The use of flow cytometry to measure neutrophil function

Journal of Immunological Methods

Volumn 232, Issue 1-2, 1999, Pages 23-43

  Van Eeden, Stephan F ^a   Klut, Maria E ^a   Walker, Blair A M ^a   Hogg, James C ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Adhesion; Calcium; Degranulation; F actin; Myeloperoxidase; Phagocytosis; Polymorphonuclear leukocytes; Reactive oxygen species

Indexed keywords

REACTIVE OXYGEN METABOLITE;

CALCIUM MOBILIZATION; CELL ADHESION; CELL AGGREGATION; CELL FUNCTION; DEGRANULATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEASUREMENT; NEUTROPHIL; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; REVIEW;

EID: 0033397444     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00148-9     Document Type: Article

References (183)

1. 2+ stores by cytochalasins. Exp. Cell Res. 234:1997;379.
2. Anderson R., Mahomed A.G. Calcium efflux and influx in f-Met-Leu-Phe (fMLP)-activated human neutrophils are chronologically distinct events. Clin. Exp. Immunol. 110:1997;132.
3. Anderson D.C., Springer T.A. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins. Annu. Rev. Med. 38:1987;175.
4. 2+ influx across the plasma membrane of human neutrophils. Mol. Pharmacol. 30:1986;437.
5. Antal P., Sipka S., Suranyi P., Csipo I., Seres T., Marodi L., Szegedi G. Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake. Ann. Hematol. 70:1995;259.
6. Arends M.J., Morris R.G., Wyllie A.H. Apoptosis. The role of the endonuclease. Am. J. Pathol. 136:1990;593.
7. Arnaout M.A. Structure and function of the cell adhesion molecule CD11/CD18. Blood. 75:1990;1037.
8. Arnaout M.A., Hakim R.M., Todd R.F. III, Dana N., Colten H.R. Increased expression of an adhesion-promoting surface glycoprotein in the granulocytopenia of hemodialysis. N. Engl. J. Med. 312:1985;457.
9. Babior B.M., Kipnes R.S., Curnutte J.J. Biological defense mechanism. The production by leukocytes of superoxide a potential bactericidal agent. J. Clin. Invest. 52:1970;791.
10. Bainton, D.F., 1992. Development biology of neutrophils and eosinophils. In: Gallin, J.I., Goldstein, I.M., Snyderman, R. (Eds.), Inflammation: Basic Principles and Clinical Correlates, Chap. 17, 2nd edn. Raven Press, New York, p. 303.
11. Bainton D.F., Miller L.J., Kishimoto T.K., Springer T.A. Leukocyte adhesion receptors are stored in peroxidase-negative granules of human neutrophils. J. Exp. Med. 166:1997;1641.
12. Bass D.A., Parce J.W., Dechatelet L.R., Szejda P., Seeds M.C., Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130:1983;1910.
13. Bassoe C.F. Processing of staphylococcus aureus and zymosan particles by human leukocytes measured by flow cytometry. Cytometry. 5:1984;86.
14. Bassoe C.F., Solberg C.O. Phagocytosis of Staphylococcus aureus by human leukocytes: quantitation by a flow cytometric and a microbiological method. Acta Pathol. Microbiol. Immunol. Scand. 92:1984;43.
15. Bateman J., Parida S.K., Nash G.B. Neutrophil assay for clinical studies. Cell Biochem. Funct. 11:1993;87.
16. 2+ regulates the respiratory burst of human neutrophils. Biochem. Biophys. Acta. 1404:1998;475.
17. Bengtsson T., Jaconi M.E., Gustafson M., Magnusson K.-E., Theler J.-M., Lew D.P., Stendahl O. Actin dynamics in human neutrophils during adhesion and phagocytosis is controlled by changes in intracellular free calcium. Eur. J. Cell Biol. 62:1993;49.
18. Bevilacqua M.P. Endothelial-leukocyte adhesion molecules. Annu. Rev. Immunol. 11:1993;767.
19. Bicknell S., van Eeden S., Hayashi S., Hards J., English D., Hogg J.C. A non-radioisotopic method for tracing neutrophils in vivo using 5′-bromo-2′-deoxyuridine. Am. J. Respir. Cell Mol. Biol. 10:1994;16.
20. Bjerknes R., Bassoe C.F. Phagocyte C3-mediated attachment and internalization: flow cytometric studies using a fluorescence quenching technique. Blut. 49:1984;315.
21. Bohmer R.H., Trinkle L.S., Staneck J.L. Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxidative burst. Cytometry. 13:1992;525.
22. Borregaard N., Heiple J.M., Simons E.R., Clark R.A. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation. J. Cell Biol. 97:1983;52.
