Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

Journal of Clinical Microbiology

Volumn 42, Issue 5, 2004, Pages 2261-2263

  Bogdanovich, T ^a   Skurnik, M ^b   Lubeck P S ^c   Ahrens, P ^c   Hoorfar, J ^c  

^a UNIVERSITY OF TURKU   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR MEDIUM; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BRUCELLA; DIAGNOSTIC VALUE; GENE AMPLIFICATION; GENUS; NONHUMAN; PER GENE; PRIORITY JOURNAL; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; VALIDATION PROCESS; ANIMAL; ANIMAL DISEASE; BRUCELLOSIS; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STATISTICS; VALIDATION STUDY;

BACTERIA (MICROORGANISMS); BRUCELLA; NEGIBACTERIA;

AMINOTRANSFERASE; BACTERIAL DNA; EPIMERASE; PEROSAMINE SYNTHETASE; PRIMER DNA;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; BASE SEQUENCE; BRUCELLA; BRUCELLOSIS; CARBOHYDRATE EPIMERASES; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSAMINASES;

EID: 2442606589     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.5.2261-2263.2004     Document Type: Article

References (16)

1. Abu Al-Soud, W., and P. Rådström. 1998. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl. Environ. Microbiol. 64:3748-3753.
2. Anonymous. 2002. Microbiology of food and animal feeding stuffs - polymerase chain reaction (PCR) for the detection of foodborne pathogens - general method specific requirements, draft international standard ISO/DIS 22174. DIN, Berlin, Germany.
3. Bricker, B. J. 2002. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 90:435-446.
4. Cloeckaert, A., M. Grayon, J.-M. Verger, J.-J. Letesson, and F. Godfroid. 2000. Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp. Res. Microbiol. 151:209-216.
5. Garin-Bastuji, B., N. Hummel, G. Gerbier, C. Cau, R. Pouillot, M. Da Costa, and J. J. Fontaine. 1999. Nonspecific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O:9. Vet. Microbiol. 66:223-233.
6. Godfroid, J., and A. Käsbohrer. 2002. Brucellosis in the European Union and Norway at the turn of the twenty-first century. Vet. Microbiol. 90:135-145.
7. Godfroid, J., C. Saegerman, V. Wellemans, K. Walravens, J. J. Letesson, A. Tibor, A. McMillan, S. Spencer, M. Sanna, D. Bakker, R. Pouillot, and B. Garin-Bastuji. 2002. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet. Microbiol. 90:461-477.
8. Hoorfar, J., P. Ahrens, and P. Rådström. 2000. Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J. Clin. Microbiol. 38:3429-3435.
9. Kittelberger, R., F. Hilbink, M. F. Hansen, G. P. Ross, M. A. Joyce, S. Fenwick, J. Heesemann, H. Wolf-Watz, and K. Nielsen. 1995. Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9. Part II. The use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants. Vet. Microbiol. 47:271-280.
10. Lantz, P. G., B. Hahn-Hägerdal, and P. Rådström. 1994. Sample preparation methods in PCR-based detection of food pathogens. Trends Food Sci. Technol. 5:384-389.
11. Lübeck P. S., M. Skurnik, P. Ahrens, and J. Hoorfar. 2003. A multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on the perosamine synthetase gene, p. 451-453. In M. Skurnik, J. A. Bengoechea, and K. Granfors (ed.), The genus of Yersinia. Kluwer Plenum, New York, N.Y.
12. Lübeck, P. S., P. Wolfs, S. L. W. On, P. Ahrens, P. Rådström, and J. Hoorfar. 2003. Toward an international standard for PCR-based detection of food-borne thermotolerant campylobacters: assay development and analytical validation. Appl. Environ. Microbiol. 69:5664-5669.
13. Malorny, B., J. Hoorfar, M. Hugas, A. Heuvelink, P. Fach, L. Ellerbroek, C. Bunge, C. Dorn, and R. Helmuth. 2003. Inter-laboratory diagnostic accuracy of a Salmonella specific PCR-based method. Int. J. Food Microbiol. 2796: 1-9.
14. Moreno, E., A. Cloeckaert, and I. Moriyon. 2002. Brucella evolution and taxonomy. Vet. Microbiol. 90:209-227.
15. Redkar, R., S. Rose, Bricker, B., and V. DelVecchio. 2001. Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis. Mol. Cell. Probes 1503-52.
16. Weynants, V., A. Tibor, P. A. Denoel, C. Saegerman, J. Godfroid, P. Thiange, and J. J. Letesson. 1996. Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet. Microbiol. 48:101-112.