High density fermentation and activity of a recombinant lumbrokinase (PI239) from Pichia pastoris

Protein Expression and Purification

Volumn 52, Issue 1, 2007, Pages 1-7

  Ge, Tao ^a   Fu, Shi Hong ^a   Xu, Li Hong ^a   Tang, Qing ^a   Wang, Huan Yu ^a   Guan, Kun Ping ^a   Liang, Guo Dong ^a  

^a Institute for Virus Disease Control and Provetion   (China)

Author keywords

Activity; Fermentation; Fibrinolytic; Lumbrokinase; Pichia pastoris

Indexed keywords

FUNGAL PROTEIN; LUMBROKINASE; PRIMER DNA; PROTEINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYMOLOGY; FERMENTATION; FIBRINOLYSIS; GENETIC ENGINEERING; GENETICS; KINETICS; METABOLISM; MOLECULAR WEIGHT; PICHIA; PLASMID; PROTHROMBIN TIME;

DNA PRIMERS; ENDOPEPTIDASES; FERMENTATION; FIBRINOLYSIS; FUNGAL PROTEINS; GENETIC ENGINEERING; KINETICS; MOLECULAR WEIGHT; PICHIA; PLASMIDS; PROTHROMBIN TIME; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 33845991877     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.027     Document Type: Article

References (27)

1. Tao G., and Liang G.D. The progress of lumbrokinase. J. Chinese Technol. 23 4 (2003) 48-52
2. Mihara H., Sumi H., Mizumoto H., Ikeda R., and Seiki M. A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rubellus. Jpn J. Physiol. 41 (1991) 461-472
3. Sumi N., Nakajima N., and Mihara H. Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci. Biotechnol. Biochem. 57 10 (1993) 1726-1730
4. Ryu G.H., Park S., Kim M., Han D.K., Kim Y.H., and Min B. J. Biomed. Mater. Res. 28 (1994) 1069-1077
5. Lu Y.H., Jin R.C., Wu Y.W., and Meng X.Q. Purification and characteristics of fibrinolytic enzyme from Amynthas dancataa. J. Biochem. 4 2 (1988) 166-172
6. Wu C., and Fan R. Acta Biophys. Sin. 2 (1986) 87
7. Shun Y.P., Ging Y.Y., Fan R., and Wu C. Capital Med. Sin. 5 (1998) 17
8. Tang Y., Liang D., Jiang T., Zhang J., Gui L., and Chang W. Crystal structure of earthworm fibrinolytic enzyme component A: revealing the structural determinants of its dual fibrinolytic activity. J. Mol. Biol. 321 (2002) 57-68
9. Sun Z.J., Liang G.D., Chen F., Fu S.H., Shen Y., and Hou Y.D. Cloning of cDNA of lumbrokinase and the effect on BHK cell. Chinese J. Biochem. Mol. Biol. 18 6 (2002) 776-779
10. Ge T., Sun Z.-J., FU S.-H., and Liang G.-D. Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in yeast P. pastoris. Protein Expr. Purif. 1 (2005) 2-3
11. Manabu S., and Nobuyoshi N. Molecular cloning, sequencing, and expression of cDNA encoding serine protease with fibrinolytic activity from earthworm. Biosci. Biotechnol. Biochem. 65 7 (2001) 1575-1580
12. Dong G.Q., Yuan X.L., Shan Y.J., Zhao Z.H., Chen J.P., and Cong Y.W. Molecular cloning and characterization of cDNA encoding fibrinolytic enzyme-3 from earthworm Eisenia foetida. Acta Biochim. Biophys. Sin. (Shanghai) 36 4 (2004) 303-308
13. Hu Y., Meng X.L., Xu J.P., Lu W., and Wang J. Cloning and expression of earthworm fibrinolytic enzyme PM(246) in Pichia pastoris. Protein Expr. Purif. 43 1 (2005) 18-25
14. Rongliang H., Shoufeng Z., Huiying L., Ning L., and Changchun T. Codon optimization, expression, and characterization of recombinant lumbrokinase in goat milk. Protein Expr. Purif. 37 (2004) 83-88
15. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (2003), Cold Spring Harbor Laboratory Press, New York
16. Invitrogen Corporation. A manual of methods for expression of recombinant proteins using pPICZ and pPICZα in Pichia pastoris. Catalog No.K1740-01, Version G, USA.
17. Heydorn W.E., Creed G.J., Goldman D., Kanter D., Merril C.R., and Jacobowitz D.M. Mapping and quantitation of proteins from discrete nuclei and other areas of the rat brain by two-dimensional gel electrophoresis. J. Neurosci. 3 12 (1983) 2597-2606
18. McGrew J.T., Leiske D., Dell B., Klinke R., Krasts D., Wee S.F., Abbott N., Armitage R., and Harrington K. Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants. Gene 187 (1997) 193-200
19. Holmquist M., Tessier D.C., and Cygler M. High-level production of recombinant Geotricum candidum lipases in yeast Pichia pastoris. Protein Expr. Purif. 11 (1997) 35-40
20. Invitrogen Corporation. Pichia Fermentation Process Guidelines. Version B, USA.
21. Shilin H. The anticoagulant activity and the toxicity of the earthworm's extract. J. Hum. Med. University 15 2 (1990) 107-110
22. Sasaki K., Hatsuoka M., Toki N., Sumi H., and Makita T. Fibrinolytic and coagulation activity level during formation of experimental thrombus in dog's saphena vein. Life Sci. 27 (1980) 1659-1665
23. Creeghino J.L., and Cregg J.M. Heterologous protein expression in methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 (2000) 45-66
24. Cregg J., Vedvick T., and Raschke W. Recent advances in the expression of foreign gene in Pichia pastoris. Bio/Technol. 11 (1993) 905-910
25. Bollini R., Ceriotti A., Daminati G.M., and Vitale A. Glycosylation is not needed for the intracellular transport of phytohemagglutinin in developing cotyledons and for the maintenance of its biological activities. Physiol. Planta 65 (1985) 15-22
26. Rayon C., Gomord V., Faye L., and Lerouge P. N-glycosylation of phytohemagglutinin expressed in bean cotyledons or in transgenic tobacco cells. Plant Physiol. Biochem. 34 (1996) 273-281
27. Sturm A., and Chrispeels M.J. The high mannose oligosaccharide of phytohemagglutinin is attached to asparagine-12 and the modified oligosaccharide to asparagine-60. Plant Physiol. 80 (1986) 320-322