The effect of small interfering RNA (siRNA) against the Bcl-2 gene on apoptosis and chemosensitivity in a canine mammary gland tumor cell line

Research in Veterinary Science

Volumn 84, Issue 1, 2008, Pages 49-55

  Nagamatsu, Kota ^a   Tsuchiya, Fumito ^a   Oguma, Keisuke ^a   Maruyama, Haruhiko ^a   Kano, Rui ^a   Hasegawa, Atsuhiko ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

Apoptosis; Bcl 2; Canine mammary gland tumor; Chemosensitivity; Doxorubicin; Small interfering RNA (siRNA)

Indexed keywords

DOXORUBICIN; LIPOSOME; PROTEIN BCL 2; SINGLE STRANDED DNA; SMALL INTERFERING RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; APOPTOSIS; ARTICLE; BREAST TUMOR; CELL VIABILITY; CHEMOSENSITIVITY; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; GENETIC TRANSFECTION; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE; WESTERN BLOTTING;

ANIMALS; ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL LINE, TUMOR; DOGS; DOSE-RESPONSE RELATIONSHIP, DRUG; DOWN-REGULATION; DOXORUBICIN; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, BCL-2; MAMMARY NEOPLASMS, ANIMAL; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 36048931108     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2007.03.012     Document Type: Article

References (48)

