-
1
-
-
0017805504
-
Active centers of Streptomyces griseus protease 1, Streptomyces griseus protease 3, and α-chymotrypsin: Enzyme-substrate interactions
-
Bauer, C.-A. (1978). Active centers of Streptomyces griseus protease 1, Streptomyces griseus protease 3, and α-chymotrypsin: enzyme-substrate interactions. Biochemistry, 17, 375-380.
-
(1978)
Biochemistry
, vol.17
, pp. 375-380
-
-
Bauer, C.-A.1
-
2
-
-
0026347727
-
Mutational remodeling of enzyme specificity
-
Bone, R. & Agard, D. A. (1991). Mutational remodeling of enzyme specificity Methods Enzymol. 202, 643-671.
-
(1991)
Methods Enzymol.
, vol.202
, pp. 643-671
-
-
Bone, R.1
Agard, D.A.2
-
3
-
-
0024973407
-
Structural plasticity broadens the specificity of an engineered protease
-
Bone, R., Silen, J. L. & Agard, D. A. (1989). Structural plasticity broadens the specificity of an engineered protease. Nature, 339, 191-195.
-
(1989)
Nature
, vol.339
, pp. 191-195
-
-
Bone, R.1
Silen, J.L.2
Agard, D.A.3
-
4
-
-
0018287541
-
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
-
Chang, S. & Cohen, S. N. (1979). High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol. Gen. Genet. 168, 111-115.
-
(1979)
Mol. Gen. Genet.
, vol.168
, pp. 111-115
-
-
Chang, S.1
Cohen, S.N.2
-
5
-
-
0027205210
-
Tuning the activity of an enzyme for unusual environments: Sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
-
Chen, K. & Arnold, F. H. (1993). Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide. Proc. Natl Acad. Sci. USA, 90, 5618-5622.
-
(1993)
Proc. Natl Acad. Sci. USA
, vol.90
, pp. 5618-5622
-
-
Chen, K.1
Arnold, F.H.2
-
6
-
-
0021827776
-
Redesigning trypsin: Alteration of substrate specificity
-
Craik, C. S., Largman, C., Fletcher, T., Roczniak, S., Barr, P. J., Fletterick, R. J. & Rutter, W. J. (1985). Redesigning trypsin: alteration of substrate specificity Science, 228, 291-297.
-
(1985)
Science
, vol.228
, pp. 291-297
-
-
Craik, C.S.1
Largman, C.2
Fletcher, T.3
Roczniak, S.4
Barr, P.J.5
Fletterick, R.J.6
Rutter, W.J.7
-
7
-
-
0016700792
-
Tertiary structural differences between microbial serine proteases and pancreatic serine proteases
-
Delbaere, L. T. J., Hutcheon, W. L. B., James, M. N. G. & Thiessen, W. E. (1975). Tertiary structural differences between microbial serine proteases and pancreatic serine proteases. Nature, 257, 758-763.
-
(1975)
Nature
, vol.257
, pp. 758-763
-
-
Delbaere, L.T.J.1
Hutcheon, W.L.B.2
James, M.N.G.3
Thiessen, W.E.4
-
8
-
-
0022445901
-
Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering
-
Estell, D. A., Graycar, T. P., Miller, J. V., Powers, D. B., Burnier, J. P., Ng, P. G. & Wells, J. A. (1986). Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering. Science, 233, 659-663.
-
(1986)
Science
, vol.233
, pp. 659-663
-
-
Estell, D.A.1
Graycar, T.P.2
Miller, J.V.3
Powers, D.B.4
Burnier, J.P.5
Ng, P.G.6
Wells, J.A.7
-
9
-
-
0023657031
-
Dissection of the structure and activity of the tyrosyl-tRNA synthetase by site-directed mutagenesis
-
Fersht, A. R. (1987). Dissection of the structure and activity of the tyrosyl-tRNA synthetase by site-directed mutagenesis. Biochemistry, 26, 8031-8037.
-
(1987)
Biochemistry
, vol.26
, pp. 8031-8037
-
-
Fersht, A.R.1
-
10
-
-
0021881957
-
Refined structure of α-lytic protease at 1.7 Å resolution
-
Fujinaga, M., Delbaere, L. T. J., Brayer, G. D. & James, M. N. G. (1985). Refined structure of α-lytic protease at 1.7 Å resolution. J. Mol. Biol. 183, 479-502.
-
(1985)
J. Mol. Biol.
, vol.183
, pp. 479-502
-
-
Fujinaga, M.1
Delbaere, L.T.J.2
Brayer, G.D.3
James, M.N.G.4
-
11
-
-
0023176519
-
Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin
-
Graf, L., Craik, C. S., Patthy A., Roczniak, S., Fletterick, R. J. & Rutter, W. J. (1987). Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin. Biochemistry, 26, 2616-2623.
