Crystal structure of a Rad51 filament

Nature Structural and Molecular Biology

Volumn 11, Issue 8, 2004, Pages 791-796

  Conway, Adam B ^a   Lynch, Thomas W ^a   Zhang, Ying ^a   Fortin, Gary S ^b,c   Fung, Cindy W ^b   Symington, Lorraine S ^b   Rice, Phoebe A ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

^c FOX CHASE CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DIMER; RAD51 PROTEIN; RECOMBINASE;

ARTICLE; CRYSTAL STRUCTURE; DELETION MUTANT; DNA BINDING; DNA DAMAGE; DNA REPAIR; DNA STRAND; GENETIC STABILITY; GENETIC VARIABILITY; PRIORITY JOURNAL; PROKARYOTE; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; CRYSTALLOGRAPHY, X-RAY; DNA; DNA DAMAGE; DNA-BINDING PROTEINS; HISTIDINE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MODELS, MOLECULAR; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN CONFORMATION; RAD51 RECOMBINASE; REC A RECOMBINASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TIME FACTORS; TYROSINE;

EUKARYOTA; PROKARYOTA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 3543041245     PISSN: 15459993     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsmb795     Document Type: Article

References (39)

1. Shinohara, A., Ogawa, H. & Ogawa, T. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69, 457-470 (1992).
2. West, S.C. Molecular views of recombination proteins and their control. Nat. Rev. Mol. Cell. Biol. 4, 435-445 (2003).
3. Morgan, E.A., Shah, N. & Symington, L.S. The requirement for ATP hydrolysis by Saccharomyces cerevisiae Rad51 is bypassed by mating-type heterozygosity or RAD54 in high copy. Mol. Cell. Biol. 22, 6336-6343 (2002).
4. Sung, P. & Stratton, S.A. Yeast Rad51 recombinase mediates polar DNA strand exchange in the absence of ATP hydrolysis. J. Biol. Chem. 271, 27983-27986 (1996).
5. Kowalczykowski, S.C., Dixon, D.A., Eggleston, A.K., Lauder, S.D. & Rehrauer, W.M. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58, 401-465 (1994).
6. Cox, M.M. The bacterial RecA protein as a motor protein. Annu. Rev. Microbiol. 57, 551-577 (2003).
7. Pellegrini, L. et al. Insights into DNA recombination from the structure of a Rad51-BRCA2 complex. Nature 420, 287-293 (2002).
8. Story, R.M., Weber, I.T. & Steitz, T.A. The structure of the E. coli recA protein monomer and polymer. Nature 355, 318-325 (1992).
9. Shin, D.S. et al. Full-length archaeal Rad51 structure and mutants: mechanisms for Rad51 assembly and control by BRCA2. EMBOJ, 22, 4566-4576 (2003).
10. Singleton, M.R., Sawaya, M.R., Ellenberger, T. & Wigley, D.B. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides. Cell 101, 589-600 (2000).
11. Niedenzu, T., Roleke, D., Bains, G., Scherzinger, E. & Saenger, W. Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010. J. Mol. Biol. 306, 479-487 2001.
12. Abrahams, J.P., Leslie, A.G., Lutter, R. & Walker, J.E. Structure at 2.8 Å resolution of F1-ATPase from bovine heart mitochondria. Nature 370, 621-628 (1994).
13. Caruthers, J.M. & McKay, D.B. Helicase structure and mechanism. Curr. Opin. Struct. Biol. 12, 123-133 (2002).
14. Aihara, H., Ito, Y., Kurumizaka, H., Yokoyama, S. & Shibata, T. The N-terminal domain of the human Rad51 protein binds DNA: structure and a DNA binding surface as revealed by NMR. J. Mol. Biol. 290, 495-504 (1999).
15. Yu, X., Jacobs, S.A., West, S.C., Ogawa, T. & Egelman, E.H. Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA. Proc. Natl. Acad. Sci. USA 98, 8419-8424 (2001).
