Phagocytosis: Measurement by flow cytometry

Journal of Immunological Methods

Volumn 243, Issue 1-2, 2000, Pages 229-242

  Lehmann, Anne K ^a   Sørnes, Steinar ^a   Halstensen, Alfred ^a  

^a UNIVERSITY OF BERGEN   (Norway)

Author keywords

Antigens; Flow cytometry (FCM); Opsonins; Oxidative burst; Phagocytosis

Indexed keywords

BACTERIAL ANTIGEN; FC RECEPTOR;

ANTIGEN SPECIFICITY; ARTICLE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEUKOCYTE; MEASUREMENT; NEISSERIA MENINGITIDIS; OPSONIZATION; PHAGOCYTOSIS; PRIORITY JOURNAL; RESPIRATORY BURST; STAPHYLOCOCCUS AUREUS;

FLOW CYTOMETRY; FLUORESCENT DYES; HUMAN; HYDROGEN-ION CONCENTRATION; LEUKOCYTES; OPSONINS; PHAGOCYTES; PHAGOCYTOSIS; RECEPTORS, COMPLEMENT; RECEPTORS, IGG; RESPIRATORY BURST;

EID: 0034699429     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00237-4     Document Type: Article

References (68)

1. Aase A., Bjune G., Høiby G., Rosenqvist E., Pedersen A.K., Michaelsen T. Comparison among opsonic activity, antimeningococcal immunoglobulin G response, and serum bactericidal activity against meningococci in sera from vaccinees after immunization with a serogroup B outer membrane vesicle vaccine. Infect. Immun. 63:1995;3531.
2. Antal P., Sipka S., Suranyi P., Csipo I., Seres T., Marodi L., Szegedi G. Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake. Ann. Hematol. 70:1995;259.
3. Bass D.A., Parce J.W., Dechatelet L.R., Szeida P., Seeds M.C., Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130:1983;1910.
4. Bassøe C.-F., Solsvik J., Laerum O.D. Quantitation of single cell phagocytic capacity by flow cytometry. Laerum O.D., Lindmo T., Thorud E. Flow Cytometry IV. 1980;170 Universitetsforlaget, Oslo, Norway.
5. Bassøe C.-F., Lærum O.D., Solberg C.O., Haneberg B. Phagocytosis of bacteria by human leukocytes measured by flow cytometry. Proc. Soc. Exp. Biol. Med. 174:1983;182.
6. Bassøe C.-F., Lærum O.-D., Glette J., Hopen G., Haneberg B., Solberg C.O. Simultaneous measurement of phagocytosis and phagosomal pH by flow cytometry. Role of polymorphonuclear neutrophilic leukocyte granules in phagosome acidification. Cytometry. 4:1983;254.
7. Bassøe C.F., Bjerknes R. The effect of serum opsonins on the phagocytosis of Staphylococcus aureus and zymosan particles, measured by flow cytometry. Acta Pathol. Microbiol. Immunol. Scand. (C). 92:1984;51.
8. Bjerknes R. Neutrophil phagocytosis: the influence of opsonins, neutrophil activation, and microbial characteristics. Robinson J.P., Babcock G.F. Phagocyte Function: A Guide for Research and Clinical Evaluation. 1998;187 Wiley-Liss, New York.
9. Bjerknes R., Bassøe C.-F. Human leukocyte phagocytosis of zymosan particles measured by flow cytometry. Acta Pathol. Microbiol. Immunol. Scand. (C). 91:1983;341.
10. Bjerknes R., Bassøe C.-F. Phagocyte C3-mediated attachment and internalization: flow cytometry studies using a fluorescence quenching technique. Blut. 12:1984;315.
11. Bjerknes R., Bassøe C.-F., Sjursen H., Laerum O.D., Solberg C.O. Flow cytometry for the study of phagocyte functions. Rev. Infect. Dis. 11:1989;16.
12. Bohmer R.H., Trinkle L.S., Staneck J.L. Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxicative burst. Cytometry. 13:1992;525.
13. Bøyum A. Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Invest. 21:(Suppl.):1968;97.