-
(1987)
Biochemistry
, vol.26
, pp. 2616-2623
-
-
Graf, L.1
Craik, C.S.2
Patthy, A.3
Roczniak, S.4
Fletterick, R.J.5
Rutter, W.J.6
-
12
-
-
0024043994
-
Electrostatic complementarity within the substrate-binding pocket of trypsin
-
Graf, L., Jancso, A., Szilagyi, L., Hegyi, G., Pinter, K., Naray-Szabo, G., Hepp, J., Medzihradszky, K. & Rutter, W. J. (1988). Electrostatic complementarity within the substrate-binding pocket of trypsin. Proc. Natl Acad. Sci. USA, 85, 4961-4965.
-
(1988)
Proc. Natl Acad. Sci. USA
, vol.85
, pp. 4961-4965
-
-
Graf, L.1
Jancso, A.2
Szilagyi, L.3
Hegyi, G.4
Pinter, K.5
Naray-Szabo, G.6
Hepp, J.7
Medzihradszky, K.8
Rutter, W.J.9
-
13
-
-
0027245444
-
Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities
-
Graham, L. D., Haggett, K. D., Jennings, P. A., Le Brocque, D. S., Whittaker, R. G. & Schober, P. A. (1993). Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities. Biochemistry, 32, 6250-6258.
-
(1993)
Biochemistry
, vol.32
, pp. 6250-6258
-
-
Graham, L.D.1
Haggett, K.D.2
Jennings, P.A.3
Le Brocque, D.S.4
Whittaker, R.G.5
Schober, P.A.6
-
14
-
-
0023393810
-
Characterization and structure of genes for proteases A and B from Streptomyces griseus
-
Henderson, G., Krygsman, P., Liu, C. J., Davey, C. C. & Malek, L. T. (1987). Characterization and structure of genes for proteases A and B from Streptomyces griseus. J. Bacteriol. 169, 3778-3784.
-
(1987)
J. Bacteriol.
, vol.169
, pp. 3778-3784
-
-
Henderson, G.1
Krygsman, P.2
Liu, C.J.3
Davey, C.C.4
Malek, L.T.5
-
15
-
-
0017844356
-
Amino acid sequence alignment of bacterial and mammalian pancreatic serine proteases based on topological equivalences
-
James, M. N. G., Delbaere, L. T. J. & Brayer, G. D. (1978). Amino acid sequence alignment of bacterial and mammalian pancreatic serine proteases based on topological equivalences. Can. J. Biochem. 56, 396-402.
-
(1978)
Can. J. Biochem.
, vol.56
, pp. 396-402
-
-
James, M.N.G.1
Delbaere, L.T.J.2
Brayer, G.D.3
-
16
-
-
0021193861
-
Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases
-
Kawamura, F. & Doi, R. H. (1984). Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J. Bacteriol. 160, 442-444.
-
(1984)
J. Bacteriol.
, vol.160
, pp. 442-444
-
-
Kawamura, F.1
Doi, R.H.2
-
17
-
-
0040519404
-
Plasmid DNA isolation
-
Plenum Press, New York and London
-
Lovett, P. & Amulos, N. P. J. (1989). Plasmid DNA isolation. In Bacillus, pp. 355, Plenum Press, New York and London.
-
(1989)
Bacillus
, pp. 355
-
-
Lovett, P.1
Amulos, N.P.J.2
-
18
-
-
71849104860
-
Protein measurement with the Folin phenol reagent
-
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
-
(1951)
J. Biol. Chem.
, vol.193
, pp. 265-275
-
-
Lowry, O.H.1
Rosebrough, N.J.2
Farr, A.L.3
Randall, R.J.4
-
19
-
-
0003785155
-
-
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Miller, J. H. (1972). Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
-
(1972)
Experiments in Molecular Genetics
-
-
Miller, J.H.1
-
20
-
-
0027486963
-
A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding
-
Nienaber, V. L., Breddam, K. & Birktoft, J. J. (1993). A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding. Biochemistry, 32, 11469-11475.
-
(1993)
Biochemistry
, vol.32
, pp. 11469-11475
-
-
Nienaber, V.L.1
Breddam, K.2
Birktoft, J.J.3
-
21
-
-
0000295996
-
Secretion and autoproteolytic maturation of subtilisin
-
Power, S. D., Adams, R. M. & Wells, J. A. (1986). Secretion and autoproteolytic maturation of subtilisin. Proc. Natl Acad. Sci. USA, 83, 3096-3100.
