Identification of an oxidative refolding intermediate of recombinant consensus interferon from inclusion bodies and design of a two-stage strategy to promote correct disulfide-bond formation

Biotechnology and Applied Biochemistry

Volumn 48, Issue 3-4, 2007, Pages 189-198

  Liu, Yong Dong ^a   Zhang, Gui Feng ^a   Li, Jing Jing ^a   Chen, Jing ^a   Wang, Yin Jue ^a   Ding, Hong ^a   Su, Zhi Guo ^a  

^a INSTITUTE OF PROCESS ENGINEERING   (China)

Author keywords

Aggregate formation; Disulfide bond; Inclusion bodies; Intermediate; Recombinant consensus interferon; Redox system

Indexed keywords

BIOACTIVITY; CHEMICAL BONDS; ENZYME INHIBITION; OLIGOMERS; PRECIPITATION (CHEMICAL); PROTEIN FOLDING;

DISULFIDE BONDS; INCLUSION BODIES; INTRAMOLECULAR DISULFIDE BONDINGS; RECOMBINANT CONSENSUS INTERFERONS; REDOX SYSTEMS;

INTERFERONS;

ARGININE; CONSENSUS INTERFERON; CYSTEINE DERIVATIVE; DISULFIDE; OLIGOMER; PROTEIN;

ANTIVIRAL ACTIVITY; ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; CLUSTER ANALYSIS; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); DIALYSIS; DILUTION; DISULFIDE BOND; FLUORESCENCE SPECTROSCOPY; MASS; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION;

AMINO ACID SEQUENCE; ARGININE; CONSENSUS SEQUENCE; DISULFIDES; INCLUSION BODIES; INTERFERON TYPE I, RECOMBINANT; OXIDATION-REDUCTION; PEPTIDE FRAGMENTS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN RENATURATION;

EID: 37349012916     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070047     Document Type: Article

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