Modular assembly of glycoproteins: Towards the synthesis of GlyCAM-1 by using expressed protein ligation

Angewandte Chemie - International Edition

Volumn 43, Issue 11, 2004, Pages 1355-1359

  Macmillan, Derek ^a   Bertozzi, Carolyn R ^b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Expressed protein ligation; Glycopeptides; Glycoproteins; Protein engineering; Semisynthesis

Indexed keywords

BACTERIA; POLYSACCHARIDES; SYNTHESIS (CHEMICAL);

GLYCOPROTEINS; LIGATION; MODULAR ASSEMBLY;

PROTEINS;

CARBOHYDRATE; GLYCOPROTEIN; PROTEIN GLYCAM 1; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM; METHODOLOGY; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN VARIANT;

AMINO ACID SEQUENCE; GLYCOPEPTIDES; GLYCOPROTEINS; GLYCOSYLATION; LIGANDS; MOLECULAR SEQUENCE DATA; MUCINS; PROTEIN STRUCTURE, TERTIARY;

EID: 3543108674     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200352673     Document Type: Article

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29. Both proteins migrate with a higher apparent molecular mass than predicted, indeed almost twice that determined by mass spectrometry (full-length bacterially derived GlyCAM-1, observed mass (ESI-MS)=14409Da and 14451Da for the acetylated species; semisynthetic GlyCAM-1 observed molecular mass (ESI-MS) = 16776 Da).