Enrichment of hydrophobic proteins via Triton X-114 phase partitioning and hydroxyapatite column chromatography for mass spectrometry

Electrophoresis

Volumn 21, Issue 13, 2000, Pages 2589-2593

  Wissing, Josef ^a   Heim, Sabina ^a   Flohé, Leopold ^a   Bilitewski, Ursula ^b   Frank, Ronald ^b  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Hydroxyapatite; Mass spectrometry; Membrane proteins; Triton X 114; Two dimensional electrophoresis

Indexed keywords

BIOLOGICAL MEMBRANES; CELL CULTURE; COLUMN CHROMATOGRAPHY; ELECTROPHORESIS; ENDOTHELIAL CELLS; HYDROPHOBICITY; HYDROXYAPATITE; PHASE SEPARATION; PROTEINS; SIGNAL TRANSDUCTION;

HYDROPHOBIC PROTEIN; MEMBRANE PROTEINS; PHASE PARTITIONING; PROTEOME ANALYSIS; SEPARATION AND IDENTIFICATION; SIGNAL TRANSDUCTION PATHWAYS; SPECIFIC PROPERTIES; TRITON X 114; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL SEPARATIONS;

MASS SPECTROMETRY;

MEMBRANE PROTEIN;

ARTICLE; COLUMN CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYDROPHOBICITY; MASS SPECTROMETRY; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION;

EID: 0033842039     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/1522-2683(20000701)21:13<2589::AID-ELPS2589>3.0.CO;2-T     Document Type: Article

References (14)