Gene induction and apoptosis in human hepatocellular carcinoma cells SMMC-7721 exposed to 5-aza-2′-deoxycytidine

Chinese Medical Journal

Volumn 120, Issue 18, 2007, Pages 1626-1631

  Fan, Hong ^a   Zhao, Zhu Jiang ^a   Cheng, Yu Chao ^a   Shan, Yun Feng ^a   Lu, Zhu Hong ^a   Zhang, Jian Qiong ^a   Xie, Wei ^a  

^a SOUTHEAST UNIVERSITY   (China)

Author keywords

5 aza 2 deoxycytidine; CpG islands; DNA methylation; Hepatocellular carcinoma

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; CARRIER PROTEIN; CYTOKINE; DNA; DNA METHYLTRANSFERASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTONE DEACETYLASE 2; METHYL CPG BINDING PROTEIN 2; REGULATOR PROTEIN;

5' UNTRANSLATED REGION; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL CYCLE; CELL CYCLE ARREST; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CPG ISLAND; DEVELOPMENTAL STAGE; DNA METHYLATION; DOWN REGULATION; DRUG MECHANISM; DRUG TARGETING; FLOW CYTOMETRY; GENE ACTIVATION; GENE EXPRESSION; GENE FUNCTION; GENE INDUCTION; GENE SILENCING; HUMAN; HUMAN CELL; INHIBITION KINETICS; LIVER CELL CARCINOMA; MICROARRAY ANALYSIS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE DATABASE; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; UPREGULATION; WESTERN BLOTTING;

EID: 35349019363     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.1097/00029330-200709020-00015     Document Type: Article

References (30)

