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Volumn 43, Issue 4, 2007, Pages 427-440

Phase separation in the isolation and purification of membrane proteins (BioTechniques 43, (427-438) DOI 10.2144/000112566);Phase separation in the isolation and purification of membrane proteins

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENTS; PHASE SEPARATION; PURIFICATION;

EID: 35648985642     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112679     Document Type: Erratum
Times cited : (78)

References (58)
  • 1
    • 0001141495 scopus 로고
    • Solubilization and reconstitution of membrane proteins
    • J.B.C. Findlay and W.H. Evans Eds, IRL Press, Washington, DC
    • Jones, O.T., J.P. Earnest, and M.G. McNamee. 1987. Solubilization and reconstitution of membrane proteins, p. 139-177. In J.B.C. Findlay and W.H. Evans (Eds.), Biological Membranes: A Practical Approach. IRL Press, Washington, DC.
    • (1987) Biological Membranes: A Practical Approach , pp. 139-177
    • Jones, O.T.1    Earnest, J.P.2    McNamee, M.G.3
  • 2
    • 0032744963 scopus 로고    scopus 로고
    • A day in the life of Dr. K. or how I learned to stop worrying and love lysozyme: A tragedy in six acts
    • von Heijne, G. 1999. A day in the life of Dr. K. or how I learned to stop worrying and love lysozyme: a tragedy in six acts. J. Mol. Biol. 293:367-379.
    • (1999) J. Mol. Biol , vol.293 , pp. 367-379
    • von Heijne, G.1
  • 3
    • 33745889633 scopus 로고    scopus 로고
    • Efficient and non-denaturing membrane solubilization combined with enrichment of membrane protein complexes by detergent/polymer aqueous two-phase partitioning for proteome analysis
    • Everberg, H., T. Leiding, A. Schioth, F. Tjerneld, and N. Gustavsson. 2006. Efficient and non-denaturing membrane solubilization combined with enrichment of membrane protein complexes by detergent/polymer aqueous two-phase partitioning for proteome analysis. J. Chromatogr. A. 1122:35-46.
    • (2006) J. Chromatogr. A , vol.1122 , pp. 35-46
    • Everberg, H.1    Leiding, T.2    Schioth, A.3    Tjerneld, F.4    Gustavsson, N.5
  • 4
    • 0033842039 scopus 로고    scopus 로고
    • Enrichment of hydrophobic proteins via Triton X-114 phase partitioning and hydroxyapatite column chromatography for mass spectrometry
    • Wissing, J., S. Heim, L. Flohe, U. Bilitewski, and R. Frank. 2000. Enrichment of hydrophobic proteins via Triton X-114 phase partitioning and hydroxyapatite column chromatography for mass spectrometry. Electrophoresis 21:2589-2593.
    • (2000) Electrophoresis , vol.21 , pp. 2589-2593
    • Wissing, J.1    Heim, S.2    Flohe, L.3    Bilitewski, U.4    Frank, R.5
  • 5
    • 33745747870 scopus 로고    scopus 로고
    • Purification of the YadA membrane anchor for secondary structure analysis and crystallization
    • Wollmann, P., K. Zeth, A.N. Lupas, and D. Linke. 2006. Purification of the YadA membrane anchor for secondary structure analysis and crystallization. Int. J. Biol. Macromol. 39:3-9.
    • (2006) Int. J. Biol. Macromol , vol.39 , pp. 3-9
    • Wollmann, P.1    Zeth, K.2    Lupas, A.N.3    Linke, D.4
  • 6
    • 0025211780 scopus 로고
    • Detergents: An overview
    • Neugebauer, J.M. 1990. Detergents: an overview. Methods Enzymol. 182:239-253.
    • (1990) Methods Enzymol , vol.182 , pp. 239-253
    • Neugebauer, J.M.1
  • 7
    • 0016657917 scopus 로고
    • Solubilization of membranes by detergents
    • Helenius, A. and K. Simons. 1975. Solubilization of membranes by detergents. Biochim. Biophys. Acta 415:29-79.