23. 2 formation of biological systems. Anal. Biochem. 80:1977;145.
24. Brach M.A., deVos S., Gruss H.J., Herrmann F. Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death. Blood. 80:1992;2920.
25. Burton J.L., Kehrli M.E. Jr., Kapil S., Horst R.L. Regulation of L-selectin and CD18 on bovine neutrophils by glucocorticoids: effects of cortisol and dexamethasone. J. Leukocyte Biol. 57:1995;317.
26. Carlos T.M., Harlan J.M. Leukocyte-endothelial adhesion molecules. Blood. 84:1994;2068.
27. Carulli G. Applications of flow cytometry in the study of human neutrophil biology and pathology. Hematopathol. Mol. Hematol. 10:1996;39.
28. Carulli G., Sbrana S., Minnucci S., Azzara A., Angiolini C., Gullaci A.R., Ambrogi F. Actin polymerization in neutrophils from patients affected by Myelodysplastic syndromes - a flow cytometric stud. Leuk. Res. 21:1997;513.
29. Chacon-Cruz E., Oelberg D.G., Davis P., Buescher E.S. Membrane depolarization and depletion of intracellular calcium stores are associated with delay of apoptosis in human neutrophils. J. Leukocyte Biol. 64:1998;759.
30. Champagne C.M., Vaikuntam J., Warbington M.L., Rose L., Daniel M.A., van Dyke T.E. Cytoskeletal actin reorganization in neutrophils from patients with localized juvenile periodontitis. J. Periodontol. 69:1998;209.
31. Chen W.-Y., Lei H.-Y., Wang J.-Y., Lu C.-C. Direct measurement of neutrophil F-actin content in microvolume whole blood samples. Int. Arch. Allergy Immunol. 110:1996;325.
32. Colotta F., Re F., Polentarutti N., Sozzani S., Mantovani A. Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products. Blood. 80:1992;2012.
33. Condliffe A.M., Chilvers E.R., Haslett C., Dransfield I. Priming differentially regulates neutrophil adhesion molecule expression/function. Immunology. 89(1):1996;105.
34. Condliffe A.M., Kitchen E., Chilvers E.R. Neutrophil priming: pathophysiological consequences and underlying mechanisms. Clin. Sci. 94:1998;461.
35. Condliffe A.M., Hawkins P.T., Stephens L.R., Haslett C., Chilvers E.R. Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation. FEBS Lett. 439:1998;147.
36. Conklyn M.J., Neote K., Showell H.J. Chemokine-dependent upregulation of CD11b on specific leukocyte subpopulations in human whole blood: effect of anticoagulant on rantes and MIP-1 beta stimulation. Cytokine. 8:1996;762.
37. Craddock P.R., Hammerschmidt D., White J.G., Dalmosso A.P., Jacob H.S. Complement (C5a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia. J. Clin. Invest. 60:1977;260.
38. Diaz-Gonzalez F., Gonzalez-Alvaro I., Campanero M.R., Mollinedo F., del Pozo M.A., Munoz C., Pivel J.P., Sanchez-Madrid F. Prevention of in vitro neutrophil-endothelial attachment through shedding of L-selectin by nonsteroidal antiinflammatory drugs. J. Clin. Invest. 95:1995;1756.
39. Doerfler M.E., Danner R.L., Shelhamer J.H., Parrillo J.E. Bacterial lipopolysaccharides prime human neutrophils for enhanced production of leukotriene B4. J. Clin. Invest. 83(3):1989;970.
40. Doerfler M.E., Weiss J., Clark J.D., Elsbach P. Bacterial lipopolysaccharide primes human neutrophils for enhanced release of arachidonic acid and causes phosphorylation of an 85-kDa cytosolic phospholipase A2. J. Clin. Invest. 93:1994;1583.
41. Doerschuk C.M., Allard M.F., Hogg J.C. Neutrophil kinetics in rabbits during infusion of zymosan-activated plasma. J. Appl. Physiol. 67:1989;88.
42. Doerschuk C.M., Beyers N., Coxson H.O., Wiggs B., Hogg J.C. Comparison of neutrophil and capillary diameters and their relation to neutrophil sequestration in the lung. J. Appl. Physiol. 74:1993;3040.
43. Duque R.E., Ward P.A. Quantitative assessment of neutrophil function by flow cytometry. Anal. Quant. Cytol. Histol. 9:1987;42.
44. Durack G., Lawler G., Kelley S., Ragheb K., Roth R.A., Ganey P., Robinson J.P. Time interval gating for analysis of cell function using flow cytometry. Cytometry. 12:1991;8701.
