The construction of the eukaryotic expression plasmid pcDNA3.1/azurin and the increased apoptosis of U2OS cells transfected with IT

Cellular and Molecular Biology Letters

Volumn 12, Issue 3, 2007, Pages 407-421

  Ye, Zhaoming ^a   Peng, Huiqin ^a   Fang, Yongming ^a   Feng, Jie ^a   Yang, Di Sheng ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Apoptosis; Azurin; Osteosarcoma; Transfection

Indexed keywords

AZURIN; COMPLEMENTARY DNA; EPITOPE; HEMAGGLUTININ; MESSENGER RNA; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53; SURVIVIN;

APOPTOSIS; ARTICLE; CELL STRAIN; CONTROLLED STUDY; DOWN REGULATION; EUKARYOTE; FLOW CYTOMETRY; GENE CONSTRUCT; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INFLUENZA VIRUS; NUCLEOTIDE SEQUENCE; OSTEOSARCOMA CELL; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; WESTERN BLOTTING;

EUKARYOTA; ORTHOMYXOVIRIDAE;

APOPTOSIS; AZURIN; BCL-2-ASSOCIATED X PROTEIN; BCL-X PROTEIN; BLOTTING, WESTERN; CELL CYCLE; CELL LINE, TUMOR; DNA FRAGMENTATION; EUKARYOTIC CELLS; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HUMANS; INHIBITOR OF APOPTOSIS PROTEINS; PLASMIDS; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 36749066515     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.2478/s11658-007-0012-3     Document Type: Article

References (25)