    • (1975) Biochim. Biophys. Acta , vol.415 , pp. 29-79
    • Helenius, A.1    Simons, K.2
  • 8
    • 0035980050 scopus 로고    scopus 로고
    • Detergents as tools in membrane biochemistry
    • Garavito, R.M. and S. Ferguson-Miller. 2001. Detergents as tools in membrane biochemistry. J. Biol. Chem. 276:32403-32406.
    • (2001) J. Biol. Chem , vol.276 , pp. 32403-32406
    • Garavito, R.M.1    Ferguson-Miller, S.2
  • 9
    • 0027305453 scopus 로고
    • Phase separation of nonionic detergents by salt addition and its application to membrane proteins
    • Fricke, B. 1993. Phase separation of nonionic detergents by salt addition and its application to membrane proteins. Anal. Biochem. 212:154-159.
    • (1993) Anal. Biochem , vol.212 , pp. 154-159
    • Fricke, B.1
  • 10
    • 0024979727 scopus 로고
    • Miscibility gap of octyl glucoside-phosphatidylcholine micellar solutions - partition of the nicotinic acetylcholine-receptor into the surfactant-rich phase
    • Schurholz, T., A. Gieselmann, and E. Neumann. 1989. Miscibility gap of octyl glucoside-phosphatidylcholine micellar solutions - partition of the nicotinic acetylcholine-receptor into the surfactant-rich phase. Biochim. Biophys. Acta 986:225-233.
    • (1989) Biochim. Biophys. Acta , vol.986 , pp. 225-233
    • Schurholz, T.1    Gieselmann, A.2    Neumann, E.3
  • 12
    • 0014961076 scopus 로고
    • Studies on aqueous dextran-poly(ethylene glycol) 2-phase systems-containing charged poly(ethylene glycol). I. Partition of albumins
    • Johansson, G. 1970. Studies on aqueous dextran-poly(ethylene glycol) 2-phase systems-containing charged poly(ethylene glycol). I. Partition of albumins. Biochim. Biophys. Acta 222:381-390.
    • (1970) Biochim. Biophys. Acta , vol.222 , pp. 381-390
    • Johansson, G.1
  • 13
    • 0029897576 scopus 로고    scopus 로고
    • Protein partition between the different phases comprising poly(ethylene glycol)-salt aqueous two-phase systems, hydrophobic interaction chromatography and precipitation: A generic description in terms of salting-out effects
    • Huddleston, J., J.C. Abelaira, R.D. Wang, and A. Lyddiatt. 1996. Protein partition between the different phases comprising poly(ethylene glycol)-salt aqueous two-phase systems, hydrophobic interaction chromatography and precipitation: A generic description in terms of salting-out effects. J. Chromatogr. B Biomed. Appl. 680:31-41.
    • (1996) J. Chromatogr. B Biomed. Appl , vol.680 , pp. 31-41
    • Huddleston, J.1    Abelaira, J.C.2    Wang, R.D.3    Lyddiatt, A.4
  • 14
    • 2642531799 scopus 로고    scopus 로고
    • Practical application of aqueous two-phase partition to process development for the recovery of biological products
    • Rito-Palomares, M. 2004. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J. Chromatog. B. Analyt. Technol. Biomed Life Sci. 807:3-11.
    • (2004) J. Chromatog. B. Analyt. Technol. Biomed Life Sci , vol.807 , pp. 3-11
    • Rito-Palomares, M.1
  • 15
    • 0015838754 scopus 로고
    • Application of the phase partition method to a hydrophobic membrane protein, phospholipase A1 from Escherichia coli
    • Albertsson, P.A. 1973. Application of the phase partition method to a hydrophobic membrane protein, phospholipase A1 from Escherichia coli. Biochemistry 12:2525-2530.
