Authentication of medicinal herbs using PCR-amplified ITS2 with specific primers

Planta Medica

Volumn 73, Issue 13, 2007, Pages 1421-1426

  Chiou, Shu Jiau ^a,b   Yen, Jui Hung ^b   Fang, Cheng Li ^b   Chen, Hui Ling ^b   Lin, Tsai Yun ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

Author keywords

Aristolochia fangchi; Aristolochia manshuriensis; Aristolochiaceae; Authentication; Clematis argentilucida; Internal transcribed spacer; Menispermaceae; Ranunculaceae; Stephania tetrandra

Indexed keywords

GENOMIC DNA; INTERNAL TRANSCRIBED SPACER; RIBOSOME DNA;

ARTICLE; DATA BASE; DNA EXTRACTION; GENE AMPLIFICATION; HERB; POLYMERASE CHAIN REACTION;

DNA PRIMERS; DNA, PLANT; DNA, RIBOSOMAL SPACER; DRUGS, CHINESE HERBAL; HUMANS; PHYTOTHERAPY; PLANTS, MEDICINAL; POLYMERASE CHAIN REACTION;

ARISTOLOCHIA FANGCHI; ARISTOLOCHIA MANSHURIENSIS; ARISTOLOCHIACEAE; CLEMATIS; MENISPERMACEAE; RANUNCULACEAE; STEPHANIA TETRANDRA;

EID: 36049006232     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2007-990227     Document Type: Article

References (17)

1. Shaw PC, Wang J, But PPH. Authentication of Chinese medicinal materials by DNA technology, 1st edition. Hackensack: World Scientific Publishing; 2002: 229-31
2. Lau DT, Shaw PC, Wang J, But PPH. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA. Planta Med 2001; 67: 456-60.
3. Zhao ZL, Zhou KY, Dong H, Xu LS. Characters of nrDNA ITS region sequences of fruits of Alpinia galanga and their adulterants. Planta Med 2001; 67: 381-3.
4. Shiba M, Kondo K, Miki E, Yamaji H, Morota T, Terabayashi S et al. Identification of medicinal Atractylodes based on ITS sequences of nrDNA. Biol Pharm Bull 2006; 29: 315-20.
5. Cubero OF, Crespo A, Fatehi J, Bridge PD. DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst Evol 1999; 216: 243-9.
6. Bailey CD, Carr TG, Harris SA, Hughes CE. Characterization of angiosperm nrDNA polymorphism, paralogy, and pseudogenes. Mol Phylogenet Evol 2003; 29: 435-55.
7. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Campbell CS, Donoghue MJ. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny. Ann Missouri Bot Gard 1995; 82: 247-77.
8. Ngan FN, Shaw PC, But PPH, Wang J. Molecular authentication of Panax species. Phytochem 1999; 50: 787-91.
9. Fu RZ, Wang J, Zhang YB, Wang ZT, But PPH, Li N et al. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism. Planta Med 1999; 67: 456-60.
10. Hillis DM, Dixon MT. Ribosomal DNA: Molecular evolution and phylogenetic interference. Q Rev Biol 1991; 66: 411-53.
11. LeRoy A, Potter E, Woo HH, Heber D, Hirsch AM. Characterization and identification of alfalfa and red clover dietary supplements using a PCR-based method. J Agric Food Chem 2002; 50: 5063-9.
12. Zhao Z, Hu Y, Liang Z, Yuen JP, Jiang Z, Leung KS. Authentication is fundamental for standardization of Chinese medicines. Planta Med 2006; 72: 865-74.
13. Alvarez I, Wendel JF. Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 2003; 29: 417-34.
14. Liu JS, Schardl CL. A conserved sequence in internal transcribed spacer 1 of plant nuclear rRNA genes. Plant Mol Biol 1994; 26: 775-8.
15. Jobes DV, Thien LB. A conserved motif in the 5.8S ribosomal RNA (rRNA) gene is a useful diagnostic marker for plant internal transcribed spacer (ITS) sequences. Plant Mol Biol Rep 1997; 15: 326-34.
16. Stiller JW, McClanahan A. Phyto-specific 16S rDNA PCR primers for recovering algal and plant sequences from mixed samples. Mol Ecol Notes 2005; 5: 1-3.
17. Kitano T, Umetsu K, Tian W, Osawa M. Two universal primer sets for species identification among vertebrates. Int J Legal Med online publication; 15 July 2006: doi: 10.1007/s00414-006-0113-y.