Soil community analysis using DGGE of 16S rDNA polymerase chain reaction products

Soil Science Society of America Journal

Volumn 64, Issue 4, 2000, Pages 1382-1388

  Nakatsu, Cindy H ^a   Torsvik, Vigdis ^b   Øvreås, Lise ^b  

^a PURDUE UNIVERSITY   (United States)

^b UNIVERSITY OF BERGEN   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

COMMUNITY STRUCTURE; DENATURING GRADIENT GEL ELECTROPHORESIS; GEL ELECTROPHORESIS; MICROBIAL COMMUNITY; NORWAY; POLLUTED SOIL; POLYCYCLIC AROMATIC HYDROCARBON; POLYMERASE CHAIN REACTION; RIBOSOMAL DNA; RIBOSOMAL RNA; UNITED STATES;

AROMATIC HYDROCARBONS; BACTERIA; CROPS; DNA; ELECTROPHORESIS; EXTRACTION;

DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE); POLYAROMATIC HYDROCARBONS (PAH); POLYMERASE CHAIN REACTIONS (PCR);

SOILS;

AGRICULTURAL SOIL; COMMUNITY COMPOSITION; DNA; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION;

NORWAY; UNITED STATES;

ARCHAEA; BACTERIA (MICROORGANISMS); CELLULAR ORGANISMS;

EID: 0034233490     PISSN: 03615995     EISSN: None     Source Type: Journal    
DOI: 10.2136/sssaj2000.6441382x     Document Type: Article

References (47)

1. The oligonucleotide probe database
2. Molecular approaches for environmental monitoring of microorganisms
3. Molecular microbial diversity of an agricultural soil in Wisconsin
4. Molecular microbial diversity in soils from eastern Amazonia - Evidence for unusual microorganisms and microbial population shifts associated with deforestation
5. Phylogenetic analysis of nonthermophilic members of the kingdom Crenarchaeota and their diversity and abundance in soils
6. Intraspecific variation in small-subunit rRNA sequences in Gen-Bank: Why single sequences may not adequately represent prokaryotic taxa
7. Phylogenetic stains: Ribosomal RNA-based probes for the identification of single cells
8. Phylogeny of the main bacterial 16S rRNA sequences in Drentse a grassland soils (the Netherlands)
9. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community
10. Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis
11. Widespread Archaea and novel Bacteria from the deep sea as shown by 16S rRNA gene sequences
12. Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific Oceans
13. Long-term tillage and rotation effects on corn growth and yield on high and low organic matter, poorly drained soils
14. Microbial evolution, diversity, and ecology - A decade of ribosomal RNA analysis of uncultivated microorganisms
15. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel electrophoretic separation in denaturing gradients
16. Size and activity of soil microbial communities in long-term experimental grassland plots treated with manure and inorganic fertilizers
17. Novel group within the kingdom Crenarchaeota from boreal forest soil
18. Bacterioplankton community diversity in a series of thermally stratified lakes
19. Microbial biomass and activity in lead-contaminated soil
20. Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments
21. 16S/23S rRNA Sequencing
22. Reverse transcriptase sequencing of ribosomal RNA for phylogenetic analysis
23. Estimating mass transfer of polyaromatic hydrocarbons from coal tar-contaminated soil
24. Bacterioplankton community composition in a boreal forest lake
25. A new version of the RDP (ribosomal database project)
26. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel
27. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
28. Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA-A new molecular approach to analyse the genetic diversity of mixed microbial communities
29. Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments
30. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis
31. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA
32. Microbial community changes in a perturbed agricultural soil investigated by molecular and physiological approaches
33. Microbial diversity and community structure in two different agricultural soil communities
34. Microbial communities, activity and biomass
35. An effective method to extract DNAs from environmental samples for PCR amplification and DNA fingerprint analysis
36. Differential amplification of rRNA genes by polymerase chain reaction
37. A method of establishing groups of equal amplitude in plant sociology based on similarity of species content
38. Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments
39. Novel techniques for analysing microbial diversity in natural and perturbed environments
40. High diversity in DNA of soil bacteria
41. Total bacterial diversity in soil and sediment communities - A review
42. Molecular phylogenetic analysis of a soil microbial community in a soybean field
43. A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil
44. Prokaryote systematics: The evolution of the science
45. Bacterial evolution
46. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids