Laboratory-based diagnosis of brucellosis - A review of the literature

Clinical Laboratory

Volumn 49, Issue 11-12, 2003, Pages 577-589

  Al Dahouk, Sascha ^a   Tomaso, Herbert ^a   Nöckler, Karsten ^b   Neubauer, Heinrich ^a   Frangoulidis, Dimitrios ^a  

^a Institute of Microbiology   (Germany)

^b FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

Brucella; Brucella ELISA; Brucellosis; Serological tests

Indexed keywords

ANTIBIOTIC AGENT; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; ROSE BENGAL;

AGGLUTINATION TEST; ANTIBODY SPECIFICITY; ANTIBODY TITER; ARTHRALGIA; ARTHROPATHY; BACTERIUM CULTURE; BACTERIUM ISOLATION; BLOOD CULTURE; BONE DISEASE; BONE MARROW CULTURE; BRAIN DISEASE; BRUCELLA ABORTUS; BRUCELLA CANIS; BRUCELLA MELITENSIS; BRUCELLA SUIS; BRUCELLOSIS; CHILL; CHRONIC DISEASE; COMPLEMENT FIXATION TEST; COOMBS TEST; DISEASE SEVERITY; ENDOCARDITIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FATALITY; FATIGUE; FEVER; FLUORESCENCE POLARIZATION; HAZARD ASSESSMENT; HEADACHE; HUMAN; IMMUNOFLUORESCENCE TEST; LABORATORY DIAGNOSIS; MALAISE; MUSCLE WEAKNESS; PHENOTYPE; PHYSICIAN; RADIOIMMUNOASSAY; RELAPSE; REVIEW; SENSITIVITY AND SPECIFICITY; SEROLOGY; SPECIES DIFFERENCE; SYMPTOMATOLOGY; TISSUE CULTURE; TREATMENT FAILURE;

AGGLUTINATION TESTS; ANTIBODIES, BACTERIAL; BRUCELLA; BRUCELLOSIS; COOMBS' TEST; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BACTERIA (MICROORGANISMS); BRUCELLA; BRUCELLA MELITENSIS; BRUCELLA MELITENSIS BIOVAR ABORTUS; BRUCELLA MELITENSIS BIOVAR CANIS; BRUCELLA MELITENSIS BIOVAR SUIS; CANIS; NEGIBACTERIA;

EID: 0345601796     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (89)

