The slit receptor EVA-1 coactivates a SAX-3/robo-mediated guidance signal in C. elegans

Science

Volumn 317, Issue 5846, 2007, Pages 1934-1938

  Fujisawa, Kazuko ^a   Wrana, Jeffrey L ^a,b   Culotti, Joseph G ^a,b  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; GALACTOSE; GALAPTIN; PROTEIN SAX3; ROUNDABOUT RECEPTOR; SLIT PROTEIN; UNCLASSIFIED DRUG; VEROTOXIN 1;

CANCER; MUTATION;

ARTICLE; BINDING KINETICS; BINDING SITE; CAENORHABDITIS ELEGANS; CELL MIGRATION; COLORECTAL CANCER; GENE FUNCTION; GENE MUTATION; MOLECULAR BIOLOGY; NERVE CELL; NERVE FIBER; NERVE FIBER DEGENERATION; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; ANIMALS, GENETICALLY MODIFIED; AXONS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CARRIER PROTEINS; CELL LINE; CELL MOVEMENT; CLONING, MOLECULAR; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; NERVE TISSUE PROTEINS; NERVOUS SYSTEM; NEURONS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, IMMUNOLOGIC; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION;

CAENORHABDITIS ELEGANS;

EID: 34948841474     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1144874     Document Type: Article

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18. This work was supported by grants from the Canadian Institutes of Health Research and NIH (grant NS41397) (J.G.C.) and was funded by Genome Canada through the Ontario Genomics Institute (J.L.W.). We thank C. Bargmann (Rockefeller Univ.) for myo-3p::SLT-1(kyIs218) and sax-3 alleles; M. Letarte (Univ. of Toronto) for the TGFbetaIIR::3xFLAG construct; the C. elegans genome consortium and A. Coulson for cosmids; Y. Kohara (National Institute of Genetics, Mishima, Japan) for cDNAs; A. Fire (Carnegie Institute of Washington, DC) for GFP vectors; S. Mitani (Tokyo Women's Medical Univ.) for eva-1 (tm0974); L. MacNeil for helpful discussion regarding binding experiments and for UNC-40::3xFLAG; J. Plummer, R. Ikegami, and N. Levy-Strumpf for their critical reading of the manuscript; L. Brown for confocal microscopy; and W. Hung and H. Zheng for help with other experiments. Some strains not derived in our laboratory were obtained from the Caenorhabditis Genetics Center (courtesy of T. Stiernagle), which is funded by the NIH National Center for Research Resources. For human and mouse putative homologs (HuC21orf63 and MmC21orf63), the GenBank accession numbers are AF358258 and AF358257, respectively, and for C. elegans EVA-1 protein, the accession number is NP_493590.2.