    • (1973) Biochemistry , vol.12 , pp. 2525-2530
    • Albertsson, P.A.1
  • 16
    • 0017358560 scopus 로고
    • Use of phase separation in polymers for fractionation of hydrophobic membrane proteins
    • Lukoyanova, M.A. and N.M. Petukhova. 1976. Use of phase separation in polymers for fractionation of hydrophobic membrane proteins. Biochemistry (Mosc.) 41:1468-1475.
    • (1976) Biochemistry (Mosc.) , vol.41 , pp. 1468-1475
    • Lukoyanova, M.A.1    Petukhova, N.M.2
  • 17
    • 0034611647 scopus 로고    scopus 로고
    • Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins
    • Sivars, U. and F. Tjerneld. 2000. Mechanisms of phase behaviour and protein partitioning in detergent/polymer aqueous two-phase systems for purification of integral membrane proteins. Biochim. Biophys. Acta 1474:133-146.
    • (2000) Biochim. Biophys. Acta , vol.1474 , pp. 133-146
    • Sivars, U.1    Tjerneld, F.2
  • 18
    • 0037081755 scopus 로고    scopus 로고
    • A novel two-step extraction method with detergent/polymer systems for primary recovery of the fusion protein endo-glucanase I-hydrophobin I
    • Collen, A., J. Persson, M. Linder, T. Nakari-Setala, M. Penttila, F. Tjerneld, and U. Sivars. 2002. A novel two-step extraction method with detergent/polymer systems for primary recovery of the fusion protein endo-glucanase I-hydrophobin I. Biochim. Biophys. Acta 1569:139-150.
    • (2002) Biochim. Biophys. Acta , vol.1569 , pp. 139-150
    • Collen, A.1    Persson, J.2    Linder, M.3    Nakari-Setala, T.4    Penttila, M.5    Tjerneld, F.6    Sivars, U.7
  • 19
    • 1542653049 scopus 로고    scopus 로고
    • Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems
    • Selber, K., F. Tjerneld, A. Collen, T. Hyytia, T. Nakari-Setala, M. Bailey, R. Fagerstrom, J. Kan, et al. 2004. Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems. Process Biochem. 39:889-896.
    • (2004) Process Biochem , vol.39 , pp. 889-896
    • Selber, K.1    Tjerneld, F.2    Collen, A.3    Hyytia, T.4    Nakari-Setala, T.5    Bailey, M.6    Fagerstrom, R.7    Kan, J.8
  • 20
    • 0033231204 scopus 로고    scopus 로고
    • Surfactant-mediated cloud point extractions: An environmentally benign alternative separation approach
    • Quina, F.H. and W.L. Hinze. 1999. Surfactant-mediated cloud point extractions: An environmentally benign alternative separation approach. Ind. Eng. Chem. Res. 38:4150-4168.
    • (1999) Ind. Eng. Chem. Res , vol.38 , pp. 4150-4168
    • Quina, F.H.1    Hinze, W.L.2
  • 22
    • 0028060303 scopus 로고
    • Phase separation of biomolecules in polyoxyethylene glycol non-ionic detergents
    • Sanchez-Ferrer, A., R. Bru, and F. Garcia-Carmona. 1994. Phase separation of biomolecules in polyoxyethylene glycol non-ionic detergents. Crit. Rev. Biochem. Mol. Biol. 29:275-313.
    • (1994) Crit. Rev. Biochem. Mol. Biol , vol.29 , pp. 275-313
    • Sanchez-Ferrer, A.1    Bru, R.2    Garcia-Carmona, F.3
  • 23
    • 0019887744 scopus 로고
    • Phase separation of integral membrane proteins in Triton X-114 solution
    • Bordier, C. 1981. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 256:1604-1607.
    • (1981) J. Biol. Chem , vol.256 , pp. 1604-1607
    • Bordier, C.1
  • 24
    • 0032286098 scopus 로고    scopus 로고
    • Aqueous micellar two-phase systems for protein separation
    • Tani, H., T. Kamidate, and H. Watanabe. 1998. Aqueous micellar two-phase systems for protein separation. Anal. Sci. 14:875-888.
