Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen species

Journal of Cell Biology

Volumn 166, Issue 3, 2004, Pages 311-316

  Wysocki, Robert ^a   Kron, Stephen J ^a  

^a UNIVERSITY OF CHICAGO   (United States)

Author keywords

Apoptosis; CDC13; FITC VAD FMK; Saccharomyces cerevisiae; YCA1

Indexed keywords

CASPASE; CELL DNA; FLUOROCHROME; GENE PRODUCT; PROTEIN CDC13; PROTEIN YCA1; REACTIVE OXYGEN METABOLITE; TELOMERE BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL CYCLE G2 PHASE; CELL DEATH; CELL LABELING; CELL SIZE; CELL VIABILITY; CHROMOSOME STRUCTURE; CONTROLLED STUDY; CYTOLYSIS; DNA DAMAGE; ENZYME ACTIVATION; FUNGAL CELL; FUNGUS GROWTH; GENE ACTIVITY; GENE INACTIVATION; MITOSIS INHIBITION; MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; YEAST;

APOPTOSIS; CASPASES; CELL CYCLE; DNA DAMAGE; FLUORESCENT DYES; REACTIVE OXYGEN SPECIES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TELOMERE-BINDING PROTEINS;

FUNGI; MAMMALIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 3543068246     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200405016     Document Type: Article

References (31)

1. Abudugupur, A., K. Mitsui, S. Yokota, and K. Tsurugi. 2002. An ARL1 mutation affected autophagic cell death in yeast, causing a defect in central vacuole formation. Cell Death Differ. 9:158-168.
2. Bursch, W. 2001. The autophagosomal-lysosomal compartment in programmed cell death. Cell Death Differ. 8:569-581.
3. Camougrand, N., A. Grelaud-Coq, E. Marza, M. Priault, J.J. Bessoule, and S. Manon. 2003. The product of the UTH1 gene, required for Bax-induced cell death in yeast, is involved in the response to rapamycin. Mol. Microbiol. 47:495-506.
4. Chen, S.R., D.D. Dunigan, and M.B. Dickman. 2003. Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae. Free Radic. Biol. Med. 34:1315-1325.
5. Booth, C., E. Griffith, G. Brady, and D. Lydall. 2001. Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction. Nucleic Acids Res. 29:4414-4422.
6. DuBois, M.L., Z.W. Haimberger, M.W. McIntosh, and D.E. Gottschling. 2002. A quantitative assay for telomere protection in Saccharomyces cerevisiae. Genetics. 161:995-1013.
7. Earnshaw, W.C., L.M. Martins, and S.H. Kaufmann. 1999. Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68:383-424.
8. Garvik, B., M. Carson, and L. Hartwell. 1995. Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Mol. Cell. Biol. 15:6128-6138.
9. Grabarek, J., and Z. Darzynkiewicz. 2002. In situ activation of caspases and serine ptoteases during apoptosis detected by affinity labeling their enzyme active centers with fluorochrome-tagged inhibitors. Exp. Hematol. 30:982-989.
10. Heinisch, J.J., A. Lorberg, H.P. Schmitz, and J.J. Jacoby. 1999. The protein kinase C-mediated MAP kinase pathway involved in the maintenance of cellular integrity in Saccharomyces cerevisiae. Mol. Microbiol. 32:671-680.
11. Henderson, L.M., and J.B. Chappell. 1993. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur. J. Biochem. 217:973-980.
12. Herker, E., H. Jungwirth, K.A. Lehmann, C. Maldener, K.U. Fröhlich, S. Wissing, S. Büttner, M. Fehr, S. Sigrist, and F. Madeo. 2004. Chronological aging leads to apoptosis in yeast. J. Cell Biol. 164:501-507.
13. Fleury, C., B. Mignotte, and J.L. Vayssiere. 2002. Mitochondrial reactive oxygen species in cell death signaling. Biochimie. 84:131-141.
14. Jayaraman, S. 2003. Intracellular determination of activated caspases (IDAC) by flow cytometry using a pancaspase inhibitor labeled with FITC. Cytometry. 56A:104-112.
15. Jia, X., T. Weinert, and D. Lydall. 2004. Mec1 and Rad53 inhibit formation of single-stranded DNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants. Genetics. 166:753-764.
16. Kannan, K., and S.K. Jain. 2000. Oxidative stress and apoptosis. Pathophysiology. 7:153-163.
17. Lee, S.E., J.K. Moore, A. Holmes, K. Umezu, R.D. Kolodner, and J.E. Haber. 1998. Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell. 94:399-409.
18. Lydall, D. 2003. Hiding at the ends of yeast chromosomes: telomeres, nucleases and checkpoint pathways. J. Cell Sci. 116:4057-4065.
19. Madeo, F., E. Fröhlich, M. Ligr, M. Grey, S.J. Sigrist, D.H. Wolf, and K.U. Fröhlich. 1999. Oxygen stress: a regulator of apoptosis in yeast. J. Cell Biol. 145:757-767.
20. Madeo, F., E. Herker, C. Maldener, S. Wissing, S. Lächelt, M. Herlan, M. Fehr, K. Lauber, S.J. Sigrist, S. Wesselborg, and K.U. Fröhlich. 2002a. A caspase-related protease regulates apoptosis in yeast. Mol. Cell. 9:911-917.
21. Madeo, F., S. Engelhardt, E. Herker, N. Lehmann, C. Maldener, A. Proksch, S. Wissing, and K.U. Fröhlich. 2002b. Apoptosis in yeast: a new model system with applications in cell biology and medicine. Curr. Genet. 41:208-216.
22. Maringele, L., and D. Lydall. 2002. EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Δ mutants. Genes Dev. 16:1919-1933.
23. Motizuki, M., S. Yokota, and K. Tsurugi. 1995. Autophagic death after cell cycle arrest at the restrictive temperature in temperature-sensitive cell division cycle and secretory mutants of the yeast Saccharomyces cerevisiae. Eur. J. Cell Biol. 68:275-287.
24. Nugent, C.I., T.R. Hughes, N.F. Lue, and V. Lundblad. 1996. Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science. 274:249-252.
25. L prevents the cytotoxic effect of Bax, but not Bax-induced formation of reactive oxygen species, in Kluyveromyces lactis. Microbiology. 148:2789-2795.
26. Pozarowski, P., X. Huang, D.H. Halicka, B. Lee, G. Johnson, and Z. Darzynkiewicz. 2003. Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells: a caution in data interpretation. Cytometry. 55A:50-60.
27. Qi, H., T.S. Li, D. Kuo, A. Nur-E-Kamal, and L.F. Liu. 2003. Inactivation of Cdc13p triggers MEC1-dependent apoptotic signals in yeast. J. Biol. Chem. 278:15136-15141.
28. L complements Saccharomyces cerevisiae genes that facilitate the switch from glycolytic to oxidative metabolism. J. Biol. Chem. 277:44870-44876.
29. Wadskog, M., C. Maldener, A. Proksch, F. Madeo, and L. Adler. 2004. Yeast lacking the SRO7/SOP1-encoded tumor suppressor homologue show increased susceptibility to apoptosis-like cell death on exposure to NaCl stress. Mol. Biol. Cell. 15:1436-1444.
30. Weinert, T.A., and L.H. Hartwell. 1993. Cell cycle arrest of cdc mutants and specificity of the RAD9 checkpoint. Genetics. 134:63-80.
31. Zhao, J., and G.H. Fleet. 2003. Degradation of DNA during the autolysis of Saccharomyces cerevisiae. J. Ind Microbiol. Biotechnol. 30:175-182.