45. Elsner J., Kaever V., Emmendorffer A., Breidenbach T., Lohmann-Mattes M.-L., Roesler J. Heterogeneity in the mobilization of cytoplasmic calcium by human polymorphonuclear leukocytes in response to fMLP, C5a and IL-8/NAP-1. J. Leukocyte Biol. 51:1992;77.
46. Emmendorffer A., Hecht M., Lohmann-Matthes M.L., Roesler J. A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123. J. Immunol. Methods. 131:1990;269.
47. Epling C.L., Stites D.P., McHugh T.M., Chong H.O., Blackwood L.L., Wara D.W. Neutrophil function screening in patients with chronic granulomatous disease by a flow cytometric method. Cytometry. 13:1992;615.
48. Fattorossi A., Nisini R., Pizzolo J.G., D'Amelio R. New, simple flow cytometry technique to discriminate between internalized and membrane-bound particles in phagocytosis. Cytometry. 10:1989;320.
49. Fernandez-Segura, E., Garcia, J.M., Santor, J.L., Campos, A., 1995. Shape, F-actin and surface morphology changes during chemotactic peptide-induced polarity in human neutrophils, 241, 519.
50. Fittschen C., Sandhaus R.A., Worthen G.S., Henson P.M. Bacterial lipopolysaccharide enhances chemoattractant-induced elastase secretion by human neutrophils. J. Leukocyte Biol. 43:1988;547.
51. Froelich-Ammon S.J., Patchan M.W., Osheroff N., Thompson R.B. Topoisomerase II binds to ellipticine in the absence or presence of DNA. Characterization of enzyme-drug interactions by fluorescence spectroscopy. J. Biol. Chem. 270:1995;14998.
52. Gallin, J.I., 1992. Phagocytic cells: disorders of function. In: Gallin, J.I., Goldstein, I.M., Snyderman, R. (Eds.), Inflammation: Basic Principles and Clinical Correlates. Raven Press, New York, p. 859.
53. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:1992;493.
54. Gong J., Traganos F., Darzynkiewicz Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal. Biochem. 218:1994;314.
55. Gorman A.M., Samali A., McGowan A.J., Cotter T.G. Use of flow cytometry techniques in studying mechanisms of apoptosis in leukemic cells. Cytometry. 29:1997;97.
56. Grasl-Kraupp B., Ruttkay-Nedecky B., Koudelka H., Bukowska K., Bursch W., Schulte-Hermann R. In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note. Hepatology. 21:1995;1465.
57. Guthrie L.A., McPhail L.C., Henson P.M., Johnston R.B. Jr. Priming of neutrophils for enhanced release of oxygen metabolites by bacterial lipopolysaccharide. Evidence for increased activity of the superoxide-producing enzyme. J. Exp. Med. 160:1984;1656.
58. Guyer D.A., Moore K.L., Lynam E.B., Schammel C.M., Rogelj S., McEver R.P., Sklar L.A. P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation. Blood. 88:1996;2415.
59. Hansen P.R. Myocardial reperfusion injury: experimental evidence and clinical relevance. Eur. Heart J. 16:1995;734.
60. Haslett C., Guthrie L.A., Kopaniak M.M., Johnston R.B. Jr., Henson P.M. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide. Am. J. Pathol. 119:1985;101.
61. Hasui M., Hirabayashi Y., Kobayashi Y. Simultaneous measurement by flow cytometry of phagocytosis and hydrogen peroxide production of neutrophils in whole blood. J. Immunol. Methods. 117:1989;53.
62. Haynes A.P., Fletcher J., Garnett M., Robins A. A novel flow cytometric method for measuring protein digestion within the phagocytic vacuole of polymorphonuclear neutrophils. J. Immunol. Methods. 135:1990;155.
63. Hernandez L.A., Grisham M.B., Twohig B., Arfors K.E., Harlan J.M., Granger D.N. Role of neutrophils in ischemia-reperfusion-induced microvascular injury. Am. J. Physiol. 253:1987;H699.
64. Hewitt J., Reardon D.M. The measurement of neutrophil myeloperoxidase by flow cytochemistry is influenced by the pseudoperoxidase activity of hemoglobin. Blood. 78:1991;1891.
65. Hogg J.C. Neutrophil kinetics and lung injury. Physiol. Rev. 67:1987;249.
66. Hogg N., Stewart M.P., Scarth S.L., Newton R., Shaw J.M., Law S.K., Klein N. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1. J. Clin. Invest. 103:1999;97.
67. Horl W.H., Schafer R.M., Horl M., Heidland A. Neutrophil activation in acute renal failure and sepsis. Arch. Surg. 125:1990;651.
