Single-step Strep-tag® purification for the isolation and identification of protein complexes from mammalian cells

Proteomics

Volumn 5, Issue 5, 2005, Pages 1199-1203

  Junttila, Melissa R ^a   Saarinen, Susanna ^a   Schmidt, Thomas ^b   Kast, Juergen ^c   Westermarck, Jukka ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b IBA GmbH   (Germany)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

PR65; Protein complex purification; Protein phosphatase 2A; Strep Tactin; Strep tag

Indexed keywords

HYBRID PROTEIN; PHOSPHOPROTEIN PHOSPHATASE 2A;

AFFINITY CHROMATOGRAPHY; ARTICLE; CANCER CELL CULTURE; CELL FUNCTION; CONTROLLED STUDY; ELUTION; HUMAN; HUMAN CELL; MAMMAL CELL; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE, TUMOR; CHROMATOGRAPHY, AFFINITY; HUMANS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS;

MAMMALIA;

EID: 17044415362     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200400991     Document Type: Article

References (10)

1. Aebersold, R., Mann, M., Nature 2003, 422, 198-207.
2. Dziembowski, A., Seraphin, B., FEBS Lett. 2004, 556, 1-6.
3. Puig, O., Caspary, F., Rigaut, G., Rutz, B. et al., Methods 2001, 24, 218-229.
4. Westermarck, J., Weiss, C., Saffrich, J., Kast, J. et al., EMBO J. 2002, 21, 451-460.
5. Skerra, A., Schmidt, T. G., Methods Enzymol. 2000, 326, 271-304.
6. Schmidt, T. G., Koepke, J., Frank, R., Skerra, A., J. Mol. Biol. 1996, 255, 753-766.
7. Janssens, V., Goris, J., Biochem. J. 2001, 353, 417-439.
8. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.
9. Le Blanc, J. C., Hager, J. W., Ilisiu, A. M., Hunter, C. et al., Proteomics 2003, 3, 859-869.
10. Gingras, A. C., Raught, B., Sonenberg, N., Annu Rev. Biochem. 1999, 68, 913-963.