1. Al-Harthi SS. The morbidity and mortality pattern of Brucella endocarditis. Int J Cardiol 1989; 25: 321-4
2. Al-Shamahy HA, Wright SG. Enzyme-linked immunosorbent assay for Brucella antigen detection in human sera. J Med Microbiol 1998; 47: 169-72
3. Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Paris: INRA, 1988
4. Alton GG, Maw J, Rogerson BA, McPherson GG. The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and Rose Bengal tests. Aust Vet J 1975; 51: 57-63
5. Anderson RK, Jenness R, Brumfield HP, Gough P. Brucella-agglutinating antibodies: relation of mercaptoethanol stability to complement fixation. Science 1964; 143: 1334-5
6. Araj GF, Awar GN. The value of ELISA vs. negative Coombs' findings in the serodiagnosis of human brucellosis. Serodiag Immunother Infect Dis 1997; 8: 169-72
7. Araj GF, Dhar R, Lastimoza JL, Haj M. Indirect fluorescent antibody test vs. ELISA and agglutination tests in the serodiagnosis of patients with brucellosis. Serodiag Immunother Infect Dis 1990; 4: 1-8
8. Araj GF, Kaufmann AF. Determination by enzyme-linked immunosorbent assay of immunoglobulin G (IgG), IgM, and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis. J Clin Microbiol 1989; 27: 1909-12
9. Araj GF. Human brucellosis: a classical infectious disease with persistent diagnostic challenges. Clin Lab Sci 1999; 12: 207-12
10. Ariza J, Pellicer T, Pallarés R, Foz A, Gudiol F. Specific antibody profile in human brucellosis. Clin Infect Disease 1992; 14: 131-40
11. Baldi PC, Miguel SE, Fossati CA, Wallach JC. Serological follow-up of human brucellosis by measuring IgG antibodies to lipopolysaccharide and cytoplasmic proteins of Brucella species. Clin Infect Diseases 1996; 22: 446-55
12. Baum M, Zamir O, Bergman-Rios R et al. Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. J Clin Microbiol 1995; 33: 2166-70
13. Bettelheim KA, Maskill WJ, Pearce J. Comparison of standard tube and microagglutination techniques for determining Brucella antibodies. J Hyg (Lond) 1983; 90: 33-9
14. Brown SL, Klein GC, McKinney FT, Jones WL. Safranin-O-stained antigen microagglutination test for detection of Brucella antibodies. J Clin Microbiol 1981; 13: 398-400
15. Buchanan TM, Faber LC. 2-Mercaptoethanol Brucella agglutination test: usefulness for predicting recovery from brucellosis. J Clin Microbiol 1980; 11: 691-3
16. Buchanan TM, Sulzer CR, Frix MK, Feldman RA. Brucellosis in the United States, 1960-1972: an abattoir-associated disease. II. Diagnostic aspects. Medicine 1974; 53: 415-26
17. Caravano R, Chabaud F, Oberti J. Application of immunoenzymatic techniques for epidemiological surveys on brucellosis among human populations. Ann Inst Pasteur Microbiol 1987; 138: 79-84
18. Chand P, Sadana JR, Batra HV, Chauhan RS. Comparison of the dot-immunobinding assay with the complement fixation test for the detection of Brucella antibodies in sheep. Vet Microbiol 1989; 20: 281-7
19. Chand P, Sadana JR, Batra HV, Khanna RNS. Comparison of dot enzyme-linked immunosorbent assay (dot-ELISA) with other serological tests for the detection of Brucella antibodies in bovine sera. J Vet Med 1989; 36: 346-52
20. Coghlan JD, Weir DM. Antibodies in human brucellosis. Br Med J 1967; 2: 269-71
21. Corbel MJ, Stuart FA, Brewer RA. Observations on serological cross-reactions between smooth Brucella species and organisms of other genera. Dev Biol Stand 1984; 56: 341-8
22. Dabdoob WA, Abdulla ZA. A panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis. Eastern Mediterranean Health Journal 2000; 6: 304-12
23. De Klerk E, Anderson R. Comparative evaluation of the enzyme-linked immunosorbent assay in the laboratory diagnosis of brucellosis. J Clin Microbiol 1985; 21: 381-6
24. Deutsch HF, Morton JI. Dissociation of human serum macroglobulins. Science 1957; 125: 600-1
25. Díaz R, Moriyón I. Laboratory techniques in the diagnosis of human brucellosis. In: Young EJ, Corbel MJ (eds) Brucellosis: clinical and laboratory aspects. Florida: CRC Press, 1989: 73-83
26. Edwards JM, Tannahill AJ, Bradstreet CM. Comparison of the indirect fluorescent antibody test with agglutination, complement-fixation, and Coombs' test for Brucella antibody. J Clin Pathol 1970; 23: 161-5
27. Fernandez-Lago L, Díaz R. Demonstration of antibodies against Brucella melitensis 16 M lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay. J Clin Microbiol 1986; 24: 76-80
28. Foz A, Pellicer T, Comerma J, Ariza J. Specificity of ELISA anti-immunoglobulin G conjugate in the diagnosis of human brucellosis. Eur J Clin Microbiol 1985; 4: 138-9
29. Gad El-Rab MO, Kambal AM. Evaluation of Brucella enzyme immunoassay test (ELISA) in comparison with bacteriological culture and agglutination. J Infect 1998; 36: 197-201