68. Hoy T.G. Flow cytometry: clinical applications in haematology. Ballieres Clin. Haematol. 3:1990;977.
69. Jones J., Morgan B.P. Apoptosis is associated with reduced expression of complement regulatory molecules, adhesion molecules and other receptors on polymorphonuclear leucocytes: functional relevance and role in inflammation. Immunology. 86:1995;651-660.
70. Justement L.B., Cambier J.C., Rijkers G.T., Fittschen C. Use of flow cytometry to study ion transport in lymphocytes and granulocytes. Cell Biol. 9:1990;353.
71. Kelley K.A. Very early detection of changes associated with cellular activation using a modified flow cytometer. Cytometry. 12:1991;464.
72. Kishimoto T.K., Jutila M.A., Berg E.L., Butcher E.C. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science. 245:1989;1238.
73. Kitagawa Y., van Eeden S.F., Redenbach D.M., Daya M., Walker B.A., Klut M.E., Wiggs B.R., Hogg J.C. Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. J. Appl. Physiol. 82:1997;1397. [see comments].
74. Klut M.E., Whalen B.A., Hogg J.C. Activation-associated changes in blood and bone marrow neutrophils. J. Leukocyte Biol. 62:1997;187.
75. Konstantopoulos K., Grotta J.C., Sills C., Wu K.K., Hellums J.D. Shear-induced platelet aggregation in normal subjects and stroke patients. Thromb. Haemostasis. 74:1995;1329.
76. Konstantopoulos K., Neelamegham S., Burns A.R., Hentzen E., Kansas G.S., Snapp K.R., Berg E.L., Hellums J.D., Smith C.W., McIntire L.V., Simon S.I. Venous levels of shear support neutrophil-platelet adhesion and neutrophil aggregation in blood via P-selectin and beta2-integrin. Circulation. 98:1998;873.
77. Koopman G., Reutelingsperger C.P., Kuijten G.A., Keehnen R.M., Pals S.T., van Oers M.H. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 84:1994;1415.
78. Korlipara L.V., Leon M.P., Rix D.A., Douglas M.S., Gibbs P., Bassendine M.F., Kirby J.A. Development of a flow cytometric assay to quantify lymphocyte adhesion to cytokine-stimulated human endothelial and biliary epithelial cells. J. Immunol. Methods. 191:1996;121.
79. Kuijpers T.W., Tool A.T., van der Schoot C.E., Ginsel L.A., Onderwater J.J., Roos D., Verhoeven A.J. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood. 78:1991;1105.
80. Kuijpers T.W., van Lier R.A., Hamann D., de Boer M., Thung L.Y., Weening R.S., Verhoeven A.J., Roos D. Leukocyte adhesion deficiency type 1 (LAD-1)/variant. A novel immunodeficiency syndrome characterized by dysfunctional beta2 integrins. J. Clin. Invest. 100:1997;1725.
81. Larson R.S., Springer T.A. Structure and function of leukocyte integrins. Immunol. Rev. 114:1990;181.
82. Lawrence E., van Eeden S.F., English D., Hogg J.C. Polymorphonuclear leukocyte migration of Streptococcal pneumonia: evidence for slow migration of cells recently released from the bone marrow. Am. J. Respir. Cell Mol. Biol. 16:1996;217.
83. Lee A., Young S.K., Henson P.M., Haslett C. Modulation of neutrophil programmed cell death by inflammatory mediators. FASEB J. 3:1989;A1344.
84. Lew D.P. Receptor signalling and intracellular calcium in neutrophil activation. Eur. J. Clin. Invest. 19:1989;338.
85. Liles W.C., Klebanoff S.J. Regulation of apoptosis in neutrophils - Fas track to death? J. Immunol. 155:1995;3289.
86. Liles W.C., Ledbetter J.A., Waltersdorph A.W., Klebanoff S.J. Cross-linking of CD18 primes human neutrophils for activation of the respiratory burst in response to specific stimuli: implications for adhesion-dependent physiological responses in neutrophils. J. Leukocyte Biol. 58:1995;690.
87. Lofgren R., Ng-Sikorski J., Sjolander A., Andersson T. β2 integrin engagement triggers actin polymerization and phosphatidylinositol trisphosphate formation in non-adherent human neutrophils. J. Cell Biol. 6:1993;1597.
88. Lopez M., Olive D., Mannoni P. Analysis of cytosolic ionized calcium variation in morphonuclear leukocytes using flow cytometry and Indo-1 AM. Cytometry. 10:1989;165.
89. Lund-Johansen F., Terstappen L.W. Differential surface expression of cell adhesion molecules during granulocyte maturation. J. Leukocyte Biol. 54:1993;47.