    • (1998) Anal. Sci , vol.14 , pp. 875-888
    • Tani, H.1    Kamidate, T.2    Watanabe, H.3
  • 25
    • 0025878583 scopus 로고
    • Glycerol allows low-temperature phase separation of membrane proteins solubilized in Triton X-114: Application to the purification of plant cytochromes P-450 and b5
    • Werck-Reichhart, D., I. Benveniste, H. Teutsch, F. Durst, and B. Gabriac. 1991. Glycerol allows low-temperature phase separation of membrane proteins solubilized in Triton X-114: application to the purification of plant cytochromes P-450 and b5. Anal. Biochem. 197:125-131.
    • (1991) Anal. Biochem , vol.197 , pp. 125-131
    • Werck-Reichhart, D.1    Benveniste, I.2    Teutsch, H.3    Durst, F.4    Gabriac, B.5
  • 27
    • 2542456010 scopus 로고    scopus 로고
    • Salt effects on surfactant aggregation and dye-micelle complexation
    • Acharya, K.R., S.C. Bhattacharya, and S.P. Moulik. 1997. Salt effects on surfactant aggregation and dye-micelle complexation. Ind. J. Chem. A. 36:137-143.
    • (1997) Ind. J. Chem. A , vol.36 , pp. 137-143
    • Acharya, K.R.1    Bhattacharya, S.C.2    Moulik, S.P.3
  • 28
    • 0032568988 scopus 로고    scopus 로고
    • Purification of recombinant apolipoprotein A-1(Milano) expressed in Escherichia coli using aqueous two-phase extraction followed by temperature-induced phase separation
    • Persson, J., L. Nystrom, H. Ageland, and F. Tjerneld. 1998. Purification of recombinant apolipoprotein A-1(Milano) expressed in Escherichia coli using aqueous two-phase extraction followed by temperature-induced phase separation. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 711:97-109.
    • (1998) J. Chromatogr. B Analyt. Technol. Biomed. Life Sci , vol.711 , pp. 97-109
    • Persson, J.1    Nystrom, L.2    Ageland, H.3    Tjerneld, F.4
  • 29
    • 0033589830 scopus 로고    scopus 로고
    • Purification of recombinant and human apolipoprotein A-1 using surfactant micelles in aqueous two-phase systems: Recycling of thermoseparating polymer and surfactant with temperature-induced phase separation
    • Persson, J., L. Nystrom, H. Ageland, and F. Tjerneld. 1999. Purification of recombinant and human apolipoprotein A-1 using surfactant micelles in aqueous two-phase systems: recycling of thermoseparating polymer and surfactant with temperature-induced phase separation. Biotechnol. Bioeng. 65:371-381.
    • (1999) Biotechnol. Bioeng , vol.65 , pp. 371-381
    • Persson, J.1    Nystrom, L.2    Ageland, H.3    Tjerneld, F.4
  • 30
    • 0027830276 scopus 로고
    • A critical-review of surfactant-mediated phase separations (cloud-point extractions) - theory and applications
    • Hinze, W.L. and E. Pramauro. 1993. A critical-review of surfactant-mediated phase separations (cloud-point extractions) - theory and applications. Crit. Rev. Anal. Chem. 24:133-177.
    • (1993) Crit. Rev. Anal. Chem , vol.24 , pp. 133-177
    • Hinze, W.L.1    Pramauro, E.2
  • 31
    • 19844371602 scopus 로고
    • Phase-behavior of polyoxyethylene surfactants with water - mesophase structures and partial miscibility (cloud points)
    • Mitchell, D.J., G.J.T. Tiddy, L. Waring, T. Bostock, and M.P. Mcdonald. 1983. Phase-behavior of polyoxyethylene surfactants with water - mesophase structures and partial miscibility (cloud points). J. Chem. Soc. Faraday Trans I 79:975-1000.
