Species-level identification of staphylococcal isolates by real-time PCR and melt curve analysis

Journal of Clinical Microbiology

Volumn 43, Issue 6, 2005, Pages 2876-2880

  Skow, Áine ^a   Mangold, Kathy A ^a   Tajuddin, Mohammed ^a   Huntington, Anne ^a   Fritz, Brett ^a   Thomson Jr , Richard B ^a   Kaul, Karen L ^a,b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b Evanston Northwestern Healthcare ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COAGULASE; PROTEIN A; RNA 16S;

AGGLUTINATION TEST; AMPLICON; ARTICLE; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CLINICAL LABORATORY; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RIBOTYPING; RNA GENE; SPECIES IDENTIFICATION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HAEMOLYTICUS; STAPHYLOCOCCUS HOMINIS; STAPHYLOCOCCUS LUGDUNENSIS; STAPHYLOCOCCUS SIMULANS;

BACTERIAL TYPING TECHNIQUES; BLOOD; DNA PROBES; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECIES SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS; TRANSITION TEMPERATURE;

EID: 20444472365     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.6.2876-2880.2005     Document Type: Article

References (24)

1. Bannerman, T. L. 2003. Staphylococcus, Micrococcus, and other catalase-positive cocci that grow aerobically, p. 391. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. American Society for Microbiology, Washington, D.C.
2. Bingen, E. H., E. Denamur, and J. Elion. 1994. Use of ribotyping in epidemiological surveillance of nosocomial outbreaks. Clin. Microbiol. Rev. 7:311-327.
3. Chambers, H. F. 1997. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10:781-791.
4. Christensen, G. D., J. T. Parisi, A. L. Bisno, W. A. Simpson, and E. H. Beachey. 1983. Characterization of clinically significant strains of coagulase-negative staphylococci. J. Clin. Microbiol. 18:258-269.
5. Christensen, G. D., W. A. Simpson, A. L. Bisno, and E. H. Beachey. 1982. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect. Immun. 37:318-326.
6. Cormican, M. G., and R. N. Jones. 1996. Emerging resistance to antimicrobial agents in gram-positive bacteria. Enterococci, staphylococci and non-pneumococcal streptococci. Drugs 51(Suppl. 1):6-12.
7. Edwards, K. J., M. E. Kaufmann, and N. A. Saunders. 2001. Rapid and accurate identification of coagulase-negative staphylococci by real-time PCR. J. Clin. Microbiol. 39:3047-3051.
8. Eng, R. H., C. Wang, A. Person, T. E. Kiehn, and D. Armstrong. 1982. Species identification of coagulase-negative staphylococcal isolates from blood cultures. J. Clin. Microbiol. 15:439-442.
9. Gahrn-Hansen, B. 1987. Coagulase-negative staphylococci and micrococci in clinical microbiology. Dan. Med. Bull. 34:96-114.
10. Gill, V. J., S. T. Selepak, and E. C. Williams. 1983. Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimens. J. Clin. Microbiol. 18:1314-1319.
11. Hussain, Z., L. Stoakes, V. Massey, D. Diagre, V. Fitzgerald, S. El Sayed, and R. Lannigan. 2000. Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci. J. Clin. Microbiol. 38:752-754.
12. Kim, S. D., L. C. McDonald, W. R. Jarvis, S. K. McAllister, R. Jerris, L. A. Carson, and J. M. Miller. 2000. Determining the significance of coagulase-negative staphylococci isolated from blood cultures at a community hospital: a role for species and strain identification. Infect. Control Hosp. Epidemiol. 21:213-217.
13. Kloos, W. E., and T. L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7:117-140.
14. Lowy, F. D. 1998. Staphylococcus aureus infections. N. Engl. J. Med. 339:520-532.
15. Lowy, F. D., and S. M. Hammer. 1983. Staphylococcus epidermidis infections. Ann. Intern. Med. 99:834-839.
16. Marshall, S. A., W. W. Wilke, M. A. Pfaller, and R. N. Jones. 1998. Staphylococcus aureus and coagulase-negative staphylococci from blood stream infections: frequency of occurrence, antimicrobial susceptibility, and molecular (mecA) characterization of oxacillin resistance in the SCOPE program. Diagn. Microbiol. Infect. Dis. 30:205-214.
17. Martin, M. A., M. A. Pfaller, and R. P. Wenzel. 1989. Coagulase-negative staphylococcal bacteremia. Mortality and hospital stay. Ann. Intern. Med. 110:9-16.
18. Panlilio, A. L., D. H. Culver, R. P. Gaynes, S. Banerjee, T. S. Henderson, J. S. Toison, and W. J. Martone. 1992. Methicillin-resistant Staphylococcus aureus in U.S. hospitals, 1975-1991. Infect. Control Hosp. Epidemiol. 13:582-586.
19. Pfaller, M. A., and L. A. Herwaldt. 1988. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clin. Microbiol. Rev. 1:281-299.
20. Reischl, U., M. Pulz, W. Ehret, and H. Wolf. 1994. PCR-based detection of mycobacteria in sputum samples using a simple and reliable DNA extraction protocol. BioTechniques 17:844-845.
21. Sewell, C. M. 1984. Coagulase-negative staphylococci and the clinical microbiology laboratory. Eur. J. Clin. Microbiol. 3:94-95.
22. Sewell, C. M., J. E. Clarridge, E. J. Young, and R. K. Guthrie. 1982. Clinical significance of coagulase-negative staphylococci. J. Clin. Microbiol. 16:236-239.
23. Smith, D. J., R. L. Kaplan, W. Landau, and G. M. Trenholme. 1982. Speciation and antibiotic susceptibility patterns of coagulase-negative staphylococci. Eur. J. Clin. Microbiol. 1:228-232.
24. Weinstein, M. P., S. Mirrett, L. Van Pelt, M. McKinnon, B. L. Zimmer, W. Kloos, and L. B. Reller. 1998. Clinical importance of identifying coagulase-negative staphylococci isolated from blood cultures: evaluation of MicroScan and dried overnight gram-positive panels versus a conventional reference method. J. Clin. Microbiol. 36:2089-2092.