The nitrate reductase gene-switch: A system for regulated expression in transformed cells of Dunaliella salina

Gene

Volumn 403, Issue 1-2, 2007, Pages 132-142

  Li, Jie ^a   Xue, Lexun ^a   Yan, Hongxia ^a   Wang, Lili ^a   Liu, Lingling ^a   Lu, Yumin ^a   Xie, Hua ^a,b  

^a THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

^b SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

bar gene; Dunaliella salina; Expression; Nitrate reductase; Promoter

Indexed keywords

AMMONIA; MESSENGER RNA; NITRATE REDUCTASE;

ARTICLE; CELL TRANSFORMATION; CONTROLLED STUDY; EUKARYOTE; FLUORESCENCE; GENE EXPRESSION; GENE SWITCHING; GENETIC TRANSCRIPTION; GENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; TRANSGENIC ORGANISM;

ALGAE, GREEN; AMINOBUTYRIC ACIDS; BASE SEQUENCE; CELL LINE, TRANSFORMED; CLONING, MOLECULAR; CODON, INITIATOR; CODON, TERMINATOR; DNA PRIMERS; DNA, ALGAL; ENZYME INHIBITORS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENOME; KINETICS; MOLECULAR SEQUENCE DATA; NITRATE REDUCTASES; NITRATES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PHYLOGENY; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); QUATERNARY AMMONIUM COMPOUNDS; RESTRICTION MAPPING; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SPECTROPHOTOMETRY, ULTRAVIOLET;

DUNALIELLA SALINA;

EID: 34748832242     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2007.08.001     Document Type: Article

References (30)

1. Ben-Amotz A., and Avron M. The biotechnology of cultivating the halotolerant alga Dunaliella. Trends Biotech. 8 (1990) 121-126
2. Cannons C.A., and Shiflett S.D. Transcriptional regulation of the nitrate reductase gene in Chlorella vulgaris: identification of regulatory elements controlling expression. Curr. Genet. 40 (2001) 128-135
3. Dawson H.N., Pendleton L.C., Solomonson L.P., and Cannons A.C. Cloning and characterization of the nitrate reductase-encoding gene from Chlorella vulgaris: structure and identification of transcription start points and initiator sequences. Gene 171 (1996) 39-145
4. Fernandez E., and Cardenas J. Regulation of the nitrate-reducing system enzymes in wild-type and mutant strains of Chlamydomonas reinhardtii. Mol. Gen. Genet. 186 (1982) 164-169
5. Fu X.D., et al. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res. 9 (2000) 11-19
6. Geng D.G., Wang Y.Q., Li W.B., and Sun Y.R. Transient expression of gus gene in Dunaliella salina. High Tech. Lett. 2 (2002) 35-40
7. Geng D.G., Wang Y.Q., Wang P., Li W.B., and Sun Y.R. Stable expression of hepatitis B surface antigen gene in Dunaliella salina (Chlorophyta). J. Appl. Phycol. 15 (2003) 451-456
8. He Q.H., et al. Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina. J. Appl. Phycol. 16 (2004) 395-400
9. Jiang G.Z., Lu Y.M., Niu X.L., and Xue L.X. The actin gene promoter-driven bar as a dominant selectable marker for nuclear transformation of Dunaliella salina. Acta Genet. Sin. 32 (2005) 424-433
10. Koblenz B., and Lechtreck K.F. The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii. Eukaryot. Cell 4 (2005) 1959-1962
11. Liu X., and Gorovsky M.A. Mapping the 5′ and 3′ ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucl. Acid Res. 21 (1993) 4954-4960
12. Li Z.X., Sun Y., Liu Z.X., Song R.T., and Xu Z.K. Transient expression of foreign ble gene in Duanliella salina. Acta Biol. Exp. Sin. 38 (2005) 411-416
13. Li J., Qu D.J., Liu L.L., Feng S.Y., and Xue L.X. Comparison of stable expressions of foreign genes driven by different promoters in transgenic Dunaliella salina. China Biotech. 27 3 (2007) 47-53
14. Lopez-Ruiz A., Verbelen J.P., Roldan J.M., and Diez J. Nitrate reductase of green algae is located in the pyrenoid. Plant Physiol. 79 (1985) 1006-1010
15. Loppes R., and Radoux M. Two short regions of the promoter are essential for activation and repression of the nitrate reductase gene in Chlamydomonas reinhardtii. Mol. Genet. Genomics 268 (2002) 42-48
16. Loppes R., Radoux M., Ohresser M.C., and Matagne R.F. Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nia1 promoter. Plant Mol. Biol. 41 (1999) 701-711
17. Lu Y.M., Jiang G.Z., Niu X.L., Hou G.Q., Zhang G.Q., and Xue L.X. Cloning and functional analysis of promoter of two carbonic anhydrase genes from Dunaliella salina. Acta Genet. Sin. 31 10 (2004) 1157-1166
18. Mullick A., Xu Y., and Warren R. The cumate gene-switch: a system for regulated expression in mammalian cells. BMC Biotechnol. 6 (2006) 43-61
19. Muro-Pastor M.I., Gonzalez R., Strauss J., Narendja F., and Scazzocchio C. The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter. EMBO J. 18 (1999) 1584-1597
20. Overbergh L., Giulietti A., Valckx D., Decallonne B., Bouillon R., and Mathieu C. The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. J. Biomol. Tech. 14 (2003) 33-43
21. Poulsen N., and Kroger N.A. A new molecular tool for transgenic diatoms: control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette. FEBS J. 272 (2005) 3413-3423
22. Price-Schiavi S.A., Perez A., Barco R., and Carraway K.L. Cloning and characterization of the 5′ flanking region of the sialomucin complex/rat Muc4 gene: promoter activity in cultured cells. Biochem J. 349 (2000) 641-649
23. Smith C.L., and Hager G.L. Transcriptional regulation of mammalian genes in vivo. A tale of two templates. J. Biol. Chem. 272 44 (1997) 27493-27496
24. Somers D.A., and Makarevitch I. Transgene integration in plants: poking or patching holes in promiscuous genomes?. Curr. Opin. Biotechnol. 15 2 (2004) 126-131
25. Warning H.O., and Hachtel W. Functional analysis of a nitrite reductase promoter from birch in transgenic tobacco. Plant Sci. 155 (2000) 141-151
26. Wang P., Sun Y.R., Li X., Zhang L.M., Li W.B., and Wang Y.Q. Rapid isolation and functional analysis of promoter sequences of the nitrate reductase gene from Chlorella ellipsoidea. J. Appl. Phycol. 16 (2004) 11-16
27. Warrens A.N., Jones M.D., and Lechler R.I. Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest. Gene 186 (1997) 29-35
28. Wojciech P., Pawlowski, and Somers D.A. Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 12106-12110
29. Xie H., Xu P.Y., Jia Y.L., Li J., and Xue L.X. Cloning and heterologous expression of nitrate reductase genes from Dunaliella salina. J. Appl. Phycol. 19 (2007) 497-504
30. Zhang G.X., Xue P.R., Zheng H.M., Wang J.M., and Xue L.X. The resistance of Dunaliella salina to the herbicide phosphinothricin. Life Sci. Res. 6 (2002) 92-94