Rapid identification of pathogenic yeast isolates by real-time PCR and two-dimensional melting-point analysis

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 26, Issue 11, 2007, Pages 813-818

  Bergman, A ^a   Fernandez, V ^b   Holmstrom K O ^c   Claesson, B E B ^a   Enroth, H ^a  

^a Kärnsjukhuset ^*  (Sweden)

^b SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^c UNIVERSITY OF SKÖVDE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; PRIMER DNA; RIBOSOME DNA;

ANALYTIC METHOD; ARTICLE; CANDIDA; CRYPTOCOCCUS NEOFORMANS; FUNGAL STRAIN; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; MELTING POINT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

DNA PRIMERS; DNA, FUNGAL; DNA, RIBOSOMAL SPACER; HUMANS; MYCOSES; POLYMERASE CHAIN REACTION; TRANSITION TEMPERATURE; YEASTS;

EID: 35348856317     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-007-0369-2     Document Type: Article

References (24)

1. Gharizadeh B, Norberg E, Loeffler J, Jalal S, Tollemar J, Einsele H, Klingspor L, Nyren P (2004) Identification of medically important fungi by the Pyrosequencing technology. Mycoses 47:29-33
2. Leaw SN, Hsien CC, Hsiao FS, Barton R, Bouchara J-P, Tsung CC (2006) Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J Clin Microbiol 44:693-699
3. Trama JP, Mordechai E, Adelson ME (2005) Detection and identification of Candida species associated with Candida vaginitis by real-time PCR and pyrosequencing. Mol Cell Probes 19:145-152
4. Lau A, Chen S, Sorell T, Carter D, Malik R, Martin P, Halliday C (2007) Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 45:380-385
5. Linton CJ, Borman AM, Cheung G, Holmes AD, Szekely A, Palmer MD, Bridge PD, Campbell CK, Johnson EM (2007) Molecular identification of unusual pathogenic yeast isolates by large ribosomal subunit gene sequencing: 2 years of experience at the United Kingdom Mycology Reference Laboratory. J Clin Microbiol 45:1152-1158
6. Selvarangan R, Limaye AP, Cookson BT (2002) Rapid identification and differentiation of Candida albicans and Candida dublinensis by capillary-based amplification and fluorescent probe hybridization. J Clin Microbiol 40:4308-4312
7. Innings Å, Ullberg M, Johansson A, Rubin CJ, Noreus N, Isaksson M, Herrmann B (2007) Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood. J Clin Microbiol 45:874-880
8. Hendolin P, Paulin L, Koukkila-Kähkölä P, Anttila V, Malmberg H, Richardsson M, Ylikoski J (2002) Panfungal PCR and multiplex liquid hybridization for detection of fungi in tissue specimens. J Clin Microbiol 38:4186-4192
9. Luo G, Mitchell TG (2002) Rapid identification of pathogenic fungi directly from cultures using multiplex PCR. J Clin Microbiol 40:2860-2865
10. Klingspor L, Jalal S (2006) Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR. Clin Microbiol Infect 12:745-753
11. Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, Einsele H (2000) Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J Clin Microbiol 38:586-590
12. Maaroufi Y, De Bruyne J-M, Duchateau V, Georgala A, Crokaert F (2004) Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay. J Molecul Diagnost 6:108-114
13. Maaroufi Y, Heymans C, De Bruyne J-M, Duchateau V, Rodriguez-Villalobos H, Aoun M, Crokaert F (2003) Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J Clin Microbiol 41:3293-3298
14. Van Burik J, Myerson D, Schreckhise R, Bowden R (1998) Panfungal PCR assay for detection of fungal infection in human blood specimens. J Clin Microbiol 36:1169-1175
15. Schabereiter-Gurtner C, Selitsch B, Rotter ML, Hirschl AM, Willinger B (2007) Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol 45:906-914
16. Hsu MC, Chen KW, Lo H J, Liao MH, Lin YH, Li SY (2003) Species identification of medically important fungi by use of real-time LightCycler PCR. J Clin Microbiol 52:1071-1076
17. Huang A, Li JW, Shen ZQ, Wang XW, Jin M (2006) High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray. J Clin Microbiol 44:3299-3305
18. Mirhendi H, Makimura K, Khoramizadeh M, Yamaguchi H (2006) A one-enzyme PCR-RFLP assay for identification of six medically important Candida species. Jpn J Med Mycol 47:225-229
19. Chen S, Halliday C, Meyer W (2002) A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Med Mycol 40:333-357
20. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351-1371
21. Casey GD, Dobson ADW (2004) Potential of using real-time PCR-based detection of spoilage yeast in fruit juice: a preliminary study. Int J Food Microbiol 91:327-335
22. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky J, White TJ. PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315-322
23. Søgaard M, Stender H, Schønheyder H (2004) Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes. J Clin Microbiol 43:1947-1949
24. Skow Á, Mangold KA, Tajuddin M, Huntington A, Fritz B, Thomson RB Jr, Kaul KL (2005) Species-level identification of staphylococcal isolates by real-time PCR and melt curve analysis. J Clin Microbiol 43:2876-2880