1. Adams J.M., and Cory S. The Bcl-2 protein family: arbiters of cell survival. Science 281 (1998) 1132-1322
2. Amundson S.A., Myers T.G., Scudiero D., Kitada S., Reed J.C., and Fornace Jr. A.J. An informatics approach identifying markers of chemosensitivity in human cancer cell lines. Cancer Research 60 (2000) 6101-6110
3. Beardsley A., Fang K., Mertz H., Castranova V., Friend S., and Liu J. Loss of caveolin-1 polarity impedes endothelial cell polarization and directional movement. The Journal of Biological Chemistry 280 (2005) 3541-3547
4. Bergman P.J. Mechanisms of anticancer drug resistance. The Veterinary Clinics of North America. Small Animal Practice 33 (2003) 651-667
5. Bettaieb A., Dubrez-Daloz L., Launay S., Plenchette S., Rebe C., Cathelin S., and Solary E. Bcl-2 proteins: targets and tools for chemosensitisation of tumor cells. Current Medicinal Chemistry. Anti-Cancer Agents 3 (2003) 307-318
6. Brodey R.S., Goldschmidt M.H., and Roszel J.R. Canine mammay, gland neoplasms. Journal of the American Animal Hospital Association 19 (1983) 61-90
7. Buchholz T.A., Garg A.K., Chakravarti N., Aggarwal B.B., Esteva F.J., Kuerer H.M., Singletary S.E., Hortobagyi G.N., Pusztai L., Cristofanilli M., and Sahin A.A. The nuclear transcription factor KB/bcl-2 pathway correlates with pathologic complete response to doxorubicin-based neoadjuvant chemotherapy in human breast cancer. Clinical Cancer Research 11 (2005) 8398-8402
8. Chi K.C., Wallis A.E., Lee C.H., De Menezes D.L., Sartor J., Dragowska W.H., and Mayer L.D. Effects of Bcl-2 modulation with G3139 antisense oligonucleotide on human breast cancer cells are independent of inherent Bcl-2 protein expression. Breast Cancer Research and Treatment 63 (2000) 199-212
9. Cleary M.L., Smith S.D., and Sklar J. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47 (1986) 19-28
10. Danilkovitch-Miagkova A., Miagkov A., Skeel A., Nakaigawa N., Zbar B., and Leonard E.J. Oncogenic mutants of RON and MET receptor tyrosine kinases cause activation of the β-catenin pathway. Molecular and Cellular Biology 21 (2001) 5857-5868
11. Davis J.M., Navolanic P.M., Weinstein-Oppenheimer C.R., Steelman L.S., Hu W., Konopleva M., Blagosklonny M.V., and McCubrey J.A. Raf-1 and Bcl-2 induce distinct and common pathways that contribute to breast cancer drug resistance. Clinical Cancer Research 9 (2003) 1161-1170
12. Decary S., Decesse J.T., Ogryzko V., Reed J.C., Naguibneva I., Harel-Bellan A., and Cremisi C.E. The retinoblastoma protein binds the promoter of the survival gene bcl-2 and regulates its transcription in epithelial cells through transcription factor AP-2. Molecular and Cellular Biology 22 (2002) 7877-7888
13. Dorn C.R., Taylor D.O., Schneider R., Hibbard H.H., and Klauber M.R. Survey of animal neoplasms in alameda and contra costa counties, California. II. Cancer morbidity in dogs and cats from Alameda County. Journal of the National Cancer Institute/National Institutes of Health 40 (1968) 307-318
14. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
15. Emi M., Kim R., Tanabe K., Uchida Y., and Toge T. Targeted therapy against Bcl-2-related proteins in breast cancer cells. Breast Cancer Research 7 (2005) 940-952
16. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., and Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
17. Frankfurt O.S., and Krishan A. Identification of apoptotic cells by formamide-induced DNA denaturation in condensed chromatin. The Journal of Histochemistry and Cytochemistry 49 (2001) 369-378
18. Frankfurt O.S., Robb J.A., Sugarbaker E.V., and Villa L. Monoclonal antibody to single-stranded DNA Is a specific and sensitive cellular marker of apoptosis. Experimental Cell Research 226 (1996) 387-397
19. Grone A., Weckmann M.T., Capen C.C., and Rosol T.J. Canine glyceraldehydes-3-phosphate dehydrogenase complementary DNA: polymerase chain reaction amplification, cloning, partial sequence analysis, and use as loading control in ribonuclease protection assays. American Journal of Veterinary Research 57 (1996) 254-257
20. Hershey A.E., Kurzman I.D., Forrest L.J., Bohling C.A., Stonerook M., Placke M.E., Imondi A.R., and Vail D.M. Inhalation chemotherapy for macroscopic primary or metastatic lung tumors: proof of principle using dogs with spontaneously occurring tumors as a model. Clinical Cancer Research 5 (1999) 2653-2659
21. Kumaraguruparan R., Karunagaran D., Balachandran C., Manohar B.M., and Nagini S. Of humans and canines: a comparative evaluation of heat shock and apoptosis-associated proteins in mammary tumors. Clinica Chimica Acta 365 (2006) 168-176
22. Lima R.T., Martins L.M., Guimaraes J.E., Sambade C., and Vasconcelos M.H. Specific downregulation of bcl-2 and xIAP by RNAi enhances the effects of chemotherapeutic agents in MCF-7 human breast cancer cells. Cancer Gene Therapy 11 (2004) 309-316
23. Macy D.W., Ensley B.A., and Gillette E.L. In vitro susceptibility of canine tumor stem cells to doxorubicin. American Journal of Veterinary Research 49 (1988) 1903-1905
24. Malich G., Markovic B., and Winder C. The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Toxicology 124 (1997) 179-192
