Kinesin's moonwalk

Current Opinion in Cell Biology

Volumn 18, Issue 1, 2006, Pages 61-67

  Carter, Nicholas J ^a   Cross, Robert A ^a  

^a MARIE CURIE RESEARCH INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; KINESIN; MOLECULAR MOTOR;

BINDING SITE; CONFORMATIONAL TRANSITION; FLUORESCENCE; MICROTUBULE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; REVIEW; STOICHIOMETRY;

ADENOSINE TRIPHOSPHATE; KINESIN; MICROTUBULES; MODELS, BIOLOGICAL; MOLECULAR MOTOR PROTEINS; MOVEMENT; PROTEIN CONFORMATION; TIME FACTORS;

EID: 30844467976     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ceb.2005.12.009     Document Type: Review

References (39)

1. Hackney DD: Motor proteins of the kinesin superfamily. In Energy Coupling and Molecular Motors. Edited by Hackney DD, Tamanoi F: Elsevier/Academic Press; 2003. The Enzymes, vol 23. A very useful survey of kinesin biophysics and biochemistry.
2. R.A. Cross The kinetic mechanism of kinesin Trends Biochem Sci 29 2004 301 309 A minireview that synthesises a great deal of kinetic data into a proposed general scheme involving three functional states of the active site.
3. K. Svoboda, and S.M. Block Force and velocity measured for single kinesin molecules Cell 77 1994 773 784
4. C.L. Asbury Kinesin: world's tiniest biped Curr Opin Cell Biol 17 2005 89 97 A thoughtful and balanced review of recently controversial aspects of the kinesin-1 stepping mechanism.
5. •] but also discusses the problem of how unconventional kinesins (those other than kinesin-1) move.
6. K. Kaseda, H. Higuchi, and K. Hirose Alternate fast and slow stepping of a heterodimeric kinesin molecule Nat Cell Biol 5 2003 1079 1082
7. C.L. Asbury, A.N. Fehr, and S.M. Block Kinesin moves by an asymmetric hand-over-hand mechanism Science 302 2003 2130 2134
8. H. Higuchi, C.E. Bronner, H.W. Park, and S.A. Endow Rapid double 8-nm steps by a kinesin mutant Embo J 23 2004 2993 2999 A homodimeric point mutant kinesin-1 dimer is shown to limp at high load but not at low load.
9. A. Yildiz, M. Tomishige, R.D. Vale, and P.R. Selvin Kinesin walks hand-over-hand Science 303 2004 676 678 A thought-provoking experiment in which the trajectory of a single fluorophore attached to one head of a walking kinesin molecule is tracked to nanometer precision. The data show a 16-nm periodicity, indicating that kinesin-1 spends most of its time with its heads 16 nm apart, or alternatively indicating that the labelled molecule is limping.
10. A.F. Huxley Muscle structure and theories of contraction Prog Biophys Biophys Chem 7 1957 257 318
11. A.F. Huxley, and R.M. Simmons Proposed mechanism of force generation in striated muscle Nature 233 1971 533 538
12. C. Veigel, F. Wang, M.L. Bartoo, J.R. Sellers, and J.E. Molloy The gated gait of the processive molecular motor, myosin V Nat Cell Biol 4 2002 59 65
13. C. Veigel, S. Schmitz, F. Wang, and J.R. Sellers Load-dependent kinetics of myosin-V can explain its high processivity Nat Cell Biol 7 2005 861 869 Development of the earlier gating experiments [12], using optical trapping to show that the heads of myosin V communicate by intermolecular strain.
14. M. Nishiyama, E. Muto, Y. Inoue, T. Yanagida, and H. Higuchi Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules Nat Cell Biol 3 2001 425 428
15. N.J. Carter, and R.A. Cross Mechanics of the kinesin step Nature 435 2005 308 312 This study demonstrates processive back-stepping by kinesin under backwards loads. The rate of back-stepping is dependent on ATP concentration, but is insensitive to the size of the backwards load. Step averaging is used to show that both forward and back steps are single events that occur on the microsecond time scale. The duration of these events is dependent on bead size, indicating that the measured step time is limited by bead diffusion.
16. Y. Okada, H. Higuchi, and N. Hirokawa Processivity of the single-headed kinesin KIF1A through biased binding to tubulin Nature 424 2003 574 577
17. T. Kamei, S. Kakuta, and H. Higuchi Biased binding of single molecules and continuous movement of multiple molecules of truncated single-headed kinesin Biophys J 88 2005 2068 2077
18. S.A. Endow, and H. Higuchi A mutant of the motor protein kinesin that moves in both directions on microtubules Nature 406 2000 913 916
19. M.J. Schnitzer, and S.M. Block Kinesin hydrolyses one ATP per 8-nm step Nature 388 1997 386 390
20. W. Hua, E.C. Young, M.L. Fleming, and J. Gelles Coupling of kinesin steps to ATP hydrolysis Nature 388 1997 390 393
21. D.L. Coy, M. Wagenbach, and J. Howard Kinesin takes one 8-nm step for each ATP that it hydrolyzes J Biol Chem 274 1999 3667 3671
22. K. Kawaguchi, and S. Ishiwata Nucleotide-dependent single- to double-headed binding of kinesin Science 291 2001 667 669
23. K. Kawaguchi, S. Uemura, and S. Ishiwata Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics Biophys J 84 2003 1103 1113
24. D.D. Hackney Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis Proc Natl Acad Sci USA 91 1994 6865 6869
25. K. Hirose, A. Lockhart, R.A. Cross, and L.A. Amos Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules Proc Natl Acad Sci USA 93 1996 9539 9544
26. I.M. Crevel, A. Lockhart, and R.A. Cross Weak and strong states of kinesin and ncd J Mol Biol 257 1996 66 76
27. S.S. Rosenfeld, P.M. Fordyce, G.M. Jefferson, P.H. King, and S.M. Block Stepping and stretching. How kinesin uses internal strain to walk processively J Biol Chem 278 2003 18550 18556
28. L.M. Klumpp, A. Hoenger, and S.P. Gilbert Kinesin's second step Proc Natl Acad Sci USA 101 2004 3444 3449
29. S.M. Block, C.L. Asbury, J.W. Shaevitz, and M.J. Lang Probing the kinesin reaction cycle with a 2D optical force clamp Proc Natl Acad Sci USA 100 2003 2351 2356
30. F. Gittes, E. Meyhofer, S. Baek, and J. Howard Directional loading of the kinesin motor molecule as it buckles a microtubule Biophys J 70 1996 418 429
31. ••].
32. M. Nishiyama, H. Higuchi, and T. Yanagida Chemomechanical coupling of the forward and backward steps of single kinesin molecules Nat Cell Biol 4 2002 790 797
33. H. Higuchi, E. Muto, Y. Inoue, and T. Yanagida Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP Proc Natl Acad Sci USA 94 1997 4395 4400
34. R.B. Case, S. Rice, C.L. Hart, B. Ly, and R.D. Vale Role of the kinesin neck linker and catalytic core in microtubule-based motility Curr Biol 10 2000 157 160
35. M. Tomishige, and R.D. Vale Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change J Cell Biol 151 2000 1081 1092
36. S. Rice, A.W. Lin, D. Safer, C.L. Hart, N. Naber, B.O. Carragher, S.M. Cain, E. Pechatnikova, E.M. Wilson-Kubalek, and M. Whittaker A structural change in the kinesin motor protein that drives motility Nature 402 1999 778 784
37. S. Rice, Y. Cui, C. Sindelar, N. Naber, M. Matuska, R. Vale, and R. Cooke Thermodynamic properties of the Kinesin neck-region docking to the catalytic core Biophys J 84 2003 1844 1854
38. D.D. Hackney, M.F. Stock, J. Moore, and R.A. Patterson Modulation of kinesin half-site ADP release and kinetic processivity by a spacer between the head groups Biochemistry 42 2003 12011 12018
39. M.J. Lang, P.M. Fordyce, A.M. Engh, K.C. Neuman, and S.M. Block Simultaneous, coincident optical trapping and single-molecule fluorescence Nat Methods 1 2004 133 139 An exciting preview of the next generation of single-molecule kinesin experiments.