The degree of macromolecular crowding in the cytoplasm and nucleoplasm of mammalian cells is conserved

FEBS Letters

Volumn 581, Issue 26, 2007, Pages 5094-5098

  Guigas, Gernot ^a   Kalla, Claudia ^a   Weiss, Matthias ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Anomalous diffusion; Cytoplasm; Fluorescence correlation spectroscopy; Macromolecular crowding; Nucleus; Viscoelastic

Indexed keywords

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL SIZE; CYTOPLASM; DIFFUSION; FLUORESCENCE CORRELATION SPECTROSCOPY; HUMAN; HUMAN CELL; MACROMOLECULE; MAMMAL CELL; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; VISCOELASTICITY;

ANIMALS; CELL LINE; CELL NUCLEUS; CRICETINAE; CYTOPLASM; HUMANS; MACROMOLECULAR SUBSTANCES; MICE; VISCOSITY;

MAMMALIA;

EID: 35348858197     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.09.054     Document Type: Article

References (30)

1. Ward K., Li W., Zimmer S., and Davis T. Viscoelastic properties of transformed cells: role in tumor cell progression and metastasis formation. Biorheology 28 (1991) 301-313
2. Thoumine O., and Ott A. Comparison of the mechanical properties of normal and transformed fibroblasts. Biorheology 34 (1997) 309-326
3. Lekka M., Laidler P., Gil D., Lekki J., Stachura Z., and Hrynkiewicz A. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy. Eur. Biophys. J. 28 (1999) 312-316
4. Darling E., Zauscher S., Block J., and Guilak F. A thin-layer model for viscoelastic, stress-relaxation testing of cells using atomic force microscopy: do cell properties reflect metastatic potential?. Biophys. J. 92 (2007) 1784-1791
5. Wyckoff J., Jones J., Condeelis J., and Segall J. A critical step in metastasis: in vivo analysis of intravasation at the primary tumor. Cancer Res. 60 (2000) 2504-2511
6. Ben-Ze'ev A. The cytoskeleton in cancer cells. Biochim. Biophys. Acta 780 (1985) 197-212
7. Guck J., Schinkinger S., Lincoln B., Wottawah F., Ebert S., Romeyke M., Lenz D., Erickson H., Ananthakrishnan R., Mitchell D., Kas J., Ulvick S., and Bilby C. Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophys. J. 88 (2005) 3689-3698
8. Rowat A.C., Foster L.J., Nielsen M.M., Weiss M., and Ipsen J.H. Characterization of the elastic properties of the nuclear envelope. J. R. Soc. Interface 2 (2005) 63-69
9. Vaziri A., and Mofrad M. Mechanics and deformation of the nucleus in micropipette aspiration experiment. J. Biomech. 40 (2007) 2053-2062
10. Nickerson J. Experimental observations of a nuclear matrix. J. Cell Sci. 114 (2001) 463-474
11. Guigas G., Kalla C., and Weiss M. Probing the nanoscale viscoelasticity of intracellular fluids in living cells. Biophys. J. 93 (2007) 316-323
12. Ellis R., and Minton A. Cell biology: join the crowd. Nature 425 (2003) 27-28
13. Minton A. How can biochemical reactions within cells differ from those in test tubes?. J. Cell Sci. 119 (2006) 2863-2869
14. Weiss M., Elsner M., Kartberg F., and Nilsson T. Anomalous subdiffusion is a measure for cytoplasmic crowding in living cells. Biophys. J. 87 (2004) 3518-3524
15. Banks D., and Fradin C. Anomalous diffusion of proteins due to molecular crowding. Biophys. J. 89 (2005) 2960-2971
16. Kozer N., and Schreiber G. Effect of crowding on protein-protein association rates: fundamental differences between low and high mass crowding agents. J. Mol. Biol. 336 (2004) 763-774
17. Elson E. Fluorescence correlation spectroscopy measures molecular transport in cells. Traffic 2 (2001) 789-796
18. Weiss M., and Nilsson T. In a mirror dimly: tracing the movements of molecules in living cells. Trend Cell Biol. 14 (2004) 267-273
19. Mason T., and Weitz D. Optical measurements of frequency-dependent linear viscoelastic moduli of complex fluids. Phys. Rev. Lett. 74 (1995) 1250-1253
20. Saxton M. Lateral diffusion in an archipelago. Single-particle diffusion. Biophys. J. 64 (1993) 1766-1780
21. Saxton M. Lateral diffusion in an archipelago. Dependence on tracer size. Biophys. J. 64 (1993) 1053-1062
22. Santamaria F., Wils S., De Schutter E., and Augustine G. Anomalous diffusion in Purkinje cell dendrites caused by spines. Neuron 52 (2006) 635-648
23. Saxton M. A biological interpretation of transient anomalous subdiffusion. I. Qualitative model. Biophys. J. 92 (2007) 1178-1191
24. Tseng Y., Kole T., and Wirtz D. Micromechanical mapping of live cells by multiple-particle-tracking microrheology. Biophys. J. 83 (2002) 3162-3176
25. Tseng Y., Lee J., Kole T., Jiang I., and Wirtz D. Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J. Cell Sci. 117 (2004) 2159-2167
26. Tharmann R., Claessens M., and Bausch A. Viscoelasticity of isotropically cross-linked actin networks. Phys. Rev. Lett. 98 (2007) 21
27. Gardel M., Valentine M., Crocker J., Bausch A., and Weitz D. Microrheology of entangled F-actin solutions. Phys. Rev. Lett. 91 (2003) 7
28. Elsner M., Hashimoto H., Simpson J.C., Cassel D., Nilsson T., and Weiss M. Spatiotemporal dynamics of the COPI vesicle machinery. Embo Rep. 4 (2003) 1000-1005
29. Luby-Phelps K. Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area. Int. Rev. Cytol. 192 (2000) 189-221
30. Verkman A. Solute and macromolecule diffusion in cellular aqueous compartments. Trend Biochem. Sci. 27 (2002) 27-33