Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells

Protein Science

Volumn 16, Issue 12, 2007, Pages 2711-2715

  Welman, Arkadiusz ^a   Griffiths, John R ^b   Whetton, Anthony D ^b   Dive, Caroline ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Colon cancer; HCT116; Mass spectrometry; MIDAS; Phosphorylation; PKC ; Tet on

Indexed keywords

DOXYCYCLINE; PROTEIN KINASE C DELTA; SERINE; THREONINE;

ARTICLE; CANCER CELL CULTURE; CELL STRAIN HCT116; COLORECTAL CANCER; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PHOSPHORYLATION; GENE INDUCTION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; PRIORITY JOURNAL; TISSUE CULTURE;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; COLORECTAL NEOPLASMS; DOXYCYCLINE; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN KINASE C-DELTA; SERINE; THREONINE;

RATTUS;

EID: 36448992596     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.072874607     Document Type: Article

References (21)

1. Berens, C. and Hillen, W. 2003. Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. Eur. J. Biochem. 270: 3109-3121.
2. Brodie, C. and Blumberg, P.M. 2003. Regulation of cell apoptosis by protein kinase C delta. Apoptosis 8: 19-27.
3. Cerda, S.R., Mustafi, R., Little, H., Cohen, G., Khare, S., Moore, C., Majumder, P., and Bissonnette, M. 2006. Protein kinase C delta inhibits Caco-2 cell proliferation by selective changes in cell cycle and cell death regulators. Oncogene 25: 3123-3138.
4. Corbalan-Garcia, S. and Gomez-Fernandez, J.C. 2006. Protein kinase C regulatory domains: The art of decoding many different signals in membranes. Biochim. Biophys. Acta 1761: 633-654.
5. Gschwendt, M. 1999. Protein kinase C delta. Eur. J. Biochem. 259: 555-564.
6. Jackson, D.N. and Foster, D.A. 2004. The enigmatic protein kinase C delta: Complex roles in cell proliferation and survival. FASEB J. 18: 627-636.
7. Kikkawa, U., Matsuzaki, H., and Yamamoto, T. 2002. Protein kinase C delta (PKC delta): Activation mechanisms and functions. J. Biochem. 132: 831-839.
8. Koivunen, J., Aaltonen, V., and Peltonen, J. 2006. Protein kinase C (PKC) family in cancer progression. Cancer Lett. 235: 1-10.
9. 2-treated cells and its activation by tyrosine kinase in vitro. Proc. Natl. Acad. Sci. 98: 6587-6592.
10. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
11. Mellor, H. and Parker, P.J. 1998. The extended protein kinase C superfamily. Biochem. J. 332: 281-292.
12. Newton, A.C. 2001. Protein kinase C: Structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions. Chem. Rev. 101: 2353-2364.
13. Parekh, D.B., Ziegler, W., and Parker, P.J. 2000. Multiple pathways control protein kinase C phosphorylation. EMBO J. 19: 496-503.
14. Perletti, G., Marras, E., Dondi, D., Osti, D., Congiu, T., Ferrarese, R., de Eguileor, M., and Tashjian Jr., A.H. 2005. p21(Waf1/Cip1) and p53 are downstream effectors of protein kinase C delta in tumor suppression and differentiation in human colon cancer cells. Int. J. Cancer 113: 42-53.
15. Steinberg, S.F. 2004. Distinctive activation mechanisms and functions for protein kinase C delta. Biochem. J. 384: 449-459.
16. Teicher, B.A. 2006. Protein kinase C as a therapeutic target. Clin. Cancer Res. 12: 5336-5345.
17. Unwin, R.D., Craven, R.A., Harnden, P., Hanrahan, S., Totty, N., Knowles, M., Eardley, I., Selby, P.J., and Banks, R.E. 2003. Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect. Proteomics 3: 1620-1632.
18. Unwin, R.D., Griffiths, J.R., Leverentz, M.K., Grallert, A., Hagan, I.M., and Whetton, A.D. 2005. Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity. Mol. Cell. Proteomics 4: 1134-1144.
19. Welman, A., Cawthorne, C., Barraclough, J., Smith, N., Griffiths, G.J., Cowen, R.L., Williams, J.C., Stratford, I.J., and Dive, C. 2005. Construction and characterization of multiple human colon cancer cell lines for inducibly regulated gene expression. J. Cell. Biochem. 94: 1148-1162.
20. Welman, A., Barraclough, J., and Dive, C. 2006a. Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2. Nat. Protoc. 1: 803-811.
21. Welman, A., Cawthorne, C., Ponce-Perez, L., Barraclough, J., Danson, S., Murray, S., Cummings, J., Allen, T.D., and Dive, C. 2006b. Increases in CSrc expression level and activity do not promote the growth of human colorectal carcinoma cells in vitro and in vivo. Neoplasia 8: 905-916.