Engineering, expression, purification, and production of recombinant thermolysin

Biotechnology Annual Review

Volumn 13, Issue , 2007, Pages 43-64

  Inouye, Kuniyo ^a   Kusano, Masayuki ^a   Hashida, Yasuhiko ^a   Minoda, Masashi ^b   Yasukawa, Kiyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Daiwa Kasei KK   (Japan)

Author keywords

affinity chromatography; Bacillus stearothermophilus; Bacillus subtilis; Bacillus thermoproteolyticus; engineering; enzyme; Escherichia coli; expression; glycyl d phenylalanine; hydrophobic interaction chromatography; inclusion body; metalloproteinase; NprM; production; propeptide; protein engineering; purification; recombinant protein; thermolysin; TLP ste.

Indexed keywords

ASPARTAME; ESTER DERIVATIVE; METALLOPROTEINASE; RECOMBINANT ENZYME; SWEETENING AGENT; THERMOLYSIN;

BACILLUS; BACTERIUM CULTURE; CATALYSIS; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; GENETIC ENGINEERING; INDUSTRY; NONHUMAN; PROTEIN EXPRESSION; REVIEW; THERMOSTABILITY;

BACILLUS; ESCHERICHIA COLI; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; THERMOLYSIN;

BACILLUS SUBTILIS; BACILLUS THERMOPROTEOLYTICUS; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 34548580171     PISSN: 13872656     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S1387-2656(07)13003-9     Document Type: Review

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