90. Macey M.G., McCarthy D.A., Howells G.L., Curtis M.A., King G., Newland A.C. Multiparameter flow cytometric analysis of polymorphonuclear leucocytes in whole blood from patients with adult rapidly progressive periodontitis reveals low expression of the adhesion molecule L-selectin (Cd62L). Cytometry. 34:1998;152.
91. Maftah A., Huet O., Gallet P.-F., Ratinaud M.-H. Flow cytometry's contribution to the measurement of cell functions. Cell. 78:1993;85.
92. Majeed M., Perskvist N., Ernst J.E., Orselius K., Stendahl O. Roles of calcium and annexins in phagocytosis and elimination of an attenuated strain of Mycobacterium tuberculosis in human neutrophils. Microb. Pathog. 24:1998;309.
93. Malech H.L., Gallin J.I. Current concepts: immunology. Neutrophils in human diseases. N. Engl. J. Med. 317:1987;687.
94. Maloney M.A., Patt H.M. Granulocyte transit from bone marrow to blood. Blood. 31(2):1968;195.
95. Martin S.J., Reutelingsperger C.P., McGahon A.J., Rader J.A., van Schie R.C., LaFace D.M., Green D.R. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med. 182:1995;1545.
96. Marshall C., Gray L., Lambert D., Shenton B., Plusa S.M., Kirby J.A. A novel flow-cytometric assay to assess neutrophil adhesion in whole blood. Biochem. Soc. Trans. 25:1997;740.
97. Matsuba K.T., van Eeden S.F., Bicknell S.G., Walker B.A., Hayashi S., Hogg J.C. Apoptosis in circulating PMN: increased susceptibility in L-selectin-deficient PMN. Am. J. Phys. 272:1997;H2852.
98. Mazzone A., de Servi S., Mazzucchelli I., Fossati G., Gritti D., Canale C., Cusa C., Ricevuti G. Increased expression of CD11b/CD18 on phagocytes in ischaemic disease: a bridge between inflammation and coagulation. Eur. J. Clin. Invest. 27:1997;648.
99. McAfee J.G., Thakur M.L. Survey of radioactive agents for in vitro labeling of phagocytic leukocytes: I. Soluble agents. J. Nucl. Med. 17:1976;480.
100. McColl S.R., Naccache P.H. Calcium mobilization assays. Methods Enzymol. 288:1997;301.
101. McCord J.M., Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244:1969;6049.
102. Merry C., Puri P., Reen D.J. Phosphorylation and the actin cytoskeleton in defective newborn neutrophile chemotaxis. Pediatr. Res. 44:1998;259.
103. Mesner, P.W., Kaufman, S.H., 1997. Methods utilized in the study of apoptosis. In: Kaufman, S.H. (Ed.), Apoptosis; Pharmacological implications and Therapeutic Opportunities, Vol 41. Academic Press, New York, pp. 57-81.
104. Miyashita T., Reed J.C. Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell. 80:1995;293-299.
105. Nakagawa M., Terashima T., D'yachkova Y., Bondy G.P., Hogg J.C., van Eeden S.F. Glucocorticoid-induced granulocytosis: contribution of marrow release and demargination of intravascular granulocytes. Circulation. 98:1998;2307.
106. Nakagawa M., Bondy G.P., Waisman M.D., Minshall D., Hogg J.C., van Eeden S.F. The effect of glucocorticoids on the expression of L-selectin on polymorphonuclear leukocytes. Blood. 93:1999;2730.
107. Neelamegham S., Taylor A.D., Hellums J.D., Dembo M., Smith C.W., Simon S.I. Modeling the reversible kinetics of neutrophil aggregation under hydrodynamic shear. Biophys. J. 72:1997;1527.
108. Neelamegham S., Taylor A.D., Burns A.R., Smith C.W., Simon S.I. Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1. Blood. 92:1998;1626.
109. Neumann F.J., Marx N., Gawaz M., Brand K., Ott I., Rokitta C., Sticherling C., Meinl C., May A., Schomig A. Induction of cytokine expression in leukocytes by binding of thrombin-stimulated platelets. Circulation. 95:1997;2387.
110. Novak E.J., Rabinovitch P.S. Improved sensitivity in flow cytometric intracellular ionized calcium measurement using Fluo-3/Fura red fluorescence ratios. Cytometry. 17:1994;135.
111. O'Brien M.C., Bolton W.E. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry. 19:1995;243-255.
112. Omann G.M., Harter J.M. Pertussis toxin effects on chemoattractant-induced response heterogeneity in human PMNs utilizing Fluo-3 and flow cytometry. Cytometry. 12:1991;252.
