An approach to optimizing the active site in a glutathione transferase by evolution in vitro

Biochemical Journal

Volumn 344, Issue 1, 1999, Pages 93-100

  Hansson, Lars O ^a   Widersten, Mikael ^a   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Active site mutation; Activity screening; Glutathione transferase; Mutant library; Random mutagenesis

Indexed keywords

1 CHLORO 2,4 DINITROBENZENE; GLUTATHIONE; GLUTATHIONE TRANSFERASE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE ISOLATION; GENE MUTATION; PHAGE DISPLAY; POINT MUTATION; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; BASE SEQUENCE; CATALYTIC DOMAIN; DINITROCHLOROBENZENE; DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; GLUTATHIONE TRANSFERASE; HUMANS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY; VARIATION (GENETICS);

EID: 0033571041     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3440093     Document Type: Article

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