-
(1986)
Proc. Natl Acad. Sci. USA
, vol.83
, pp. 3096-3100
-
-
Power, S.D.1
Adams, R.M.2
Wells, J.A.3
-
23
-
-
0023881841
-
Refined crystal structure of Streptomyces griseus trypsin at 1.7 Å resolution
-
Read, R. J. & James, M. N. (1988). Refined crystal structure of Streptomyces griseus trypsin at 1.7 Å resolution. J. Mol. Biol. 200, 523-551.
-
(1988)
J. Mol. Biol.
, vol.200
, pp. 523-551
-
-
Read, R.J.1
James, M.N.2
-
24
-
-
0021102433
-
Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8 Å resolution
-
Read, R. J., Fujinaga, M., Sielecki, A. R. & James, M. N. G. (1983). Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8 Å resolution. Biochemistry, 22, 4420-4433.
-
(1983)
Biochemistry
, vol.22
, pp. 4420-4433
-
-
Read, R.J.1
Fujinaga, M.2
Sielecki, A.R.3
James, M.N.G.4
-
25
-
-
0003903345
-
-
198. (N. Irwin) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Sambrook, J., Fritsch, E. F. & Maniatis, T. (198). Molecular Cloning: a Laboratory Manual (N. Irwin) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
-
Molecular Cloning: A Laboratory Manual
-
-
Sambrook, J.1
Fritsch, E.F.2
Maniatis, T.3
-
26
-
-
0014211618
-
On the size of the active site in proteases. I. Papain
-
Schechter, I. & Berger, A. (1968). On the size of the active site in proteases. I. Papain. Biochem. Biophys. Res. Commun. 27, 157-162.
-
(1968)
Biochem. Biophys. Res. Commun.
, vol.27
, pp. 157-162
-
-
Schechter, I.1
Berger, A.2
-
27
-
-
0027658084
-
Characterization of the gene encoding the glutamic acid-specific protease of Streptomyces griseus
-
Sidhu, S. S., Kalmar, G. B. & Borgford, T. J. (1993). Characterization of the gene encoding the glutamic acid-specific protease of Streptomyces griseus. Biochem. Cell Biol. 71, 454-461.
-
(1993)
Biochem. Cell Biol.
, vol.71
, pp. 454-461
-
-
Sidhu, S.S.1
Kalmar, G.B.2
Borgford, T.J.3
-
28
-
-
0028022483
-
Streptomyces griseus protease C: A novel enzyme of the chymotrypsin superfamily
-
Sidhu, S. S., Kalmar,. B., Willis, L. & Borgford, T. J. (1994). Streptomyces griseus protease C: a novel enzyme of the chymotrypsin superfamily. J. Biol. Chem. 269, 20167-20171.
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 20167-20171
-
-
Sidhu, S.S.1
Kalmar, B.2
Willis, L.3
Borgford, T.J.4
-
29
-
-
0028987143
-
Protease evolution in Streptomyces griseus: Discovery of a novel dimeric enzyme
-
Sidhu, S. S., Kalmar, G. B., Willis, L. & Borgford, T. J. (1995). Protease evolution in Streptomyces griseus: discovery of a novel dimeric enzyme. J. Biol. Chem. 270, 7594-7600.
-
(1995)
J. Biol. Chem.
, vol.270
, pp. 7594-7600
-
-
Sidhu, S.S.1
Kalmar, G.B.2
Willis, L.3
Borgford, T.J.4
-
30
-
-
0024580976
-
Analysis of prepro-α-lytic protease expression in Escherichia coli reveals that the pro region is required for activity
-
Silen, J. L., Frank, D., Fujishige, A., Bone, R. & Agard, D. A. (1989). Analysis of prepro-α-lytic protease expression in Escherichia coli reveals that the pro region is required for activity. J. Bacteriol. 171, 1320-1325.
-
(1989)
J. Bacteriol.
, vol.171
, pp. 1320-1325
-
-
Silen, J.L.1
Frank, D.2
Fujishige, A.3
Bone, R.4
Agard, D.A.5
-
31
-
-
0023046815
-
A new method for predicting signal sequence cleavage sites
-
von Heijne, G. (1986). A new method for predicting signal sequence cleavage sites. Nucl. Acids Res. 14, 4683-4690.
-
(1986)
Nucl. Acids Res.
, vol.14
, pp. 4683-4690
-
-
Von Heijne, G.1
-
32
-
-
0021112782
-
Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis
-
Wells, J. A., Ferrari, E., Henner, D. J., Estell, D. A. & Chen, E. Y. (1983). Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucl. Acids Res. 11, 7911-7925.
-
(1983)
Nucl. Acids Res.
, vol.11
, pp. 7911-7925
-
-
Wells, J.A.1
Ferrari, E.2
Henner, D.J.3
Estell, D.A.4
Chen, E.Y.5
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