30. Gall D, Nielsen K, Forbes L et al. Validation of the fluorescence polarization assay and comparison to other serological assays for the detection of serum antibodies to Brucella abortus in bison. J Wildlife Dis 2000; 36: 469-76
31. Gazapo E, Lahoz JG, Subiza JL, Baquero M, Gil J, de la Concha EG. Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up. J Infect Diseases 1989; 159: 219-25
32. Goldbaum FA, Rubbi CP, Wallach JC, Miguel SE, Baldi PC, Fossati CA. Differentiation between active and inactive human brucellosis by measuring antiprotein humoral immune responses. J Clin Microbiol 1992; 30: 604-7
33. Gürtürk K, Boynukara B, Ilhan Z, Hakki Ekin I, Gülhan T. Comparison of the dot-immunobinding assay with the serum agglutination test, the Rose Bengal plate test and the milk ring test for the detection of Brucella antibodies in bovine sera and milk. J Vet Med 1999; 46: 279-85
34. Hall WH, Manion RE. Comparison of the Coombs' test with other methods for Brucella agglutinins in human serum. J Clin Invest 1953; 32: 96-106
35. Henderson RJ, Hill DM, Vickers AA, Edwards JM, Tillett H. Correlation between serological and immunofluorescence results in the investigation of brucellosis in veterinary surgeons. J Clin Pathol 1976; 29: 35-8
36. 1A-globulin fractions. J Immunol 1963; 91: 11-7
37. Hewitt WG, Payne DJH. Estimation of IgG and IgM Brucella antibodies in infected and non-infected persons by a radioimmune technique. J Clin Pathol 1984; 37: 692-6
38. Ismail TF, Smits H, Wasfy MO, Malone JL, Fadeel MA, Mahoney F. Evaluation of dipstick serologic tests for diagnosis of brucellosis and typhoid fever in Egypt. J Clin Microbiol 2002; 40: 3509-11
39. Jones LM, Hendricks JB, Berman DT. The standardization and use of the complement fixation test for the diagnosis of bovine brucellosis, with a review of the literature. Am J Vet Res 1963; 24: 1143-51
40. Jones LO, Wilson MM. Serum agglutinins in brucellosis. Nature 1951; 167: 558-9
41. Jumba MM, Mirza NB, Mwaura FB. Agglutinins for Brucellae antigens in blood sera of an urban and rural population in Kenya. East African Medical Journal 1996; 73: 204-6
42. Kerr WR, Coghlan JD, Payne DJH, Robertson L. The laboratory diagnosis of chronic brucellosis. Lancet 1966; 11: 1181-3
43. Kerr WR, McCaughey WJ, Coghlan JD et al. Techniques and interpretations in the serological diagnosis of brucellosis in man. J Med Microbiol 1968; 1: 181-93
44. Kerr WR, Payne DJH, Robertson L, Coombs RRA. Immunoglobulin class of Brucella antibodies in human sera. Immunology 1967; 13: 223-5
45. Khan MS, Humayoon MS, Al Manee MS. Epididymo-orchitis and brucellosis. Br J Urol 1989; 63: 87-9
46. Klein GC, Behan KA. Determination of Brucella immunoglobulin G agglutinating antibody titer with dithiothreitol. J Clin Microbiol 1981; 14: 24-5
47. Lindberg AA, Haeggman S, Karlson K, Carlsson HE, Mair NS. Enzyme immumoassay of the antibody response to Brucella and Yersinia enterocolitica O:9 infections in human. J Hyg (Lond) 1982; 88: 295-307
48. Lubani MM, Lulu AR, Araj GF, Khateeb MI, Quortom MAF, Dudin KI. Pulmonary brucellosis. Q J Med 1989; 71: 319-24
49. Lucero NE, Bolpe JE. Buffered plate antigen test as a screening test for diagnosis of human brucellosis. J Clin Microbiol 1998; 36: 1425-7
50. Lucero NE, Foglia L, Ayala SM, Gall D, Nielsen K. Competitive enzyme immunoassay for diagnosis of human brucellosis. J Clin Microbiol 1999; 37: 3245-8
51. Lulu AR, Araj GF, Khateeb ML. Human brucellosis in Kuwait: a prospective study of 400 cases. Q J Med 1988; 66: 39-54
52. MacDonald A, Elmslie WH. Serological investigations in suspected brucellosis. Lancet 1967; 1: 380-2
53. MacMillan AP, Cockrem DS. Reduction of nonspecific reactions to the Brucella abortus serum agglutination test by the addition of EDTA. Res Vet Sci 1985; 38: 288-91
54. Magee JT. An enzyme-labeled immunosorbent assay for Brucella abortus antibodies. J Med Microbiol 1980; 13: 167-72
55. Marrodan T, Nenova-Poliakova R, Rubio M et al. Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests. J Med Microbiol 2001; 50: 663-6
56. Memish ZA, Almuneef M, Mah MW, Qassem LA, Osoba AO. Comparison of the Brucella standard agglutination test with the ELISA IgG and IgM in patients with Brucella bacteremia. Diagn Microbiol Infect Dis 2002; 44: 129-32
57. Morgan WJB, MacKinnon DJ, Cullen GA. The Rose Bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec 1969; 85: 636-41
58. Mousa ARM, Muhtaseb SA, Almudallal DS, Khodeir SM, Marafie AA. Osteoarticular complications of brucellosis: a study of 169 cases. Rev Infect Dis 1987; 9: 531-43