113. Orfao A., Ruiz-Arguelles A. General concepts about cell sorting techniques. Clin. Biochem. 29:1996;5.
114. Packman C.H., Lichtman M.A. Activation of neutrophils, measurement of actin conformational changes by flow cytometry. Blood Cells. 16:1990;193.
115. Parsons P.E., Worthen G.S., Moore E.E., Tate R.M., Henson P.M. The association of circulating endotoxin with the development of the adult respiratory distress syndrome. Am. Rev. Respir. Dis. 140:1989;294.
116. Petrequin P.R., Todd R.F., Devall L.J., Boxer L.A., Curnutte J.T. Association between gelatinase release and increase plasma membrane expression of the Mo1 glycoprotein. Blood. 69:1987;605.
117. Perticarari S., Presani G., Mangiarotti M.A., Banfi E. Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils. Cytometry. 12:1991;687.
118. Peters M.J., Heyderman R.S., Hatch D.J., Klein N.J. Investigation of platelet-neutrophil interactions in whole blood by flow cytometry. J. Immunol. Methods. 209:1997;125.
119. 2+ signalling events in neutrophils detected by rapid confocal laser scanning. Biochem. J. 310:1995;445.
120. Pinsky M.R., Vincent J.L., Deviere J., Alegre M., Kahn R.J., Dupont E. Serum cytokine levels in human septic shock. Relation to multiple-system organ failure and mortality. Chest. 103(2):1993;565.
121. Repo H., Jansson S.E., Leirisalo-Repo M. Flow cytometric determination of CD11b upregulation in vivo. J. Immunol. Methods. 164:1993;193.
122. Repo H., Jansson S.E., Leirisalo-Repo M. Anticoagulant selection influences flow cytometric determination of CD11b upregulation in vivo and ex vivo. J. Immunol. Methods. 185:1995;65.
123. Ricevuti G. Host tissue damage by phagocytes. Ann. N. Y. Acad. Sci. 832:1997;426.
124. Richardson M.P., Ayliffe M.J., Helbert M., Davies E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J. Immunol. Methods. 219:1998;187.
125. 1. Cytometry. 11:1990;923.
126. Ringertz B., Palmblad J., Lindgren J.A. Stimulus-specific neutrophil aggregation: evaluation of possible mechanisms for the stimulus-response apparatus. J. Lab. Clin. Med. 106:1985;132.
127. Rochon Y.P., Frojmovic M.M. Dynamics of human neutrophil aggregation evaluated by flow cytometry. J. Leukocyte Biol. 50:1991;434.
128. Roesler J., Hecht M., Freihorst J., Lohmann-Matthes M.L., Emmendorffer A. Diagnosis of chronic granulomatous disease and its mode of inheritance by dihydrorhodamine 123 and flow cytometry. Eur. J. Pediatr. 150:1990;161.
129. 2. J. Clin. Invest. 60:1977;1266.
130. Rothe G., Emmendorffer A., Oser A., Roesler J., Valet G. Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123. J. Immunol. Methods. 138:1991;133.
131. Rotrosen D., Gallin J.I., Spiegel A.M., Malech H.L. Subcellular localization of Gi alpha in human neutrophils. J. Biol. Chem. 263:1988;10958.
132. Santos J.L., Montes M.J., Gutierrez F., Ruiz C. Evaluation of phagocytic capacity with a modified flow cytometry technique. Immunol. Lett. 45:1995;1.
133. Saresella M., Roda K., Speciale L., Taramelli D., Mendozzi E., Guerini F., Ferrante P. A flow cytometric method for the analysis of phagocytosis and killing by polymorphonuclear leukocytes. Ann. N. Y. Acad. Sci. 832:1997;53.
134. Savill J.S., Henson P.M., Haslett C. Phagocytosis of aged human neutrophils by macrophages is mediated by a novel "charge-sensitive" recognition mechanism. J. Clin. Invest. 84(5):1989;1518.
135. Savill J., Hogg N., Ren Y., Haslett C. Thrombospondin cooperates with CD36 and the vitronectin receptor in macrophage recognition of neutrophils undergoing apoptosis. J. Clin. Invest. 90(4):1992;1513.
136. Schmid-Schonbein G.W. Capillary plugging by granulocytes and the no-reflow phenomenon in the microcirculation. Fed. Proc. 46:1987;2397.
137. Schmid-Schonbein G.W., Simon S.I., Newcom D.R., Engler R.L. Effects of AQA-39 on granulocytes in the microcirculation of rat mesentery. Eur. Heart J. 8:1987;S75.
