Takahashi T, Masuda T, Koyama Y (2006) Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae. Mol Genet Genomics 275:460-470
45. Turina M, Prodi A, Alfen NK (2003) Role of the Mf1-1 pheromone precursor gene of the filamentous ascomycete Cryphonectria parasitica. Fungal Genet Biol 40:242-251
46. van Alfen NK, Jaynes RA, Anagnostakis SL, Day PR (1975) Chestnut blight: biological control by transmissible hypovirulence in Endothia parasitica. Science USA 189:890-891