59. Moyer NP, Evins GM, Pigott NE et al. Comparison of serologic screening tests for brucellosis. J Clin Microbiol 1987; 25: 1669-72
60. Nicoletti P, Muraschi TF. Bacteriologic evaluation of serologic test procedures for the diagnosis of brucellosis in problem cattle herds. Am J Vet Res 1966; 27: 689-94
61. Nielsen K, Gall D, Jolley M et al. A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus. J Immunol Methods 1996; 195: 161-8
62. Nielsen K, Gall D, Smith P et al. Fluorescence polarization assay for the diagnosis of bovine brucellosis: adaptation to field use. Vet Microbiol 2001; 80: 163-70
63. Nielsen K, Kelly L, Gall D et al. The use of divalent cation chelating agents (EDTA/EGTA) to reduce nonspecific protein interaction in enzyme immunoassay. Vet Res Communications 1994; 18: 433-7
64. Nielsen K, Kelly L, Gall D, Balsevicius S, Nicoletti P, Kelly W. Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis. Vet Immunol Immunopathol 1995; 46: 285-91
65. Nielsen KH, Kelly L, Gall D et al. Comparison of enzyme immunoassays for the diagnosis of bovine brucellosis. Prev Vet Med 1996; 26: 17-32
66. Orduna A, Almaraz A, Prado A et al. Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis. J Clin Microbiol 2000; 38: 4000-5
67. Osoba AO, Balkhy H, Memish Z et al. Diagnostic value of Brucella ELISA IgG and IgM in bacteremic and non-bacteremic patients with brucellosis. J Chemotherapy 2001;13:54-9
68. Otero JR, Fuertes A, Palenque E, Noriega AR. Microtiter-adapted method that facilitates the Coombs' test for brucellosis. J Clin Microbiol 1982; 16: 737-8
69. Parrat D, Nielsen KH, White RG, Payne DJH. Radioimmunoassay of IgM, IgG, and IgA Brucella antibodies. Lancet 1977; i: 1075-8
70. Patterson JM, Deyoe BL, Stone SS. Identification of immunoglobulins associated with complement fixation, agglutination, and low pH buffered antigen tests for brucellosis. Am J Vet Res 1976; 37: 319-24
71. Pellicer T, Ariza J, Foz A, Pallarés R, Gudiol F. Specific antibodies detected during relapse of human brucellosis. J Infect Disease 1988; 157: 918-24
72. Pike RM. Past and present hazards of working with infectious agents. Arch Pathol Lab Med 1978; 102: 333-6
73. Reddin JL, Anderson RK, Jenness R, Spink WW. Significance of 7S and macroglobulin Brucella agglutinins in human brucellosis. N Engl J Med 1965; 24: 1263-8
74. Rose JE, Roepke MH. An acidified antigen for detection of nonspecific reactions in the plate-agglutination test for bovine brucellosis. Am J Vet Res 1957; 18: 550-5
75. Russell AO, Patton CM, Kaufmann AF. Evaluation of the card test for diagnosis of human brucellosis. J Clin Microbiol 1978; 7: 454-8
76. Samartino L, Gregoret R, Gall D, Nielsen K. Fluorescence polarization assay: application to the diagnosis of bovine brucellosis in Argentina. J Immunoassay 1999; 20: 115-26
77. Sippel JE, El-Masry NA, Farid Z. Diagnosis of human brucellosis with ELISA. Lancet 1982; 2: 19-21
78. Smits HL, Basahi MA, Díaz R et al. Development and evaluation of a rapid dipstick assay for serodiagnosis of acute human brucellosis. J Clin Microbiol 1999; 37: 4179-82
79. Spink WW, Anderson D. Correlation of a rapid slide-agglutination test (Castaneda) with a tube-agglutination test in screening suspected cases of human brucellosis. J Lab Clin Med 1952; 40: 593-600
80. Spink WW, McCullough NB, Hutchings LM, Mingle CK. A standardized antigen and agglutination technique for human brucellosis. Report No. 3 of the National Research Council, Committee on Public Health Aspects of Brucellosis. Am J Clin Pathol 1954; 24: 496-8
81. Spink WW, McCullough NB, Hutchings LM, Mingle CK. Diagnostic criteria for human brucellosis. Report No. 2 of the National Research Council, Committee on Public Health Aspects of Brucellosis. JAMA 1952; 149: 805-8
82. Spink WW. What is chronic brucellosis? Ann Intern Med 1951; 35: 358-74
83. Stemshorn BW, Forbes LB, Eaglesome MD, Nielsen KH, Robertson FJ, Samagh BS. A comparison of standard serological tests for the diagnosis of bovine brucellosis in Canada. Can J Comp Med 1985; 49: 391-4
84. Tabatabai LB, Deyoe BL. Specific enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus. J Clin Microbiol 1984; 20: 209-13
85. Wilkinson PC. Immunoglobulin patterns of antibodies against Brucella in man and animals. J Immun 1966; 96: 457-63
86. Wilson MM, Merrifield EVO. The anti-globulin (Coombs') test in brucellosis. Lancet 1951; 2: 913-4
87. Young EJ. An overview of human brucellosis. Clin Infect Dis 1995; 21: 283-90
88. Young EJ. Human brucellosis. Rev Infect Dis 1983; 5: 821-42
89. Zinneman HH, Glenchur H, Hall WH. The nature of blocking antibodies in human brucellosis. J Immunol 1959; 83: 206-12