Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.

Clinical Chemistry and Laboratory Medicine

Volumn 45, Issue 11, 2007, Pages 1464-1470

  Al Dahouk, Sascha ^a,b   Nöckler, Karsten ^c   Scholz, Holger C ^a   Pfeffer, Martin ^a   Neubauer, Heinrich ^a,d   Tomaso, Herbert ^a  

^a BUNDESWEHR INSTITUTE OF MICROBIOLOGY   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^d FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Brucella; Evaluation; Genus specific; Real time PCR; Species specific

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIOASSAY; BIOTYPE; BRUCELLA; CROSS REACTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENUS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SEROLOGY;

BASE SEQUENCE; BRUCELLA; DNA PRIMERS; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS; BRUCELLA MELITENSIS BIOVAR SUIS;

EID: 35649013907     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2007.305     Document Type: Article

References (23)

1. Al Dahouk S, Tomaso H, Nöckler K, Neubauer H, Frangoulidis D. Laboratory-based diagnosis of brucellosis - a review of the literature. Part I: techniques for direct detection and identification of Brucella spp. Clin Lab 2003;49:487-505.
2. Al Dahouk S, Tomaso H, Nöckler K, Neubauer H, Frangoulidis D. Laboratory-based diagnosis of brucellosis - a review of the literature. Part II: serological tests for brucellosis. Clin Lab 2003;49:577-89.
3. Verger JM, Grimont F, Grimont PA, Grayon M. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol 1985;35:292-5.
4. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994;11:2660-6.
5. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet Microbiol 2002;90:435-46.
6. García-Yoldi D, Marín CM, de Miguel MJ, Munoz PM, Vizmanos JL, López-Goni I. Multiplex PCR assay for the identification of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem 2006;52:779-81.
7. Redkar R, Rose S, Bricker B, DelVecchio V. Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis. Mol Cell Probes 2001;15:43-52.
8. Newby DT, Hadfield TL, Roberto FF. Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5′-exonuclease, and hybridization probe assays. Appl Environ Microbiol 2003;69:4753-9.
9. Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol 2004;42:1290-3.
10. Bogdanovich T, Skurnik M, Lubeck PS, Ahrens P, Hoorfar J. Validated 5′ nuclease PCR assay for rapid identification of the genus Brucella. J Clin Microbiol 2004;42:2261-3.
11. Navarro E, Segura JC, Castano MJ, Solera J. Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin Infect Dis 2006;42:1266-73.
12. Debeaumont C, Falconnet PA, Maurin M. Real-time PCR for detection of Brucella spp. DNA in human serum samples. Eur J Clin Microbiol Infect Dis 2005;24:842-5.
13. Colmenero JD, Queipo-Ortuno MI, Reguera JM, Baeza G, Salazar JA, Morata P. Real time polymerase chain reaction: a new powerful tool for the diagnosis of neurobrucellosis. J Neurol Neurosurg Psychiatry 2005;76:1025-7.
14. Queipo-Ortuno MI, Colmenero JD, Baeza G, Morata P. Comparison between LightCycler real-time polymerase chain reaction (PCR) assay with serum and PCR-enzyme-linked immunosorbent assay with whole blood samples for the diagnosis of human brucellosis. Clin Infect Dis 2005;40:260-4.
15. Queipo-Ortuno MI, Colmenero JD, Munoz N, Baeza G, Clavijo E, Morata P. Rapid diagnosis of Brucella epididymo-orchitis by real-time polymerase chain reaction assay in urine samples. J Urol 2006;176:2290-3.
16. Queipo-Ortuno MI, Colmenero JD, Reguera JM, Garcia-Ordonez MA, Pachon ME, Gonzalez M, et al. Rapid diagnosis of human brucellosis by SYBR green I-based real-time PCR assay and melting curve analysis in serum samples. Clin Microbiol Infect 2005;11:713-8.
17. Apfalter P, Reischl U, Hammerschlag MR. In-house nucleic acid amplification assays in research: how much quality control is needed before one can rely upon the results? J Clin Microbiol 2005;43:5835-41.
18. Alton GG, Jones LM, Angus RD, Verger JM, editors. Techniques for the brucellosis laboratory. Paris: Institut National de la Recherche Agronomique, 1988.
19. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 1992;95:271-5.
20. Tomaso H, Reisinger EC, Al Dahouk S, Frangoulidis D, Rakin A, Landt O, et al. Rapid detection of Yersinia pestis with multiplex real-time PCR assays using fluorescent hybridisation probes. FEMS Immunol Med Microbiol 2003;38:117-26.
21. Christensen DR, Hartman LJ, Loveless BM, Frye MS, Shipley MA, Bridge DL, et al. Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. Clin Chem 2006;52:141-5.
22. Morata P, Queipo-Ortuno MI, Reguera JM, Miralles F, Lopez-Gonzales JJ, Colmenero JD. Diagnostic yield of a PCR assay in focal complications of brucellosis. J Clin Microbiol 2001;39:3743-6.
23. Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ. Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J Clin Microbiol 2001;39:1661-4.