1. Baylin SB, Herman JG, Graff JR, Vertino PM, Issa JP. Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res 1998; 72: 141-196.
2. Bender CM, Pao MM, Jones PA. Inhibition of DNA methylation by 5-aza-2′-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res 1998; 58: 95-101.
3. Wu J, Issa JP, Herman J, Bassett DE, Nelkin BD, Baylin SB. Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells. Proc Natl Acad Sci U S A 1993; 90: 8891-8895.
4. Jones PL, Wolffe AP. Relationships between chromatin organization and DNA methylation in determining gene expression. Semin Cancer Biol 1999; 9: 339-347.
5. Tazi J, Bird A. Alternative chromatin structure at CpG islands. Cell 1990; 60: 909-920.
6. Lee S, Lee HJ, Kim JH, Lee HS, Jang JJ, Kang GH. Aberrant CpG island methylation along multistep hepatocarcinogenesis. Am J Pathol 2003; 163: 1371-1378.
7. Ueki T, Toyota M, Sohn T, Yeo CJ, Issa JP, Hruban RH, et al. Methylation of multiple genes in pancreatic adenocarcinoma. Cancer Res 2000; 60: 1835-1839.
8. Venkatasubbarao K, Ammanamanchi S, Brattain MG; Mimari D, Freeman JW. Reversion of transcriptional repression of Sp1 by 5-aza-2′-deoxycytidine restores TGF-beta type II receptor expression in the pancreatic cancer cell line MIA PaCa-2. Cancer Res 2001; 61: 6239-6247.
9. Xie GS, Hou AR, Li LY, Gao YN, Cheng SJ. Aberrant p16 promoter methylation in bronchial mucosae as a biomarker for the early detection of lung cancer. Chin Med J 2006; 119: 1469-1472.
10. Petti MC, Mandelli F, Zagonel V, De Gregoris C, Merola MC, Latagliata R, et al. Pilot study of 5-aza-2′-deoxycytidine (Decitabine) in the treatment of poor prognosis acute myelogenous leukemia patients: preliminary results. Leukemia 1993; 7 Suppl 1: 36-41.
11. Jones PA. Laird PW. Cancer epigenetics comes of age. Nat Genet 1999; 21: 163-167.
12. Schena M, Shalon D, Davis RW, Brown PO. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995; 270: 467-470.
13. Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, Wodicka L, et al. A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell 1998; 2: 65-73.
14. Der SD, Zhou A, Williams BR, Silverman RH. Identification of genes differentially regulated by interferon alpha, beta, or gamma using oligonucleotide arrays. Proc Natl Acad Sci USA 1998; 95: 15623-15628.
15. Gray NS, Wodicka L, Thunnissen AM, Norman TC, Kwon S, Espinoza FH, et al. Exploiting chemical libraries, structure, and genomies in the search for kinase inhibitors. Science 1998; 281: 533-538.
16. Marton MJ, DeRisi JL, Bennett HA, Iyer VR, Meyer MR, Roberts CJ, et al. Drug target validation and identification of secondary drug target effects using DNA microarrays. Nat Med 1998; 4: 1293-1301.
17. Liu LH, Xiao WH, Liu WW. Effect of 5-Aza-2′-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2. World J Gastroenterol 2001; 7: 131-135.
18. Baylin S, Bestor TH. Altered methylation patterns in cancer cell genomes: cause or consequence? Cancer Cell 2002; 1: 299-305.
19. Grotzer MA, Eggert A, Zuzak TJ, Janss AJ, Marwaha S, Wiewrodt BR, et al. Resistance to TRAIL-induced apoptosis in primitive neuroectodermal brain tumor cells correlates with a loss of caspase-8 expression. Oncogene 2000; 19: 4604-4610.
20. Karpf AR, Moore BC, Ririe TO, Jones DA. Activation of the p53 DNA damage response pathway after inhibition of DNA methyltransferase by 5-aza-2′-deoxycytidine. Mol Pharmacol 2001; 59: 751-757.
21. Suh SI, Pyun HY, Cho JW, Baek WY, Park JB, Kwon T, et al. 5-Aza-2′-deoxycytidine leads to downregulation of aberrant p16INK4A RNA transcripts and restores the functional retinoblastoma protein pathway in hepatocellular carcinoma cell lines. Cancer Lett 2000; 160: 81-88.
22. Liang G, Gonzales FA, Jones PA, Orntoft TF, Thykjaer T. Analysis of gene induction in human fibroblasts and bladder cancer cells exposed to the methylation inhibitor 5-aza-2′-deoxycytidine. Cancer Res 2002; 62: 961-966.
23. Missiaglia E, Donadelli M, Palmieri M, Crnogorac-Jurcevic T, Scarpa A, Lemoine NR. Growth delay of human pancreatic cancer cells by methylase inhibitor 5-aza-2′-deoxycytidine treatment is associated with activation of the interferon signalling pathway. Oncogene 2005; 24: 199-211.
24. Bender CM, Pao MM, Jones PA. Inhibition of DNA methylation by 5-aza-2′-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res 1998; 58: 95-101.
25. Karpf AR, Peterson PW, Rawlis JT, Dalley BK, Yang Q, Albertsen H, et al. Inhibition of DNA methyltransferase stimulates the expression of signal transducer and activator of transcription 1, 2, and 3 genes in colon tumor cells. Proc Natl Acad Sci U S A 1999; 96: 14007-14012.
26. Soengas MS, Capodieci P, Polsky D, Mora J, Esteller M, Opitz-Araya X, et al. Inactivation of the apoptosis effector Apaf-1 in malignant melanoma. Nature 2001; 409: 207-211.
27. Ueki T, Takeuchi T, Nishimatsu H, Kajiwara T, Moriyama N, Narita Y, et al. Silencing of the caspase-1 gene occurs in murine and human renal cancer cells and causes solid tumor growth in vivo. Int J Cancer 2001; 91: 673-679.
28. Fang JY, Yang L, Zhu HY, Chen YX, Lu J, Lu R, et al. 5-Aza-2′-deoxycitydine induces demethylation and upregulates transcription of p16INK4A gene in human gastric cancer cell lines. Chin Med J 2004; 117: 99-103.
29. Pataer A, Fang B, Yu R, Kagawa S, Hunt KK, McDonnell TJ, et al. Adenoviral Bak overexpression mediates caspase-dependent tumor killing. Cancer Res 2000; 60: 788-792.
30. Tong QS, Zheng LD, Wang L, Liu J, Qian W. BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells. BMC Cancer 2004; 4: 33.