Optimized gene synthesis, expression and purification of active salivary plasminogen activator α2 (DSPAα2) of Desmodus rotundus in Pichia pastoris

Protein Expression and Purification

Volumn 57, Issue 1, 2008, Pages 27-33

  Wei, Zhaorong ^a   Wang, Yueju ^b   Li, Gangqiang ^a   Li, Xiaokun ^c   Liu, Dehu ^a,c  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

^c JILIN AGRICULTURAL UNIVERSITY   (China)

Author keywords

Gene synthesis; Optimized codon; Plasminogen activator 2; Vampire bat

Indexed keywords

FIBRIN; PLASMINOGEN ACTIVATOR; RECOMBINANT PROTEIN; SALIVA PROTEIN;

ANIMAL; ARTICLE; BAT; CHROMATOGRAPHY; CODON; CYTOMEGALOVIRUS; ENHANCER REGION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SECRETION; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CHIROPTERA; CHROMATOGRAPHY; CLONING, MOLECULAR; CODON; CYTOMEGALOVIRUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENHANCER ELEMENTS (GENETICS); ENZYME-LINKED IMMUNOSORBENT ASSAY; FIBRIN; GENETIC VECTORS; PICHIA; PLASMINOGEN ACTIVATORS; RECOMBINANT PROTEINS; SALIVARY PROTEINS;

DESMODUS ROTUNDUS; HUMAN HERPESVIRUS 5; PICHIA PASTORIS;

EID: 36248964608     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.09.001     Document Type: Article

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