    • (1983) J. Chem. Soc. Faraday Trans I , vol.79 , pp. 975-1000
    • Mitchell, D.J.1    Tiddy, G.J.T.2    Waring, L.3    Bostock, T.4    Mcdonald, M.P.5
  • 33
    • 0030572631 scopus 로고    scopus 로고
    • Phase behavior of nonionic surfactant solutions in the presence of polyvinylpyrrolidone
    • Pandit, N.K. and J. Kanjia. 1996. Phase behavior of nonionic surfactant solutions in the presence of polyvinylpyrrolidone. Int. J. Pharm. 141:197-203.
    • (1996) Int. J. Pharm , vol.141 , pp. 197-203
    • Pandit, N.K.1    Kanjia, J.2
  • 34
    • 33845379843 scopus 로고
    • Surface-properties and micellar interfacial microenvironment of n-dodecyl beta-D-maltoside
    • Drummond, C.J., G.G. Warr, F. Grieser, B.W. Ninham, and D.F. Evans. 1985. Surface-properties and micellar interfacial microenvironment of n-dodecyl beta-D-maltoside. J. Phys. Chem. 89:2103-2109.
    • (1985) J. Phys. Chem , vol.89 , pp. 2103-2109
    • Drummond, C.J.1    Warr, G.G.2    Grieser, F.3    Ninham, B.W.4    Evans, D.F.5
  • 35
    • 0000571611 scopus 로고
    • The growth and characterization of membrane-protein crystals
    • Garavito, R.M., Z. Markovichousley, and J.A. Jenkins. 1986. The growth and characterization of membrane-protein crystals. J. Cryst. Growth 76:701-709.
    • (1986) J. Cryst. Growth , vol.76 , pp. 701-709
    • Garavito, R.M.1    Markovichousley, Z.2    Jenkins, J.A.3
  • 37
    • 84998439402 scopus 로고
    • Polymer-induced phase-separation in aqueous micellar solutions of alkylglucosides for protein extraction
    • Saitoh, T., H. Tani, T. Kamidate, T. Kamataki, and H. Watanabe. 1994. Polymer-induced phase-separation in aqueous micellar solutions of alkylglucosides for protein extraction. Anal. Sci. 10:299-303.
    • (1994) Anal. Sci , vol.10 , pp. 299-303
    • Saitoh, T.1    Tani, H.2    Kamidate, T.3    Kamataki, T.4    Watanabe, H.5
  • 38
    • 0031555171 scopus 로고    scopus 로고
    • Polymer-induced phase separation in aqueous micellar solutions of octyl-beta-D-thioglucoside for extraction of membrane proteins
    • Tani, H., T. Saitoh, T. Kamidate, T. Kamataki, and H. Watanabe. 1997. Polymer-induced phase separation in aqueous micellar solutions of octyl-beta-D-thioglucoside for extraction of membrane proteins. Biotechnol. Bioeng. 56:311-318.
    • (1997) Biotechnol. Bioeng , vol.56 , pp. 311-318
    • Tani, H.1    Saitoh, T.2    Kamidate, T.3    Kamataki, T.4    Watanabe, H.5
  • 39
    • 2042546070 scopus 로고    scopus 로고
    • Spontaneous emulsification of detergent solubilized reaction center: Protein conformational changes precede droplet growth
    • Palazzo, G., A. Mallardi, F. Francia, M. Dezi, G. Venturoli, M. Pierno, E. Vignati, and R. Piazza. 2004. Spontaneous emulsification of detergent solubilized reaction center: protein conformational changes precede droplet growth. Phys. Chem. Chem. Phys. 6:1439-1445.
    • (2004) Phys. Chem. Chem. Phys , vol.6 , pp. 1439-1445
    • Palazzo, G.1    Mallardi, A.2    Francia, F.3    Dezi, M.4    Venturoli, G.5    Pierno, M.6    Vignati, E.7    Piazza, R.8
  • 41
    • 0023791470 scopus 로고
    • Use of digitonin-permeabilized adipocytes for cAMP studies
    • Mooney, R.A. 1988. Use of digitonin-permeabilized adipocytes for cAMP studies. Methods Enzymol. 159:193-202.