138. Sengelov H. Secretory vesicles of human neutrophils. Eur. J. Haematol. 57:1996;3.
139. Sengelov H., Kjeldsen L., Borregaard N. Control of exocytosis in early neutrophil activation. J. Immunol. 150:1993;1535.
140. Simon S.I., Chambers J.D., Sklar L.A. Flow cytometric analysis and modeling of cell-cell adhesive interactions: the neutrophil as a model. J. Cell Biol. 111:1990;2747.
141. 2+). Basic practical considerations. Methods Modern Biol. 114:1999;3.
142. Singer I.I., Scott S., Kawka D.W., Kazazis D.M. Adhesomes: specific granules containing receptors for laminin, C3bi/fibrinogen, fibronectin, and vitronectin in human polymorphonuclear leukocytes and monocytes. J. Cell Biol. 109:1989;3169.
143. Sjaastad, M.D., Nelson, W.J., 1997. Integrin-mediated calcium signaling and regulation of cell adhesion by intracellular calcium, 19, 47.
144. Sklar L.A., Oades Z.G. Signal transduction and ligand-receptor dynamics in the neutrophil. J. Biol. Chem. 260:1985;11468.
145. Sklar L.A., Hyslop P.A., Oades Z.G., Omann G.M., Jesaitis A.J., Painter R.G., Cochrane C.G. Signal transduction and ligand-receptor dynamics in the human neutrophil. Transient responses and occupancy-response relations at the formyl peptide receptor. J. Biol. Chem. 260:1985;11461.
146. Smedley L.A., Tonnesen M.G., Sandhaus R.A., Haslett C., Guthrie L.A., Johnston R.B. Jr., Henson P.M., Worthen G.S. Neutrophil-mediated injury to endothelial cells. Enhancement by endotoxin and essential role of neutrophil elastase. J. Clin. Invest. 77:1986;1233.
147. Smith J.A., Wiedemann M.J. Further characterization of the neutrophil oxidative burst by flow cytometry. J. Immunol. Methods. 162:1993;261.
148. Smyth S.S., Joneckis C.C., Parise L.V. Regulation of vascular integrins. Blood. 81:1993;2827.
149. Spitznagel J.K. Antibiotic proteins of human neutrophils. J. Clin. Invest. 86:1990;1381.
150. Springer T.A. Adhesion receptors of the immune system. Nature. 346:1990;425.
151. Springer T.A., Thompson W.S., Miller L.J., Schmalstieg F.C., Anderson D.C. Inherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis. J. Exp. Med. 160:1984;1901.
152. Steinkamp J.A., Wilson J.S., Saunders G.C., Stewart C.C. Phagocytosis: flow cytometric quantitation with fluorescent microspheres. Science. 215:1982;64.
153. Stewart C.C., Lehnert B.E., Steinkamp J.A. In vitro and in vivo measurement of phagocytosis by flow cytometry. Methods Enzymol. 132:1986;183.
154. Styrt B., Schwartz M.A., Klempner M.S. Calcium release associated with discharge of specific granule contents from human neutrophils. Biochem. Biophys. Res. Commun. 154:1988;1308.
155. Suzaki E., Kobayashi H., Kodama Y., Masujima T., Terakawa S. Video-rate dynamics of exocytotic events associated with phagocytosis in neutrophils. Cell Motil. Cytoskeleton. 38:1997;215.
156. Tabor B., Geissler B., Odell R., Schmidt B., Blumenstein M., Schindhelm K. Dialysis neutropenia: the role of the cytoskeleton. Kidney Int. 53:1998;783.
157. Takahashi A., Alnemri E.S., Lazebnik Y.A., Fernandes-Alnemri T., Litwack G., Moir R.D., Goldman R.D., Poirier G.G., Kaufmann S.H., Earnshaw W.C. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc. Natl. Acad. Sci. U.S.A. 93:1996;8395.
158. Tandon P., Diamond S.L. Kinetics of beta2-integrin and L-selectin bonding during neutrophil aggregation in shear flow. Biophys. J. 75:1998;3163.
159. Tanji-Matsuba K., van Eeden S.F., Saito Y., Okazawa M., Klut M.E., Hayashi S., Hogg J.C. Functional changes in aging polymorphonuclear leukocytes. Circulation. 97:1998;91.
160. Taylor A.D., Neelamegham S., Hellums J.D., Smith C.W., Simon S.I. Molecular dynamics of the transition from L-selectin- to beta 2-integrin-dependent neutrophil adhesion under defined hydrodynamic shear. Biophys. J. 71:1996;3488.
161. Treves S., de Virgilio F., Vaselli G.M., Pozzan T. Effect of cytochalasins on cytosolic-free calcium concentration and phosphoinositide metabolism in leukocytes. Exp. Cell Res. 168:1987;285.
