Flow cytometric quantitation of opsonophagocytosis and intracellular killing of Candida albicans using a whole blood microassay

Cytometry Part A

Volumn 71, Issue 12, 2007, Pages 1027-1033

  Pattanapanyasat, Kovit ^a   Sukapirom, Kasama ^a   Tachavanich, Kalaya ^a   Kaewmoon, Sakorn ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Bis carboxyethyl carboxyfluorescein pentaacetoxymethylester; Candida albicans; Flow cytometry; Phagocytes; Phagocytosis; Polymorphonuclear leucocytes; Thalassemia

Indexed keywords

ARTICLE; BLOOD ANALYSIS; CANDIDA ALBICANS; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; HUMAN; INTRACELLULAR KILLING; NEUTROPHIL; PHAGOCYTE; PHAGOCYTOSIS; PRIORITY JOURNAL; THALASSEMIA; YEAST CELL;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD13; BETA-THALASSEMIA; CANDIDA ALBICANS; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMANS; NEUTROPHILS; OPSONIN PROTEINS; PHAGOCYTOSIS;

EID: 36849010134     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20475     Document Type: Article

References (26)

1. Bass DA, Pearce JW, Dechaletet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J Immunol 1983;130:1910-1917.
2. Martin E, Bhakdi S. Quantitative analysis of opsonophagocytosis and of killing of Candida albicans by human peripheral blood leukocytes by using flow cytometry. J Clin Microbiol 1991;29:2013-2023.
3. Lehmann AK, Sørnes S, Halstensen A. Phagocytosis: Measurement by flow cytometry. J Immunol Methods 2000;243:229-242.
4. Perticarari S, Presani G, Banfi E. A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood. J Immunol Methods 1994;170:117-124.
5. Caldefie-Chézet F, Walrand S, Moinard C, Tridon A, Chassagne J, Vasson M-P. Is the neutrophil reactive oxygen species production measured by luminal and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction. Clin Chim Acta 2002;319:9-17.
6. White-Owen C, Alexander JW, Sramkoski RM, Babcock GF. Rapid whole-blood microassay using flow cytometry for measuring neutrophil phagocytosis. J Clin Microbiol 1992;30:2071-2076.
7. Vuorte J, Jansson S-E, Repo H. Evaluation of red blood cell lysing solutions in study of neutrophil oxidative burst by the DCFH assay. Cytometry 2001;43:290-296.
8. Van Eeden SF, Klut MWE, Walker BAM, Hogg JC. The use of flow cytometry to measure neutrophil function. J Immunol Methods 1999;232:23-43.
9. Lehrer RI. Measurement of candidacidal activity of specific leukocyte types in mixed cell populations. I. Normal, myeloperoxidase-deficient, and chronic granulomatous disease neutrophils. Infect Immun 1970;2:42-47.
10. Bhakdi S, Greulich S, Muhly M, Eberspacher B, Becker H, Thiele A, Hugo F. Potent leukocidal action of Escherichia coli hemolysis mediated by permeabilization of target cell membranes. J Exp Med 1989;169:838-854.
11. Dunn PA, Tyrer HW. Quantitation of neutrophil phagocytosis, using fluorescent latex beads. J Lab Clin Med 1981;98:374-381.
12. Haslett C, Guthrie LA, Kopaniak MM, Johnston RB, Henson PM. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide. Am J Pathol 1985;119:101-110.
13. Repo H, Jansson S-E, Leirisalo-Repo M. Flow cytometric determination of CD11b upregulation in vivo. J Immunol Methods 1993;164:193-202.
14. Bass DA, Olbrantz P, Szejda P, Seeds MC, McCall CE. Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection. J Immunol 1986;136:860-866.
15. Chollet-Martin S, Montravers P, Gilbert C, Elbim C, Desmonts JM, Fagon JY, Gougerot-Pocidalo MA. Subpopulation of hyperresponsive polymorphonuclear neutrophils in patients with adult respiratory distress syndrome. Am Rev Respir Dis 1992;146:990-996.
16. Cantinieaux B, Hariga C, Ferster A, de Maertelaere E, Toppet M, Fondu P. Neutrophil dysfunctions in thalassemia major: The role of cell iron overload. Eur J Haematol 1987;39:28-34.
17. Speer ChP, Gahr M, Schuff-Werner P, Schroter W. Immunologic evaluation of children with homozygous β-thalassemia treated with desferioxamine. Acta Haematol 1990;83:76-81.
18. Giumtoli JMT, Estevez ME, Sen L, Penalver JA. Defective function of the peripheral blood neutrophils in thalassaemia major. Am J Pediatr Hematol Oncol 1984;4:215-217.
19. Ballart IJ, Estevez ME, Sen L, Diez RA, Giuntoli J, de Miani SA, Penalver J. Progressive dysfunction of monocytes associated with iron overload and age in patients with thalassemia major. Blood 1986;67:105-109.
20. Skoutelis ATH, Lianou E, Papavassiliou TH, Karamerou A, Politi K, Bassaris HP. Defective phagocytic and bactericidal functions of polymorphonuclear leucocytes in patients with β thalassaemia major. J Infect 1984;8:118-122.
21. Khan AJ, Lee CK, Wolff JA, Chang H, Khan P, Evans HE. Defects of neutrophil chemotaxis and random migration in thalassemia major. Pediatrics 1977;60:349-351.
22. Khalifa AS, Fattah SA, Maged Z, Sabry F, Mohamed HA. Immunoglobulin levels, opsonic activity and phagocytic power in Egyptian thalassemic children. Acta Haematol 1983;69:136-139.
23. Abbas AK, Lichtman AH, Pober JS. Cellular and Molecular Immunology, 4th ed. Philadelphia: W.B. Saunders; 2000. 553 p.
24. Busetto S, Trevisan E, Patriarca P, Menegazzi R. A single-step, sensitive flow cytofluorometric assay for the simultaneous assessment of membrane-bound and ingested Candida albicans in phagocytosing neutrophils. Cytometry Part A 2004;58A:201-206.
25. Fattorossi A, Nisini R, Pizzolo JG, D'Amelio R. New, simple flow cytometry technique to discriminate between internalized and membrane-bound particles in phagocytosis. Cytometry 1989;10:320-325.
26. Saresella M, Roda K, Speciale L, Taramelli D, Mendozzi E, Guerini F, Ferrante P. A rapid evaluation of phagocytosis and killing of Candida albicans by CD13+ leukocytes. J Immunol Methods 1997;210:227-234.