Structural and genetic analysis of the folding and function of T4 lysozyme

FASEB Journal

Volumn 10, Issue 1, 1996, Pages 35-41

  Matthews, Brian W ^a  

^a UNIVERSITY OF OREGON   (United States)

Author keywords

alanine; hydrophobicity cavities; protein stability

Indexed keywords

LYSOZYME; VIRUS ENZYME;

BACTERIOPHAGE T4; CATALYSIS; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME STABILITY; HYDROPHOBICITY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; REVIEW; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

EID: 0030045089     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.10.1.8566545     Document Type: Review

References (29)

1. Reidhaar-Olson, J. F., and Sauer, R. T. (1988) Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences. Science 241, 53-57
2. Matthews, B. W. (1987) Genetic and structural analysis of the protein stability problem. Biochemistry 26, 6885-6888
3. Kamtekar, S., Schiffer, J. M., Ziong, H., Babik, J. M., and Hecht, M. H. (1993) Protein design by binary patterning of polar and nonpolar amino acids. Science 262, 1680-1685
4. Zhang, X-J., Baase, W. A., and Matthews, B. W. (1991) Toward a simplification of the protein folding problem: A stabilizing polyalanine α-helix engineered in T4 lysozyme. Biochemistry 30, 2012-2017
5. Heinz, D. W., Baase, W. A., and Matthews, B. W. (1992) Folding and function of a T4 lysozyme containing 10 consecutive alanines illustrate the redundancy of information in an amino acid sequence. Proc. Natl. Acad. Sci. USA 89, 3751-3755
6. Zhang, X-J., Baase, W. A., and Matthews, B. W. (1992) Multiple alanine replacements within α-helix 126-134 of T4 lysozyme have independent, additive effects on both structure and stability. Protein Sci. 1, 761-776
7. Blaber, M., Baase, W. A., Gassner, N., and Matthews, B. W. (1995) Alanine scanning mutagenesis of the α-helix 115-123 of phage T4 lysozyme: Effects on structure, stability and the binding of solvent. J. Mol. Biol. 246, 317-330
8. Chothia, C. (1974) Hydrophobic bonding and accessible surface area in proteins. Nature (London) 248, 338-339
9. Richards, F. M. (1977) Areas, volumes, packing and protein structure. Annu. Rev. Biophys. Bioeng. 6, 151-176
10. Kellis, J. T., Jr., Nyberg, K., and Fersht, A. R. (1989) Energetics of complementary side-chain packing in a protein hydrophobic core. Biochemistry 28, 4914-4922
11. Shortle, D., Stites, W. E., and Meeker, A. K. (1990) Contributions of the large hydrophobic amino acids to the stability of staphylococcal nuclease. Biochemistry 29, 8033-8041
12. Sandberg, W. S., and Terwilliger, T. C. (1991) Energetics of repacking a protein interior. Proc. Natl Acad. Sci. USA 88, 1706-1710
13. Pace, C. N. (1992) Contribution of the Hydrophobic effect to globular protein stability. J. Mol. Biol. 226, 29-35
14. Eriksson, A. E., Baase, W. A., Zhang, X-J., Heinz, D. W., Blaber, M., Baldwin, E. P., and Matthews, B. W. (1992) The response of a protein structure to cavity-creating mutations and its relationship to the Hydrophobic effect. Science 255, 178-183
15. Xu, J., Baase, W., and Matthews, B. W. (1995) The hydrophobic effect, cavities and an internal water molecule m T4 lysozyme. Am. Cryst. Assn. Abstr. 23, 154
16. Eriksson, A. E., Baase, W. A., Wozniak, J. A., and Matthews, B. W. (1992) A cavity-containing mutant of T4 lysozyme is stabilized by buried benzene. Nature (London) 355, 371-373
17. Morton, A., Baase, W. A., and Matthews, B. W. (1995) Energetic origins of specificity of ligand binding in an interior nonpolar cavity of T4 lysozyme. Biochemistry 34, 8564-8575
18. Morton, A., and Matthews, B. W. (1995) Specificity of ligand binding in a buried nonpolar cavity of T4 lysozyme: Linkage of dynamics and structural plasticity. Biochemistry 34, 8576-8588
19. Matthews, B. W. (1993) Structural and genetic analysis of protein stability. Annu. Rev. Biochem. 62, 139-160
20. Pauling, L., Campbell, D. H., and Pressman, D. (1943) The nature of the forces between antigen and antibody and of the precipitation reaction. Physiol. Rev. 23, 203-219
21. Fersht, A. R., Shi, J-P., Knill-Jones, J., Lowe, D. M., Wilkinson, A. J., Blow. D. M., Brick, P., Carter, P., Waye, M. M. Y., and Winter, G. (1985) Hydrogen bonding and biological specificity analysed by protein engineering. Nature (London) 314, 235-238
22. Warshel, A. (1987) What about protein polarity? Nature (London) 330, 15-16
23. Shoichet, B. K., Baase, W. A., Kuroki, R., and Matthews, B. W. (1995) A relationship between protein stability and protein function. Proc. Natl. Acad. Sci. USA 92, 452-456
24. Kuroki, R., Weaver, L. H., and Matthews, B. W. (1993) A covalent enzyme-substrate intermediate with saccharide distortion in a mutant T4 lysozyme. Science 262, 2030-2033
25. Kuroki, R., Weaver, L. H., and Matthews, B. W. (1995) Structure-based design of a lysozyme with altered catalytic activity. Nature Struct. Biol. 2, 1007-1011
26. Anand, N. N., Stephen, E. R., and Narang, S. A. (1988) Mutation of active site residues in synthetic T4-lysozyme gene and their effect on lytic activity. Biochem. Biophys. Res. Commun. 153, 862-068
27. 20 in catalysis by bacteriophage T4 lysozyme. Biochemistry 30, 9457-9463
28. Rennell, D., Bouvier, S. E., Hardy, L. W., and Poteete, A. R. (1991) Systematic mulation of bacteriophage T4 lysozyme. J. Mol. Biol. 222, 67-87
29. Dahlquist, F. W., Borders, C. L., Jacobson, G., and Raftery, M. A. (1969) The stereospecificity of human, hen, and papaya lysozymes. Biochemistry 8, 694-700