16. Egelman, E.H. Does a stretched DNA structure dictate the helical geometry of RecA-like filaments? J. Mol. Biol. 309, 539-542 (2001).
17. VanLoock, M.S. et al. Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments. J. Mol. Biol. 333, 345-354 (2003).
18. VanLoock, M.S. et al. ATP-mediated conformational changes in the RecA filament. Structure 11, 187-196 (2003).
19. Kelley De Zutter, J., Forget, A.L., Logan, K.M. & Knight, K.L. Phe217 regulates the transfer of allosteric information across the subunit interface of the RecA protein filament. Structure 9, 47-55 (2001).
20. Lauder, S.D. & Kowalczykowski, S.C. Asymmetry in the recA protein-DNA filament. J. Biol. Chem. 266, 5450-5458 (1991).
21. Fortin, G.S. & Symington, L.S. Mutations in yeast Rad51 that partially bypass the requirement for Rad55 and Rad57 in DNA repair by increasing the stability of Rad51-DNA complexes. EMBO J. 21, 3160-3170 (2002).
22. Datta, S. et al. Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AIF(4): implications for decreased ATPase activity and molecular aggregation. Nucleic Acids Res. 28, 4964-4973 (2000).
23. Datta, S., Ganesh, N., Chandra, N.R., Muniyappa, K. & Vijayan, M. Structural studies on MtRecA-nucleotide complexes: insights into DNA and nucleotide binding and the structural signature of NTP recognition. Proteins 50, 474-485 (2003).
24. Yuan, Z.M. et al. Regulation of Rad51 function by c-Abl in response to DNA damage. J. Biol. Chem. 273, 3799-3802 (1998).
25. Chen, G. et al. Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl. J. Biol. Chem. 274, 12748-12752 (1999).
26. Menge, K.L. & Bryant, F.R. ATP-stimulated hydrolysis of GTP by RecA protein: kinetic consequences of cooperative RecA protein-ATP interactions. Biochemistry 27, 2635-2640 (1988).
27. Hingorani, M.M., Washington, M.T., Moore, K.C. & Patel, S.S. The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase. Proc. Natl. Acad. Sci. USA 94, 5012-5017 (1997).
28. Marians, K.J. Crawling and wiggling on DNA: structural insights to the mechanism of DNA unwinding by helicases. Structure Fold. Des. 8, R227-R235 (2000).
29. Zaitseva, E.M., Zaitsev, E.N. & Kowalczykowski, S.C. The DNA binding properties of Saccharomyces cerevisiae Rad51 protein. J. Biol. Chem. 274, 2907-2915 (1999).
30. Takahashi, M. & Norden, B. Structure of RecA-DNA complex and mechanism of DNA strand exchange reaction in homologous recombination. Adv. Biophys. 30, 1-35 (1994).
31. Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326 (1997).
32. Kissinger, C.R., Gehlhaar, D.K. & Fogel, D.B. Rapid automated molecular replacement by evolutionary search. Acta Crystallogr. D 55, 484-491 (1999).
33. Cowtan, K.D. & Zhang, K.Y. Density modification for macromolecular phase improvement. Prog. Biophys. Mol. Biol. 72, 245-270 (1999).
34. Brunger, A.T. et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905-921 (1998).
35. Collaborative Computational Project, Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D 50, 760-763 (1994).
36. Winn, M.D., Isupov, M.N. & Murshudov, G.N. Use of TLS parameters to model anisotropic displacements in macromolecular refinement. Acta Crystallogr. D 57, 122-133 (2001).
37. Carson, M. Ribbons. Methods Enzymol. 277, 493-505 (1997).
38. Mazin, A.V., Zaitseva, E., Sung, P. & Kowalczykowski, S.C. Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing. EMBO J. 19, 1148-1156 (2000)
39. Story, R.M. & Steitz, T.A. Structure of the recA protein-ADP complex. Nature 355, 374-376 (1992).