25. Minakuchi Y., Takeshita F., Kosaka N., Sasaki H., Yamamoto Y., Kouno M., Honma K., Nagahara S., Hanai K., Sano A., Kato T., and Ochiya T. Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo. Nucleic Acids Research 32 (2004) e109
26. Minotti G., Menna P., Salvatorelli E., Cairo G., and Gianni L. Anthracyclines: Molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity. Pharmacology Reviews 56 (2004) 185-229
27. Miyashita T., and Reed J.C. Bcl-2 oncoprotein blocks chemotherapy-induced apoptosis in a human leukemia cell line. Blood 81 (1993) 151-157
28. Mizutani H., Tada-Oikawa S., Hiraku Y., Kojima M., and Kawanishi S. Mechanism of apoptosis induced by doxorubicin through the generation of hydrogen peroxide. Life Sciences 76 (2005) 1439-1453
29. Nogawa M., Yuasa T., Kimura S., Tanaka M., Kuroda J., Sato K., Yokota A., Segawa H., Toda Y., Kageyama S., Yoshiki T., Okada Y., and Maekawa T. Intravesical administration of small interfering RNA targeting PLK-1 successfully prevents the growth of bladder cancer. The Journal of Clinical Investigation 115 (2005) 978-985
30. Oltvai Z.N., Milliman C.L., and Korsmeyer S.J. Bcl-2 heterodimerizes in vivo with a conserved homolog, bax, that accelerates programmed cell death. Cell 74 (1993) 609-619
31. O'Toole S.A., Sheppard B.L., McGuinness E.P., Gleeson N.C., Yoneda M., and Bonnar J. The MTS assay as an indicator of chemosensitivity/resistance in malignant gynaecological tumours. Cancer Detection and Prevention 27 (2003) 47-54
32. Piche A., Grim J., Rancourt C., Gomez-Navarro J., Reed J.C., and Curiel D.T. Modulation of Bcl-2 protein levels by an intracellular anti-Bcl-2 single-chain antibody increases drug-induced cytotoxicity in the breast cancer cell line MCF-7. Cancer Research 58 (1998) 2134-2140
33. Reed J.C. Apoptosis-targeted therapies for cancer. Cancer Cell 3 (2003) 17-22
34. ® AQueous one solution cell proliferation assay. Promega Notes 59 (1996) 19
35. Ruiz de Almodovar C., Ruiz-Ruiz C., Munoz-Pinedo C., Robledo G., and Lopez-Rivas A. The differential sensitivity of Bc1-2-overexpressing human breast tumor cells to TRAIL or doxorubicin-induced apoptosis is dependent on Bc1-2 protein levels. Oncogene 20 (2001) 7128-7133
36. Simon D., Knebel J.W., Baumgartner W., Aufderheide M., Meyer-Lindenberg A., and Nolte I. In vitro efficacy of chemotherapeutics as determined by 50% inhibitory concentrations in cell cultures of mammary gland tumors obtained from dogs. American Journal of Veterinary Research 62 (2001) 1825-1830
37. Song E., Zhu P., Lee S.K., Chowdhury D., Kussman S., Dykxhoorn D.M., Feng Y., Palliser D., Weiner D.B., Shankar P., Marasco W.A., and Lieberman J. Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors. Nature Biotechnology 23 (2005) 709-717
38. Sorenmo K.U. Canine mammary gland tumors. The Veterinary Clinics of North America; Small Animal Practice; Update in Medical Oncology 33 (2003) 573-596
39. Sumimoto H., Yamagata S., Shimizu A., Miyoshi H., Mizuguchi H., Hayakawa T., Miyagishi M., Taira K., and Kawakami Y. Gene therapy for human small-cell lung carcinoma by inactivation of Skp-2 with virally mediated RNA interference. Gene Therapy 12 (2005) 95-100
40. Takahashi M., Saito H., Atsukawa K., Ebinuma H., Okuyama T., and Ishii H. Bcl-2 prevents doxorubicin-induced apoptosis of human liver cancer cells. Hepatology Research 25 (2003) 192-201
41. Takeshita F., and Ochiya T. Therapeutic potential of RNA interference against cancer. Cancer Science 97 (2006) 689-696
42. Takeshita, F., Minakuchi, Y., Nagahara, S., Honma, K., Sasaki, H, Hirai, K., Teratani, T., Namatame, N., Yamamoto, Y., Hanai, K., Kato, T., Sano, A. and Ochiya, T., 2005. Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo. In: Proceedings of the National Academy of Sciences of the United States of America. Biological sciences 23, pp. 12177-12182.
43. Teixeira C., Reed J.C., and Pratt M.A. Estrogen promotes chemotherapeutic drug resistance by a mechanism involving Bcl-2 proto-oncogene expression in human breast cancer cells. Cancer Research 55 (1995) 3902-3907
44. Tsujimoto, Y., Croce, C.M., 1986. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. In: Proceedings of the National Academy of Sciences of the United States of America. Biological sciences 83, pp. 5214-5218.
45. Whelan J. First clinical data on RNAi. Drug Discovery Today 10 (2005) 1014-1015
46. Yamashita A., Maruo K., Suzuki K., Shirota K., Kobayashi K., and Hioki K. Experimental chemotherapy against canine mammary cancer xenograft in SCID mice and its prediction of clinical effect. The Journal of Veterinary Medical Science 63 (2001) 831-836
47. Yano J., Hirabayashi K., Nakagawa S., Yamaguchi T., Nogawa M., Kashimori I., Naito H., Kitagawa H., Ishiyama K., Ohgi T., and Irimura T. Antitumor activity of small interfering RNA/cationic liposome complex in mouse models of cancer. Clinical Cancer Research 10 (2004) 7721-7726
48. Zhao J., Schmid-Kotsas A., Gross H.J., Gruenert A., and Bachem M.G. Sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells. Chinese Medical Journal 116 (2003) 1923-1929