1. Sinha, G. Bacterial battalions join war against cancer. Nat. Med. 9 (2003) 1229.
2. Chakrabarty, A.M. Microorganisms and cancer: quest for a therapy. J. Bacteriol. 185 (2003) 2683-2686.
3. Kukimoto, M., Nishiyama, M., Tanokura, M., Murphy, M.E., Adman, E.T. and Horinouchi, S. Site-directed mutagenesis of azurin from Pseudomonas aeruginosa enhances the formation of an electron-transfer complex with a copper-containing nitrite reductase from Alcaligenes faecalis S-6. FEBS Lett. 394 (1996) 87-90.
4. Yamada, T., Fialho, A.M., Punj, V., Bratescu, L., Gupta, T.K. and Chakrabarty, A.M. Internalization of bacterial redox protein azurin in mammalian cells: entry domain and specificity. Cell. Microbiol. 7 (2005) 1418-1431.
5. Yamada, T., Hiraoka, Y., Ikehata, M., Kimbara, K., Avner, B.S., Das Gupta, T.K. and Chakrabarty, A.M. Apoptosis or growth arrest: Modulation of tumor suppressor p53's specificity by bacterial redox protein azurin. Proc. Natl. Acad. Sci. 101 (2004) 4770-4775.
6. Yang, D.S., Miao, X.D., Ye, Z.M., Feng, J., Xu, R.Z., Huang, X. and Ge, F.F. Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells. Pharmacol. Res. 52 (2005) 413-421.
7. Miao, X.D., Ye, Z.M., Yang, D.S., Xu, R.Z., Li, W.X. and Tao, H.M. Cytotoxicity and apoptosis of human osteosarcoma U2OS cells induced by recombinant soluble AZURIN. Zhejiang Da Xue Xue Bao Yi Xue Ban. 34 (2005) 384-389.
8. Wilson, I.A., Niman, H.L., Houghten, R.A., Cherenson, A.R., Connolly, M.L. and Lerner, R.A. The structure of an antigenic determinant in a protein. Cell 37 (1984) 767-778.
9. Feng, J., Zhao, L. and Yu, Q. Receptor-mediated stimulatory effect of oligochitosan in macrophages. Biochem. Biophys. Res. Commun. 317 (2004) 414-420.
10. Reed, J.C. Apoptosis-regulating proteins as targets for drug discovery. Trends. Mol. Med. 7 (2001) 314-319.
11. Wyllie, A.H. Apoptosis (the 1992 Frank Rose Memorial Lecture). Br. J. Cancer. 67 (1993) 205-208.
12. Zhou, M., Gu, L., Yeager, A.M. and Findley, H.W. Sensitivity to Fas-mediated apoptosis in pediatric acute lymphoblastic leukemia is associated with a mutant p53 phenotype and absence of Bcl-2 expression. Leukemia 12 (1998) 1756-1763.
13. Zhang, H., Heim, J. and Meyhack, B. Novel BNIP1 variants and their interaction with BCL2 family members. FEBS Lett. 448 (1999) 23-27.
14. Schlesinger, P.H., Gross, A., Yin, X.M., Yamamoto, K., Saito, M., Waksman, G. and Korsmeyer, S.J. Comparison of the ion channel characteristics of proapoptotic BAX and antiapoptotic BCL-2. Proc. Natl. Acad. Sci. 94 (1997) 11357-11362.
15. Tu, Y., Xu, F.H., Liu, J., Vescio, R., Berenson, J., Fady, C. and Lichtenstein, A. Upregulated expression of BCL-2 in multiple myeloma cells induced by exposure to doxorubicin, etoposide, and hydrogen peroxide. Blood 88 (1996) 1805-1812.
16. Salomons, G.S., Brady, H.J., Verwijs-Janssen, M., Van Den Berg, J.D., Hart, A.A., Van Den Berg, H., Behrendt, H., Hahlen, K. and Sme, L.A. The Bax alpha:Bcl-2 ratio modulates the response to dexamethasone in leukaemic cells and is highly variable in childhood acute leukaemia. Int. J. Cancer. 71 (1997) 959-965.
17. Li, F., Ambrosini, G., Chu, E.Y., Plescia, J., Tognin, S., Marchisio, P.C. and Altieri, D.C. Control of apoptosis and mitotic spindle checkpoint by Survivin. Nature 396 (1998) 580-584.
18. Yamada, T., Goto, M., Punj, V., Zaborina, O., Chen, M.L., Kimbara, K., Majumdar, D., Cunningham, E., Das Gupta, T.K. and Chakrabarty, A.M. Bacterial redox protein azurin, tumor suppressor protein p53, and regression of cancer. Proc. Natl. Acad. Sci. 99 (2002) 14098-14103.
19. Punj, V., Bhattacharyya, S., Saint-Dic, D., Vasu, C., Cunningham, E.A., Graves, J., Yamada, T., Constantinou, A.I., Christov, K., White, B., Li, G., Majumdar, D., Chakrabarty, A.M. and Das Gupta, TK. Bacterial cupredoxin azurin as an inducer of apoptosis and regression in human breast cancer. Oncogene 23 (2004) 2367-2378.
20. Yamada, T., Goto, M., Punj, V., Zaborina, O., Kimbara, K., Das Gupta, T.K. and Chakrabarty, A.M. The bacterial redox protein azurin induces apoptosis in J774 macrophages through complex formation and stabilization of the tumor suppressor protein p53. Infect. Immun. 70 (2002) 7054-7062.
21. Punj, V., Das Gupta, T.K. and Chakrabarty, A.M. Bacterial cupredoxin azurin and its interactions with the tumor suppressor protein p53. Biochem. Biophys. Res. Commun. 312 (2003) 109-114.
22. Vogelstein, B., Lane, D. and Levine, A.J. Surfing the p53 network. Nature 408 (2000) 307-310.
23. Haupt, Y., Maya, R., Kazaz, A. and Oren, M. Mdm2 promotes the rapid degradation of p53. Nature 387 (1997) 296-299.
24. Gross, A., Jockel, J., Wei, M.C. and Korsmeyer, S.J. Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis. EMBO J. 17 (1998) 3878-3885.
25. Budhram-Mahadeo, V., Morris, P.J., Smith, M.D., Midgley, C.A., Boxer, L.M. and Latchman, D.S. p53 suppresses the activation of the Bcl-2 promoter by the Brn-3a POU family transcription factor. J. Biol. Chem. 274 (1999) 15237-15244.