Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies

Protein Expression and Purification

Volumn 45, Issue 1, 2006, Pages 226-234

  Valente, C A ^a,c   Monteiro, G A ^a   Cabral, J M S ^a   Fevereiro, M ^b   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b Laboratorio Nacional de Investigacao Veterinaria   (Portugal)

^c Edificio Egas Moniz   (Portugal)

Author keywords

Biological activity; Cell disruption; Escherichia coli; Gene expression; Human interferon 2b; Inclusion bodies; Refolding; Solubilization

Indexed keywords

ALPHA2B INTERFERON; RECOMBINANT PROTEIN; UREA;

ANIMAL; ARTICLE; CELL INCLUSION; CERCOPITHECUS; CHEMISTRY; CYTOLOGY; CYTOPLASM; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; SOLUBILITY; VERO CELL;

ANIMALS; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; CYTOPLASM; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; INTERFERON ALFA-2B; RECOMBINANT PROTEINS; SOLUBILITY; UREA; VERO CELLS;

ESCHERICHIA COLI;

EID: 28844455097     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.06.014     Document Type: Article

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