14. Bredius R.G., Derkx B.H., Fijen C.A., de Wit T.P., de Haas M., Weening R.S., van de Winkel J.G., Out T.A. Fc gamma receptor IIa (CD32) polymorphism in fulminant meningococcal septic shock in children. J. Infect. Dis. 170:1994;848.
15. Cantinieux B., Hariga C., Courtoy P., Hupin J., Fondu P. Staphylococcus aureus phagocytosis. A new cytofluorometric method using FITC and formaldehyde. J. Immunol. Methods. 121:1989;203.
16. Cinco M., Murgia R., Perticarari S., Presani G. Simultaneous measurement by flow cytometry of phagocytosis and metabolic burst induced in phagocytic cells in whole blood by Borrelia burgdorferi. FEMS Microbiol. Lett. 122:1994;187.
17. Densen P., Clark R.A., Nauseef W.M. Granulocytic phagocytes. Mandell G.L., Bennett J.E., Dolin R. Principles and Practice of Infectious Diseases. 1995;78 Churchill Livingstone, New York.
18. Dunn P.A., Tyrer H.W. Quantitation of neutrophil phagocytosis, using fluorescent latex beads. J. Lab. Clin. Med. 98:1981;374.
19. Duque R.E., Phan S.H., Hudson J.L., Till G.O., Ward P.A. Functional defects in phagocytic cells following thermal injury. Am. J. Pathol. 118:1985;116.
20. Faden H., Hong J.J., Pahade N. Immune response to Moraxella catarrhalis in children with otitis media: opsonophagocytosis with antigen-coated latex beads. Ann. Otol. Rhinol. Laryngol. 103:1994;522.
21. Fattorossi A., Nisini R., Pizzolo J.G., D'Amelio R. New, simple flow cytometry technique to discriminate between internalized and membrane-bound particles in phagocytosis. Cytometry. 10:1989;320.
22. Filatov M.V., Varfolomeeva E., Yu E., Ivanov E.I. Flow cytofluorometric detection of inflammatory processes by measuring respiratory burst reaction of peripheral blood neutrophils. Biochem. Mol. 55:1995;116.
23. Flø R.W., Naess A., Lund-Johansen F., Maehle B.O., Sjursen H., Lehmann V., Solberg C.O. Negative selection of human monocytes using magnetic particles covered by anti-lymphocyte antibodies. J. Immunol. Methods. 137:1991;89.
24. Fredriksen J.H., Rosenqvist E., Wedege E., Bryn K., Bjune G., Frøholm L.O., Lindbak A.K., Møgster B., Namork E., Rye U., Stabbetorp G., Winsnes R., Aase B., Closs O. Production, characterization and control of menB-vaccine 'Folkehelsa': an outer membrane vesicle vaccine against group B meningococcal disease. NIPH Ann. 14:1991;67.
25. Gallin J.I. Phagocytic cells: disorders of function. Gallin J.I., Goldstein I.M., Snyderman R. Inflammation: Basic Principles and Clinical Correlates. 1992;859 Raven Press, New York.
26. Guttormsen H.-K., Bjerknes R., Næss A., Lehmann V., Halstensen A., Sørnes S., Solberg C.O. Cross-reacting serum opsonins in patients with meningococcal disease. Infect. Immun. 60:1992;2777.
27. Guttormsen H.-K., Bjerknes R., Halstensen A., Næss A., Høiby E.A., Solberg C.O. Cross-reacting serum opsonins to meningococci after vaccination. J. Infect. Dis. 167:1993;1314.
28. Halstensen A., Haneberg B., Glette J., Sandberg S., Solberg C.O. Factors important for the measurements of chemiluminescence production by polymorphonuclear leukocytes. J. Immunol. Methods. 88:1986;121.
29. Halstensen A., Sjursen H., Vollset S.E., Frøholm L.O., Næss A., Matre R., Solberg C.O. Serum opsonins to serogroup B meningococci in meningococcal disease. Scand. J. Infect. Dis. 21:1989;267.
30. Hampton M.B., Winterbourn C.C. Methods for quantifying phagocytosis and bacterial killing by human neutrophils. J. Immunol. Methods. 232:1999;15.