    • (1988) Methods Enzymol , vol.159 , pp. 193-202
    • Mooney, R.A.1
  • 42
    • 0030175017 scopus 로고    scopus 로고
    • A mild purification method for polysaccharide binding membrane proteins: Phase separation of digitonin extracts to isolate the hyaluronate synthase from Streptococcus sp in active form
    • Prehm, S., V. Nickel, and P. Prehm. 1996. A mild purification method for polysaccharide binding membrane proteins: phase separation of digitonin extracts to isolate the hyaluronate synthase from Streptococcus sp in active form. Protein Expr. Purif. 7:343-346.
    • (1996) Protein Expr. Purif , vol.7 , pp. 343-346
    • Prehm, S.1    Nickel, V.2    Prehm, P.3
  • 43
    • 0020210815 scopus 로고
    • N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry
    • Hildreth, J.E.K. 1982. N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem. J. 207:363-366.
    • (1982) Biochem. J , vol.207 , pp. 363-366
    • Hildreth, J.E.K.1
  • 44
    • 0000315631 scopus 로고
    • The upper consolute boundary in zwitterionic surfactant-water systems
    • Nilsson, P.G., B. Lindman, and R.G. Laughlin. 1984. The upper consolute boundary in zwitterionic surfactant-water systems. J. Phys. Chem. 88:6357-6362.
    • (1984) J. Phys. Chem , vol.88 , pp. 6357-6362
    • Nilsson, P.G.1    Lindman, B.2    Laughlin, R.G.3
  • 45
    • 0026332021 scopus 로고
    • Concentration of hydrophobic organic-compounds and extraction of protein using alkylammoniosulfate zwitterionic surfactant mediated phase separations (cloud point extractions)
    • Saitoh, T. and W.L. Hinze. 1991. Concentration of hydrophobic organic-compounds and extraction of protein using alkylammoniosulfate zwitterionic surfactant mediated phase separations (cloud point extractions). Anal. Chem. 63:2520-2525.
    • (1991) Anal. Chem , vol.63 , pp. 2520-2525
    • Saitoh, T.1    Hinze, W.L.2
  • 46
    • 0018127607 scopus 로고
    • Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants
    • Gonenne, A. and R. Ernst. 1978. Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants. Anal. Biochem. 87:28-38.
    • (1978) Anal. Biochem , vol.87 , pp. 28-38
    • Gonenne, A.1    Ernst, R.2
  • 47
    • 0019082582 scopus 로고
    • A non-denaturing zwitterionic detergent for membrane biochemistry - design and synthesis
    • Hjelmeland, L.M. 1980. A non-denaturing zwitterionic detergent for membrane biochemistry - design and synthesis. Proc. Natl. Acad. Sci. USA 77:6368-6370.
    • (1980) Proc. Natl. Acad. Sci. USA , vol.77 , pp. 6368-6370
    • Hjelmeland, L.M.1
  • 48
    • 0001002965 scopus 로고
    • The effect of salt identity and concentration on liquid liquid-phase separation in aqueous micellar solutions of C-8-lecithin
    • Huang, Y.X., G.M. Thurston, D. Blankschtein, and G.B. Benedek. 1990. The effect of salt identity and concentration on liquid liquid-phase separation in aqueous micellar solutions of C-8-lecithin. J. Chem. Phys. 92:1956-1962.
    • (1990) J. Chem. Phys , vol.92 , pp. 1956-1962
    • Huang, Y.X.1    Thurston, G.M.2    Blankschtein, D.3    Benedek, G.B.4
  • 50
    • 0001115559 scopus 로고
    • Observation of polymerlike phase separation of protein-surfactant complexes in solution
    • Guo, X.-H. and S.-H. Chen. 1990. Observation of polymerlike phase separation of protein-surfactant complexes in solution. Phys. Rev. Lett. 64:1979.
