Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction

Clinica Chimica Acta

Volumn 319, Issue 1, 2002, Pages 9-17

  Caldefie Chézet, Florence ^a   Walrand, Stephane ^a   Moinard, Christophe ^a,b   Tridon, Arlette ^a   Chassagne, Jacques ^c   Vasson, Marie Paule ^a,c  

^a UNIVERSITÉ CLERMONT AUVERGNE   (France)

^b Faculte de Pharmacie   (France)

^c Departement d'Anatomopathologie   (France)

Author keywords

Chemiluminescence; Cytochrome c; Flow cytometry; Polymorphonuclear neutrophils; Reactive oxygen species

Indexed keywords

ACETOACETIC ACID DERIVATIVE; CYTOCHROME C; DICHLORODIHYDROFLUORESCEIN DIACETATE; LUCIGENIN; LUMINOL; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHEMICAL ANALYSIS; CHEMOLUMINESCENCE; CONTROLLED STUDY; COST; EXTRACELLULAR SPACE; FLOW CYTOMETRY; INTERMETHOD COMPARISON; MALE; MOLECULAR PROBE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RAT; REDUCTION; REPRODUCIBILITY;

ACRIDINES; ANIMALS; CHEMILUMINESCENT MEASUREMENTS; CYTOCHROME C GROUP; EXTRACELLULAR SPACE; FLOW CYTOMETRY; FLUORESCEINS; INTRACELLULAR FLUID; KINETICS; LUMINOL; MALE; NEUTROPHILS; OXIDATION-REDUCTION; RATS; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; SENSITIVITY AND SPECIFICITY;

EID: 0037035494     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(02)00015-3     Document Type: Article

References (27)

1. The respiratory burst of neutrophils and its deficiency
2. Relationship between intracellulary and extracellulary generated oxygen metabolites from primed polymorphonuclear leukocytes differs from that obtained from non-primed cells
3. Intra and extracellular events in luminol dependent chemiluminescence of polymorphonuclear leukocytes
4. The superoxide-generating oxidase of phagocytic cells
5. Effect of the protein kinase C inhibitor GF 109 203 X on elastase release and respiratory burst of human neutrophils
6. Etude de la physiopathologie des mécanismes d'activation des neutrophiles: Une voie d'avenir?
7. Neutrophil stimulation: Receptor, membrane, and metabolic events
8. Molecular basis of activation and regulation of the phagocyte respiratory burst
9. Bactericidal activity of a superoxide anion-generating system
10. Réactions de luminescence
11. The respiratory burst of phagocytes
12. Phosphorylation of the respiratory burst oxidase subunit p67phox during human neutrophil activation
13. Hydrogen peroxide as a potent bacteriostatic antibiotic: Implications for host defense
14. Luminol dependent chemiluminescence microassay for phagocytic function
15. Measurement of human neutrophil respiratory burst activity during phagocytosis of bacteria
16. Further characterization of the neutrophil oxidative burst by flow cytometry
17. Leptin: A potential regulator of polymorphonuclear neutrophil bactericidal action?
18. Aging: A barrier to renutrition? Nutritional and immunologic evidence in rats
19. Effect of alpha-1 acid glycoprotein on human polymorphonuclear neutrophils: Influence of glycan microheterogeneity
20. Glutamine utilization by rat neutrophils
21. Flow cytometry analysis of respiratory burst activity in phagocytes with hydroethidine and 2′-7′-dichlorofluorescin
22. Subpopulations of neutrophils with increased oxidative product formation in blood of patients with infection
23. Myeloperoxidase-based chemiluminescence of polymorphonuclear leukocytes and monocytes
24. Comparison of circulating phagocyte oxidative activity measured by chemiluminescence in whole blood and isolated polymorphonuclear leukocytes
25. Improved preparation of leukocytes for chemiluminescent study of human phagocytic leukocyte-generated reactive oxygen species
26. Whole blood luminol enhanced chemiluminescence: Statistical analysis of the responses of different subjects
27. What do we measure by a luminol-dependent chemiluminescence of phagocytes?