31. Hasui M., Hirabayashi Y., Kobayashi Y. Simultaneous measurement by flow cytometry of phagocytosis and hydrogen peroxide production of neutrophils in whole blood. J. Immunol. Methods. 117:1989;53.
32. Hed J. Methods for distinguishing ingested from adhering particles. Methods Enzymol. 132:1986;198.
33. Klebanoff S.J., Clark F.A. The Neutrophil: Function and Clinical Disorders. 1978;Elsevier, Amsterdam.
34. Kuypers T.W., Eckmann C.M., Weening R.S., Roos D. A rapid turbidimetric assay of phagocytosis and serum opsonizing capacity. J. Immunol. Methods. 124:1989;85.
35. Leino L., Lilius E.M. The up- and down-modulation of immunoglobulin G Fc receptors and complement receptors on activated human neutrophils depends on the nature of activator. J. Leukocyte Biol. 51:1992;157.
36. Lehmann A.K., Halstensen A., Holst J., Bassøe C.-F. Functional assays for evaluation of serogroup B meningococcal structures as mediators of human opsonophagocytosis. J. Immunol. Methods. 200:1997;55.
37. Lehmann A.K., Halstensen A., Bassøe C.-F. Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens. Cytometry. 33:1998;6.
38. Lehmann A.K., Halstensen A., Aaberge I.S., Holst J., Michaelsen T.E., Sørnes S., Wetzler L.M., Guttormsen H.-K. Human opsonins induced during meningococcal disease recognize outer membrane proteins PorA and PorB. Infect. Immun. 67:1999;2552.
39. Lehmann A.K., Gorringe A.R., Reddin K., West K., Smith I., Halstensen A. Human opsonins induced during meningococcal disease recognize transferrin binding protein complexes. Infect. Immun. 67:1999;6526.
40. Lund-Johansen F., Olweus J., Horejsi V., Skubitz K.M., Thompson J.S., Vilella R., Symington F. Activation of human phagocytes through carbohydrate antigens (CD15, SIALYL-CD15, CDw17, and CDw65). J. Immunol. 148:1992;3221.
41. Martin E., Bhakdi S. Quantitative analysis of opsonophagocytosis and of killing of Candida albicans by human peripheral blood leukocytes by using flow cytometry. J. Clin. Microbiol. 29:1991;2013.
42. Martin E., Bhakdi S. Flow cytometric assay for quantifying opsonophagocytosis and killing of Staphylococcus aureus by peripheral blood leukocytes. J. Clin. Micrbiol. 30:1992;2246.
43. Ogle J.D., Noel J.G., Sramkoski R.M., Ogle C.K., Alexander J.W. Phagocytosis of opsonized fluorescent microspheres by human neutrophils. A two-color flow cytometric method for the determination of attachment and ingestion. J. Immunol. Methods. 115:1988;17.
44. Owen C.S., Sykes N.L. Magnetic labelling and cell sorting. J. Immunol. Methods. 73:1984;41.
45. Papadimitriou C.A., Roots A., Koenigsmann M., Koenigsmann M., Mucke C., Oelmann E., Oberberg D., Reufi B., Thiel E., Berdel W.E. Immunomagnetic selection of CD34+ cells from fresh peripheral blood mononuclear cell preparations using two different separation techniques. J. Hematother. 4:1995;539.
46. Partington K.M., Jenkinson E.J., Anderson G. A novel method of cell separation based on dual parameter immunomagnetic cell selection. J. Immunol. Methods. 223:1999;195.
47. Perticarari S., Presani G., Mangiarotti M.A., Banfi E. Simultaneous flow cytometric method to measure phagocytosis and oxicative products by neutrophils. Cytometry. 12:1991;687.
48. Perticarari S., Presani G., Banfi E. A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood. J. Immunol. Methods. 170:1994;117.
49. Peterson P.K., Verhoef J., Schmeling D., Quie P.G. Kinetics of phagocytosis and bacterial killing by human polymorphonuclear leukocytes and monocytes. J. Infect. Dis. 136:1977;502.
