Canine glyceraldehyde-3-phosphate dehydrogenase complementary DNA: Polymerase chain reaction amplification, cloning, partial sequence analysis, and use as loading control in ribonuclease protection assays

American Journal of Veterinary Research

Volumn 57, Issue 3, 1996, Pages 254-257

  Gröne, Andrea ^a   Weckmann, Michelle T ^a   Capen, Charles C ^a   Rosol, Thomas J ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; PRIMER DNA; RIBONUCLEASE;

ANIMAL; ARTICLE; BIOSYNTHESIS; DOG; GENETIC TRANSCRIPTION; GENETICS; HUMAN; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RAT; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; DOGS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; MICE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RATS; RIBONUCLEASES; RNA, MESSENGER; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSCRIPTION, GENETIC;

EID: 0030096230     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Glick AB, Lee MM, Darwiche N, et al Targeted deletion of the TGF-β 1 gene causes rapid progression to squamous cell carcinoma Genes Dev 1994,8.2429-2440.
2. Alwine JC, Kemp DJ, Stark GR. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes Proc Natl Acad Sci U S A 1977; 74:5350.
3. Gilman M. Ribonuclease protection assay In. Ausubel FM, ed. Current protocols, in molecular biology. Brooklyn, NY John Wiley & Sons Inc, 1994;4 7.1-4.7.8.
4. Hedges S, Agace W, Svensson M, et al Uroepithelial cells are part of a mucosal cytokine network Infect Immun 1994,62:2315-2321
5. Soifert NE, Van Why SK, Ganz MB, et al Expression of parathyroid hormone-related protein in the rat glomerulus and tubule during recovery from renal ischemia J Clin Invest 1993;92: 2850-2857.
6. Hillis DM, Dixon MT Ribosomal DNA. molecular evolution and phylogenetic inference. Q Rev Biol 1991,66:411-446
7. Onyia JE, Haalday DL, Messina JL. Identification of β-actin sequences necessary for induction by phorbol esters and calcium ionophores. Oncogene 1994;9:1713-1722.
8. Friedman J, Weissman I, Alpert S. An analysis of the expression of cyclophilin C reveals tissue restriction and an intriguing pattern in the mouse kidney Am J Pathol 1994,144:1247-1256
9. Ercolani L, Florence B, Denaro M, et al. Isolation and complete sequence of a functional glyceraldehyde-3-phosphate dehydrogenase gene. J Biol Chem 1988;263 15335-15341.
10. Tso JY, Koa XH, Reece KS, et al Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene Nucleic Acids Res 1985;13:2485-2502.
11. Merryman JI, Capen CC, McCauley LK, et al. Regulation of parathyroid hormone-related protein production by a squamous carcinoma cell line in vitro Lab Invest 1993,69:347-354
12. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
13. Yeh B, Svoboda KK. Intracellular distribution of β-actin is polarized in embryonic corneal epithelial cells J Cell Sci 1994,107. 105-115
14. Biragyn A, Arkins S, Kelley KW. Riboprobe expression cassettes for measuring IGF-I, β-actin and glyceraldehyde-3-phosphate dehydrogenase transcripts. J Immunol Methods 1994,168.235-244.
15. Schneider H, Charara N, Schmitz R, et al. Human cyclophilin C: primary structure, tissue distribution, and determination of binding specificity for cyclosporins. Biochemistry 1994;33:8218-8224
16. Basset-S'guin N, Demoly P, Moles JP, et al. Comparative analysis of cellular and tissular expression of c-fos in human keratinocytes, evidence of its role in cell differentiation. Oncogene 1994; 9:765-771
17. Löwik CWGM, Hoekman K, Offringa R, et al. Regulation of parathyroid hormonelike protein production in cultured normal and malignant keratinocytes. J Invest Dermatol 1992;98:198-203.
18. Bhatia P, Taylor WR, Greenberg AH, et al. Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28S-ribosomal RNA gene expression as RNA loading control for northern blot analysis of cell lines of various malignant potential. Anal Biochem 1994;216: 223-226.