Eukaryotic integral membrane protein expression utilizing the Escherichia coli glycerol-conducting channel protein (GlpF)

Applied Microbiology and Biotechnology

Volumn 77, Issue 2, 2007, Pages 375-381

  Neophytou, Irene ^a   Harvey, Richard ^a   Lawrence, Jayne ^a   Marsh, Phil ^a   Panaretou, Barry ^a   Barlow, David ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

Escherichia coli; Gene expression; Membrane protein; Protein engineering; Recombinant protein

Indexed keywords

MEMBRANE PROTEIN; METAL AFFINITY CHROMATOGRAPHY; PROTEIN ENGINEERING; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; BIOLOGICAL MEMBRANES; ESCHERICHIA COLI; GENE EXPRESSION; GLYCEROL;

PROTEINS;

BACTERIAL PROTEIN; CLAUDIN 4; GLYCEROL; GLYCEROL CONDUCTING CHANNEL PROTEIN; HEXAHISTIDINE; INWARDLY RECTIFYING POTASSIUM CHANNEL; MEMBRANE PROTEIN; OCCLUDIN; OXIDOREDUCTASE; PEPTIDASE; UNCLASSIFIED DRUG;

BACTERIUM; EUKARYOTE; GENE EXPRESSION; MEMBRANE; PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIAL MEMBRANE; BACTERIUM CULTURE; CYTOLYSIS; ESCHERICHIA COLI; EUKARYOTE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AQUAPORINS; CLONING, MOLECULAR; DUODENUM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EUKARYOTIC CELLS; FMN REDUCTASE; HUMANS; MEMBRANE PROTEINS; POTASSIUM CHANNELS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; EUKARYOTA;

EID: 35648948931     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1174-7     Document Type: Article

References (30)

1. Ando-Akatsuka Y, Saitou M, Hirase T, Kishi M, Sakakibara A, Itoh M, Yonemura S, Furuse M, Tsukita S (1996) Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J Cell Biol 133:43-47
2. + channel from human heart and brain: cloning and stable expression in a human cell line. Am J Physiol 268:506-511
3. Bibi E, Gros P, Kaback HR (1993) Functional expression of mouse mdr1 in Escherichia coli. Proc Natl Acad Sci U S A 90:9209-9213
4. + conduction and selectivity. Science 280:69-77
5. Drew DE, Heijne G, Nordlund P, de Gier JW (2001) Green fluorescent protein as an indicator to monitor membrane protein over-expression in Escherichia coli. FEBS Lett 507:220-224
6. Erni B, Zanolari B (1986) Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc. J Biol Chem 261:16398-16403
7. Fiermonte G, Palmieri L, Todisco S, Agrimi G, Palmieri F, Walker JE (2002) Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms. J Biol Chem 272:19289-19294
8. Fu D, Libson A, Miercke LJW, Weitzman C, Nollert P, Krucinski J, Stroud RM (2000) Structure of a glycerol conducting channel and the basis for its selectivity. Science 290:481-486
9. Guan C, Li P, Riggs PD, Inouye H (1988) Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30
10. Hofmann K, Stoffel W (1993) Tmbase-A database of membrane spanning protein segments. Biol Chem Hoppe-Seyler 374:166
11. Jung Y, Kwak J, Lee Y (2001) High-level production of heme-containing holoproteins in Escherichia coli. Appl Microbiol Biotechnol 55:187-191
12. Katahira J, Inoue N, Horiguchi Y, Matsuda M, Sugimoto N (1997) Molecular cloning and functional characterization of the receptor for Clostridium perfringens enterotoxin. J Cell Biol 136:1239-1247
13. Kobe B, Center RJ, Kemp BE, Poumbourios P (1999) Crystal structure of human T cell leukemia virus type 1 gp21 ectodomain crystallized as a maltose-binding protein chimera reveals structural evolution of retroviral transmembrane proteins. Proc Natl Acad Sci U S A 96:4319-4324
14. Laage R, Langosch D (2001) Strategies for prokaryotic expression of eukaryotic membrane proteins. Traffic 2:99-104
15. Liu Y, Manna A, li R, Martin WE, Murphy RC, Cheung AL, Zhang G (2001) Crystal structure of the SarR protein from Staphylococcus aureus. Proc Natl Acad Sci U S A 98:6877-6882
16. Luirink J, Heijne G, Houben E, de Gier JW (2005) Biogenesis of inner membrane proteins in Escherichia coli. Annu Rev Microbiol 59:329-355
17. Manley DM, McComb ME, Perreault H, Donald LJ, Duckworth HW, O'Neil JD (2000) Secondary structure and oligomerization of the E. coli glycerol facilitator. Biochem 39:12303-12311
18. McKie AT, Barrow D, Latunde-Dada GO, Rolfs A, Sager G, Mudaly E, Mudaly M, Richardson C, Barlow D, Bomford A, Peters TJ, Raja KB, Shirali S, Hediger MA, Farzaneh F, Simpson RJ (2001) An iron-regulated ferric reductase associated with the absorption of dietary iron. Science 291:1755-1759
19. Menke JG, Borkowski JA, Bierilo KK, MacNeil T, Derrick AW, Schneck KA, Ransom RW, Strader CD, Linemeyer DL, Hess JF (1994) Expression cloning of a human B1 bradykinin receptor. J Biol Chem 269:21583-21586
20. Miroux B, Walker JE (1996) Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260:289-298
21. Nakamuta M, Takayanagi R, Sakai Y, Sakamoto S, Hagiwara H, Mizuno T, Saito Y, Hirose S, Yamamoto M, Nawata H (1991) Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor. Biochem Biophys Res Commun 177:34-39
22. Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW (2006) Barnase fusion as a tool to determine the crystal structure of the small disulfide rich protein McoEeTI. J Mol Biol 356:1-8
23. Quick M, Wright EM (2002) Employing Escherichia coli to functionally express, purify, and characterize a human transporter. Proc Natl Acad Sci U S A 99:8597-8601
24. Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S (2005) NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307:1317-1321
25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
26. Smith DB, Johnson KS (1988) Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40
27. Tate CG (2001) Over-expression of mammalian integral membrane proteins for structural studies. FEBS Lett 504:94-98
28. Tjaden J, Schwoppe C, Mohlmann T, Quick P, Neuhaus H (1998) Expression of a plastidic ATP/ADP transporter gene in Escherichia coli leads to a functional adenine nucleotide transport system in the bacterial cytoplasmic membrane. J Biol Chem 273:9630-9636
29. 15NH(3). Biochem J 363:243-252
30. Wilson ML, Macnab RM (1988) Overproduction of the MotA protein of Escherichia coli and estimation of its wild-type level. J Bacteriol 170:588-597