50. Richards G.A., Theron A.J., van der Merwe C.A., Anderson R. Spirometric abnormalities in young smokers correlate with increased chemiluminescence response of activated blood phagocytes. Am. Rev. Respir. Dis. 139:1989;181.
51. Richardson M.P., Ayliffe M.J., Helbert M., Davies E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J. Immunol. Methods. 219:1998;187.
52. Robinson J.P., Bruner L., Bassøe C.-F., Hudson J., Pon S., Ward P.A. Measurement of intracellular fluorescence of human monocytes relative to oxidative metabolism. J. Leukocyte Biol. 43:1988;304.
53. Roesler J., Hecht M., Freihorst J., Lohmann-Matthes M.L., Emmendorffer A. Diagnosis of chronic granulomatous disease and its mode of inheritance by dihydrorhodamine 123 and flow cytometry. Eur. J. Pediatr. 150:1990;161.
54. Rothe G., Oser A., Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenshaften. 75:1988;354.
55. Rothe G., Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′, 7′-dichlorofluorescein. J. Leukocyte Biol. 47:1990;440.
56. Rothe G., Emmendorffer A., Oser A., Roesler J., Valet G. Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123. J. Immunol. Methods. 138:1991;133.
57. Rothe G., Valet G. Flow cytometric assays of oxidative burst activity in phagocytes. Methods Enzymol. 233:1994;539.
58. Sanders L.A.M., Feldman R.G., Voorhorst-Ogink M.M., de Haas M., Rijkers G.T., Capel J.A., Zegers B.J.M., van de Winkel J.G.J. Human immunoglobulin G (Ig G) Fc receptor IIA (CD32) polymorphism and IgG2-mediated bacterial phagocytosis by neutrophils. Infect. Immun. 63:1995;73.
59. Santos J.L., Montes M.J., Gutierrez F., Ruiz C. Evaluation of phagocytic capacity with a modified flow cytometry technique. Immunol. Lett. 45:1995;1.
60. Saresella M., Roda K., Speciale L., Taramelli D., Mendozzi E., Guerini F., Ferrante P. A flow cytometric method for the analysis of phagocytosis and killing by polymorphonuclear leukocytes. Ann. NY Acad. Sci. 832:1997;53.
61. Sjursen H., Bjerknes R., Halstensen A., Næss A., Sørnes S., Solberg C.O. Flow cytometry assay for the measurement of serum opsonins to Neisseria meningitidis serogroup B, serotype 15. J. Immunol. Methods. 116:1989;235.
62. Steinkamp J.A., Stewart C.C., Crissman H.A. Three-color fluorescence measurements on single cell excited at three laser wavelengths. Cytometry. 2:1982;226.
63. Steinkamp J.A., Wilson J.S., Saunders G.C., Stewart C.C. Phagocytosis: flow cytometric quantitation with fluorescent microspheres. Science. 215:1982;64.
64. Szejda P., Parce J.W., Seeds M.S., Bass D.A. Flow cytometric quantitation of oxidative product formation by polymorphonuclear leukocytes during phagocytosis. J. Immunol. 133:1984;3303.
65. Van Eeden S.F., Klut M.E., Walker B.A., Hogg J.C. The use of flow cytometry to measure neutrophil function. J. Immunol. Methods. 232:1999;23.
66. Van Dilla M.A., Trujillo T.T., Mullaney P.F., Coulter J.R. Cell microfluorometry. A method for rapid fluorescence measurement. Science. 163:1969;1213.
67. Vowells S.J., Sekhsaria S., Malech H.L., Shalit M., Fleisher T.A. Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J. Immunol. Methods. 178:1995;89.
68. White-Owen C., Alexander J.W., Sramkoski R.M., Babcock G.F. Rapid whole-blood microassay using flow cytometry for measuring neutrophil phagocytosis. J. Clin. Microbiol. 30:1992;2071.