    • (1990) Phys. Rev. Lett , vol.64 , pp. 1979
    • Guo, X.-H.1    Chen, S.-H.2
  • 52
    • 0016157537 scopus 로고
    • Interaction of a cationic detergent with bovine serum-albumin and other proteins
    • Nozaki, Y., J.A. Reynolds, and C. Tanford. 1974. Interaction of a cationic detergent with bovine serum-albumin and other proteins. J. Biol. Chem. 249:4452-4459.
    • (1974) J. Biol. Chem , vol.249 , pp. 4452-4459
    • Nozaki, Y.1    Reynolds, J.A.2    Tanford, C.3
  • 53
    • 0015396799 scopus 로고
    • Preparation and properties of phospholipid bilayers containing rhodopsin
    • Hong, K. and W.L. Hubbell. 1972. Preparation and properties of phospholipid bilayers containing rhodopsin. Proc. Natl. Acad. Sci. USA 69:2617-2621.
    • (1972) Proc. Natl. Acad. Sci. USA , vol.69 , pp. 2617-2621
    • Hong, K.1    Hubbell, W.L.2
  • 54
    • 0037105647 scopus 로고    scopus 로고
    • Cloud-point extraction and preconcentration of cyanobacterial toxins (microcystins) from natural waters using a cationic surfactant
    • Man, B.K.W., M.H.W. Lam, P.K.S. Lam, R.S.S. Wu, and G. Shaw. 2002. Cloud-point extraction and preconcentration of cyanobacterial toxins (microcystins) from natural waters using a cationic surfactant. Environ. Sci. Technol. 36:3985-3990.
    • (2002) Environ. Sci. Technol , vol.36 , pp. 3985-3990
    • Man, B.K.W.1    Lam, M.H.W.2    Lam, P.K.S.3    Wu, R.S.S.4    Shaw, G.5
  • 55
    • 4043184346 scopus 로고    scopus 로고
    • Two-phase aqueous micellar systems - an alternative method for protein purification
    • Rangel-Yagui, C.O., A. Pessoa-Jr, and D. Blankschtein. 2004. Two-phase aqueous micellar systems - an alternative method for protein purification. Braz. J. Chem. Eng. 21:531-544.
    • (2004) Braz. J. Chem. Eng , vol.21 , pp. 531-544
    • Rangel-Yagui, C.O.1    Pessoa-Jr, A.2    Blankschtein, D.3
  • 56
    • 0025787077 scopus 로고
    • Resolution of rhodocyclus-gelatinosus photoreceptor unit components by temperature-induced phase-separation in the presence of decyltetraoxyethylene
    • Agalidis, H. and F. Reisshusson. 1991. Resolution of rhodocyclus-gelatinosus photoreceptor unit components by temperature-induced phase-separation in the presence of decyltetraoxyethylene. Biochem. Biophys. Res. Commun. 177:1107-1112.
    • (1991) Biochem. Biophys. Res. Commun , vol.177 , pp. 1107-1112
    • Agalidis, H.1    Reisshusson, F.2
  • 57
    • 0026289269 scopus 로고
    • The partitioning of cholesterol oxidase in Triton X-114-based aqueous two-phase systems
    • Ramelmeier, R.A., G.C. Terstappen, and M.R. Kula. 1991. The partitioning of cholesterol oxidase in Triton X-114-based aqueous two-phase systems. Bioseparation 2:315-324.
    • (1991) Bioseparation , vol.2 , pp. 315-324
    • Ramelmeier, R.A.1    Terstappen, G.C.2    Kula, M.R.3
  • 58
    • 0034446067 scopus 로고    scopus 로고
    • Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems
    • Rodenbrock, A., K. Selber, M.R. Egmond, and M.R. Kula. 2000. Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems. Bioseparation 9:269-276.
    • (2000) Bioseparation , vol.9 , pp. 269-276
    • Rodenbrock, A.1    Selber, K.2    Egmond, M.R.3    Kula, M.R.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.