162. van Eeden S.F., Miyagashima R., Haley L., Hogg J.C. L-selectin expression on peripheral blood polymorphonuclear leukocytes during active bone marrow release in humans. Am. J. Respir. Crit. Care Med. 151:1995;500.
163. van Eeden S.F., Bicknell S., Walker B.A., Hogg J.C. Polymorphonuclear leukocytes L-selectin expression decreases as they age in circulation. Am. J. Physiol. 272:1997;H401.
164. van Eeden S.F., Miyagashima R., Haley L., Hogg J.C. A possible role for L-selectin in the release of polymorphonuclear leukocytes from bone marrow. Am. J. Physiol. 272:1997;H1717.
165. van Eeden S.F., Zamfir D., Ferguson N., Hogg J.C. Polymorphonuclear leukocyte myeloperoxidase content in smokers. FASEB J. 11:1997;A127.
166. Vindenes, H.A., Bjerknes, R., 1997. Impaired actin polymerization and depolymerization in neutrophils from patients with thermal injury, 23, 131.
167. von Andrian U.H., Chambers J.D., McEvoy L.M., Bargatze R.F., Arfors K.E., Butcher E.C. Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. Proc. Natl. Acad. Sci. U.S.A. 88:1991;7538.
168. Vowells S.J., Sekhsaria S., Malech H.L., Shalit M., Fleischer T.A. Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J. Immunol. Methods. 178:1995;89.
169. Waddell T.K., Fialkow L., Chan C.K., Kishimoto T.K., Downey G.P. Potentiation of the oxidative burst of human neutrophils. A signaling role for L-selectin. J. Biol. Chem. 269:1994;18485.
170. Waisman D., van Eeden S.F., Hogg J.C., Solimano A., Massing B., Bondy G.P. L-selectin expression on polymorphonuclear leukocytes and monocytes in premature infants: reduced expression after dexamethasone treatment for bronchopulmonary dysplasia. J. Pediatr. 132(1):1998;53.
171. Walker B.A.M., Ward P.A. Priming and signal transduction in neutrophils. Biol. Signals. 1:1992;237.
172. Watts R.G., Howard T.H. Evidence for a gelsolin-rich, labile F-actin pool in human polymorphonuclear leukocytes. Cell Motil. Cytoskeleton. 21:1992;25.
173. Weiss S.J. Tissue destruction by neutrophils. N. Engl. J. Med. 320:1989;365.
174. White J.R., Naccache P.H., Molski T.F.P., Boregeat P., Shaafi R.L. Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor. Biochem. Biophys. Res. Commun. 113:1983;44.
175. White-Owen C., Alexander J.W., Sramkoski R.M., Babcock G.F. Rapid whole-blood microassay using flow cytometry for measuring neutrophil phagocytosis. J. Clin. Microbiol. 30:1992;2071.
176. Whyte M.K.B., Hardwick S.J., Meagher L.C., Savill J.S., Haslett C. Transient elevations of cytosolic free calcium retard subsequent apoptosis in neutrophils in vitro. J. Clin. Invest. 92:1993;446.
177. Whyte M.K., Meagher L.C., MacDermot J., Haslett C. Impairment of function in aging neutrophils is associated with apoptosis. J. Immunol. 150:1993;5124.
178. Wiggs B.R., English D., Quinlan W.M., Doyle N.A., Hogg J.C., Doerschuk C.M. Contributions of capillary pathway size and neutrophil deformability to neutrophil transit through rabbit lungs. J. Appl. Physiol. 77:1994;463.
179. Worthen G.S., Schwab B., Elson E.L. 3d., Downey G.P. Mechanics of stimulated neutrophils: cell stiffening induces retention in capillaries. Science. 245:1989;183.
180. Yee J., Christou N. Neutrophil priming by lipopolysaccharide involves heterogeneity in calcium-mediated signal transduction. J. Immunol. 150:1993;1989.
181. Youssef P.P., Mantzioris B.X., Roberts-Thomson P.J., Ahern M.J., Smith M.D. Effects of ex vivo manipulation on the expression of cell adhesion molecules on neutrophils. J. Immunol. Methods. 186:1995;217.
182. Zallen G., Moore E.E., Johnson J.L., Tamura D.Y., Aiboshi J., Biffl W.L., Silliman C.C. Circulating postinjury neutrophils are primed for the release of proinflammatory cytokines. J. Trauma. 46:1999;42.
183. Zamzami N., Marchetti P., Castedo M., Decaudin D., Macho A., Hirsch T., Susin S.A., Petit P.X., Mignotte B., Kroemer G. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. J. Exp. Med. 182:1995;367.