Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis

Electronic Journal of Biotechnology

Volumn 10, Issue 3, 2007, Pages 400-408

  Gothwal, Raj Kumar ^a   Nigam, Vinod Kumar ^a   Mohan, M Krishna ^a   Sasmal, Dinakar ^b   Ghosh, Purnendu ^a  

^a Birla Institute of Scientific Research   (India)

^b BIRLA INSTITUTE OF TECHNOLOGY   (India)

Author keywords

Arid soils; Microbial community; PCR DGGE; Rhizosphere; Soil DNA extraction

Indexed keywords

DNA; GENES; MICROORGANISMS; OPTIMIZATION; RNA; SOILS;

CALLIGONUM POLYGONOIDES; DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE); DESERT PLANT; DESERT SOILS; POLYMERASE CHAIN REACTION (PCR); SODIUM DODECYL SULPHATE (SDS);

BIOTECHNOLOGY;

DODECYL SULFATE SODIUM; GLASS; RNA 16S;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DESERT; DNA EXTRACTION; DNA TEMPLATE; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC CONSERVATION; LYSIS; NONHUMAN; PLANT; POLYMERASE CHAIN REACTION; RELIABILITY; RHIZOSPHERE; SOIL MICROFLORA;

BACTERIA (MICROORGANISMS); CALLIGONUM POLYGONOIDES;

EID: 36048953290     PISSN: None     EISSN: 07173458     Source Type: Journal    
DOI: 10.2225/vol10-issue3-fulltext-6     Document Type: Article

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