Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing

EMBO Journal

Volumn 19, Issue 5, 2000, Pages 1148-1156

  Mazin, Alexander V ^a   Zaitseva, Elena ^a   Sung, Patrick ^b   Kowalczykowski, Stephen C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

DNA strand exchange; Homologous recombination; Joint molecule formation

Indexed keywords

DOUBLE STRANDED DNA; NUCLEOPROTEIN; RAD51 PROTEIN; RECA PROTEIN; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA RECOMBINATION; DNA REPAIR; DNA STRAND BREAKAGE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION;

BACTERIA (MICROORGANISMS); EUKARYOTA; NEGIBACTERIA;

EID: 0034161571     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.5.1148     Document Type: Article

References (34)

1. Anderson, D.G. and Kowalczykowski, S.C. (1997) The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a χ-regulated manner. Cell, 90, 77-86.
2. Baumann, P. and West, S.C. (1999) Heteroduplex formation by human Rad51 protein: effects of DNA end-structure, hRP-A and hRad52. J. Mol. Biol., 291, 363-374.
3. Benson, F.E., Stasiak, A. and West, S.C. (1994) Purification and characterization of the human Rad51 protein, an analogue of E.coli RecA. EMBO J., 13, 5764-5771.
4. Benson, F.E., Baumann, P. and West, S.C. (1998) Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature, 391, 401-404.
5. Cao, L., Alani, E. and Kleckner, N. (1990) A pathway for generation and processing of double-strand breaks during meiotic recombination in S.cerevisiae. Cell, 61, 1089-1101.
6. Cox, M.M. and Lehman, I.R. (1981a) Directionality and polarity in recA protein-promoted branch migration. Proc. Natl Acad. Sci. USA, 78, 6018-6022.
7. Cox, M.M. and Lehman, I.R. (1981b) RecA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange. Proc. Natl Acad. Sci. USA, 78, 3433-3437.
8. Gasior, S.L., Wong, A.K., Kora, Y., Shinohara, A. and Bishop, D.K. (1998) Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes. Genes Dev., 12, 2208-2221.
9. Gupta, R.C., Golub, E.I., Wold, M.S. and Radding, C.M. (1998) Polarity of DNA strand exchange promoted by recombination proteins of the RecA family. Proc. Natl Acad. Sci. USA, 95, 9843-9848.
10. Haber, J.E. (1995) In vivo biochemistry: physical monitoring of recombination induced by site-specific endonucleases. BioEssays, 17, 609-620.
11. Haber, J.E. (1997) A super new twist on the initiation of meiotic recombination. Cell, 89, 163-166.
12. Kogoma, T. (1996) Recombination by replication. Cell, 85, 625-627.
13. Konforti, B.B. and Davis, R.W. (1990) The preference for a 3′ homologous end is intrinsic to RecA-promoted strand exchange. J. Biol. Chem., 265, 6916-6920.
14. Konforti, B.B. and Davis, R.W. (1991) DNA substrate requirements for stable joint molecule formation by the RecA and single-stranded DNA-binding proteins of Escherichia coli. J. Biol. Chem., 266, 10112-10121.
15. Konforti, B.B. and Davis, R.W. (1992) ATP hydrolysis and the displaced strand are two factors that determine the polarity of RecA-promoted DNA strand exchange. J. Mol. Biol., 227, 38-53.
16. LeBowitz, J. (1985) Biochemical mechanism of strand initiation in bacteriophage λ DNA replication. PhD thesis, Johns Hopkins University, Baltimore, MD.
17. Mazin, A.V. and Kowalczykowski, S.C. (1996) The specificity of the secondary DNA binding site of RecA protein defines its role in DNA strand exchange. Proc. Natl Acad. Sci. USA, 93, 10673-10678.
18. Mazin, A.V. and Kowalczykowski, S.C. (1998) The function of the secondary DNA-binding site of RecA protein during DNA strand exchange. EMBO J., 17, 1161-1168.
19. Namsaraev, E.A. and Berg, P. (1998) Branch migration during Rad51-promoted strand exchange proceeds in either direction. Proc. Natl Acad. Sci. USA, 95, 10477-10481.
20. New, J.H., Sugiyama, T., Zaitseva, E. and Kowalczykowski, S.C. (1998) Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature, 391, 407-410.
21. Ogawa, T., Yu, X., Shinohara, A. and Egelman, E.H. (1993) Similarity of the yeast RAD51 filament to the bacterial RecA filament. Science, 259, 1896-1899.
22. Petukhova, G., Stratton, S. and Sung, P. (1998) Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature, 393, 91-94.
23. Register, J.C., III and Griffith, J. (1985) The direction of RecA protein assembly onto single strand DNA is the same as the direction of strand assimilation during strand exchange. J. Biol. Chem., 260, 12308-12312.
24. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Shinohara, A. and Ogawa, T. (1998) Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature, 391, 404-407.
26. Stasiak, A. and Di Capua, E. (1982) The helicity of DNA in complexes with recA protein. Nature, 299, 185-186.
27. Sugiyama, T., Zaitseva, E.M. and Kowalczykowski, S.C. (1997) A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein. J. Biol. Chem., 272, 7940-7945.
28. Sun, H., Treco, D. and Szostak, J.W. (1991) Extensive 3′-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site. Cell, 64, 1155-1161.
29. Sung, P. (1997a) Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J. Biol. Chem., 272, 28194-28197.
30. Sung, P. (1997b) Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Genes Dev., 11, 1111-1121.
31. Sung, P. and Robberson, D.L. (1995) DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell, 82, 453-461.
32. Sung, P. and Stratton, S.A. (1996) Yeast Rad51 recombinase mediates polar DNA strand exchange in the absence of ATP hydrolysis. J. Biol. Chem., 271, 27983-27986.
33. Szostak, J.W., Orr-Weaver, T.L., Rothstein, R.J. and Stahl, F.W. (1983) The double-strand break repair model for recombination. Cell, 33, 25-35.
34. Tracy, R.B., Baumohl, J.K. and Kowalczykowski, S.C. (1997) The preference for GT-rich DNA by the yeast Rad51 protein defines a set of universal pairing sequences. Genes Dev., 11, 3423-3431.