A novel vector element providing multicopy vector integration in Arxula adeninivorans

FEMS Yeast Research

Volumn 7, Issue 7, 2007, Pages 1197-1205

  Steinborn, Gerhard ^a   Gellissen, Gerd ^b   Kunze, Gotthard ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b PharmedArtis GmbH   (Germany)

Author keywords

Arxula adeninivorans; Auxotrophic selection marker; Heterologous gene expression; Multicopy integration; Transformation; Yeast integration expression cassette

Indexed keywords

AMYLASE; DNA;

ALEU2 GENE; ARXULA ADENINIVORANS; ATRP1 GENE; CONFERENCE PAPER; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; GENE EXPRESSION; GENE VECTOR; MITOSIS; NONHUMAN; PROMOTER REGION; YEAST;

ALDOSE-KETOSE ISOMERASES; ALPHA-AMYLASES; BACILLUS CEREUS; FUNGAL PROTEINS; GENE DOSAGE; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; MOLECULAR BIOLOGY; PROMOTER REGIONS, GENETIC; RECOMBINATION, GENETIC; SACCHAROMYCETALES;

ARXULA ADENINIVORANS; BACILLUS AMYLOLIQUEFACIENS;

EID: 34548577765     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2007.00280.x     Document Type: Conference Paper

References (31)

1. Agaphonov M, Trushkina PM, Sohn JS, Choi ES, Rhee SK Ter-Avanesyan MD (1999) Vectors for rapid selection of integrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL-1. Yeast 15: 541 551.
2. Böer E, Wartmann T, Luther B, Manteuffel R, Bode R, Gellissen G Kunze G (2004) Characterization of the AINV gene and the encoded invertase from the dimorphic yeast Arxula adeninivorans. Antonie van Leeuwenhoek 86: 121 134.
3. Böer E, Gellissen G Kunze G (2005a) Arxula adeninivorans. Production of Recombinant Proteins (Gellissen G, ed), pp. 89 110. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
4. Böer E, Wartmann T, Schmidt S, Bode R, Gellissen G Kunze G (2005b) Characterization of the AXDH gene and the encoded xylitol dehydrogenase from the dimorphic yeast Arxula adeninivorans. Antonie van Leeuwenhoek 87: 233 243.
5. Böer E, Steinborn G, Matros A, Mock HP, Gellissen G Kunze G (2007) Production of interleukin-6 in Arxula adeninivorans, Hansenula polymorpha and Saccharomyces cerevisiae applying the wide range yeast vector (comed™) system to simultaneous comparative assessment. FEMS Yeast Res (in press).
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248 254.
7. Büttner R, Schubert U, Bode R Birnbaum D (1990) Purification and partial characterization of external and internal invertases from two strains of Trichosporon adeninivorans. Acta Biotechnol 10: 361 370.
8. Erhart E Hollenberg CP (1983) The presence of a defect LEU2 gene on 2 μ DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number. J Bacteriol 156: 625 635.
9. Gatzke R, Weydemann U, Janowicz ZA Hollenberg CP (1995) Stable multicopy integration of vector sequences in Hansenula polymorpha. Appl Microbiol Biotechnol 43: 844 849.
10. Gellissen G, Kunze G, Gaillardin C, Cregg JM, Berardi E, Veenhuis M van der Klei I (2005) New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - a comparison. FEMS Yeast Res 5: 1079 1096.
11. Hanahan D (1983) Studies on transformation of E. coli with plasmids. J Mol Biol 166: 557 580.
12. Ilgen C, Lin-Cereghino J Cregg JM (2005) Pichia pastoris. Production of Recombinant Proteins. Novel Microbial and Eukaryotic Expression systems (Gellissen G, ed), pp. 143 162. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
13. Juretzek T, LeDall MT, Mauersberger S, Gaillardin C, Barth G Nicaud JM (2001) Vectors of gene expression and amplification in the yeast Yarrowia lipolytica. Yeast 18: 97 113.
14. Kaur P, Lingner A, Singh B, Böer E, Polajeva J, Steinborn G, Bode R, Gellissen G, Satyanarayana T Kunze G (2007) Characterization of the APHO1 gene encoding an extracellular acid phosphatase with broad substrate specificity from the yeast Arxula adeninivorans. Antonie van Leeuwenhoek 91: 45 55.
15. Klabunde J, Driesel A, Waschk D, Gellissen G, Hollenberg CP Suckow M (2002) Single-step co-integration of multiple expressible heterologous genes into the ribosomal DNA of the methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol 58: 797 805.
16. Kunze G Kunze I (1994) Characterization of Arxula adeninivorans strains from different habitats. Antonie van Leeuwenhoek 65: 607 614.
17. Kunze I Kunze G (1996) Arxula adeninivorans. Nonconventional yeasts (Wolf K, ed), pp. 389 409. Springer Verlag, Berlin-Heidelberg.
18. Le Dall MT, Nicaud JM Gaillardin C (1994) Multiple-copy integration in the yeast Yarrowia lipolytica. Curr Genet 26: 38 44.
19. Lopes TS, Klootwijk J, Veenstra AE, van der Aar PC, van Heerikhuizen H, Raue HA Planta RJ (1989) High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression. Gene 79: 199 206.
20. Lopes TS, de Wijs IJ, Steenhauer SI, Verbakel J Planta RJ (1996) Factors affecting the mitotic stability of high-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae. Yeast 12: 467 477.
21. Nieto A, Prieto JA Sanz P (1999) Stable high-copy-number integration of Aspergillus oryzae alpha-amylase cDNA in an industrial baker's yeast strain. Biotechnol Prog 15: 459 466.
22. Rose MD, Winston F Hieter P (1990) Methods in yeast genetics. A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.
23. Rösel H Kunze G (1998) Integrative transformation of the dimorphic yeast Arxula adeninivorans LS3 based on Hygromycin B resistance. Curr Genet 33: 157 163.
24. Steinborn G, Gellissen G Kunze G (2005) Assessment of Hansenula polymorpha and Arxula adeninivorans-derived rDNA-targeting elements for the design of Arxula adeninivorans expression vectors. FEMS Yeast Res 5: 1047 1054.
25. Steinborn G, Böer E, Scholz A, Tag K, Kunze G Gellissen G (2006) Application of a wide-range yeast vector (comed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts. Microbial Cell Factories 5: 33.
26. Steinborn G, Wartmann T, Gellissen G Kunze G (2007) Construction of an Arxula adeninivorans host-vector system based on trp1 complementation. J Biotechnol 127: 392 401.
27. Tanaka A, Ohnishi N Fukui S (1967) Studies on the formation of vitamins and their function in hydrocarbon fermentation. Production of vitamin B6 by Candida albicans in hydrocarbon medium. J Ferment Technol 45: 617 623.
28. Terentiev Y, Breuer U, Babel W Kunze G (2004) Non-conventional yeasts as producers of polyhydroxyalkanoates - genetic engineering of Arxula adeninivorans. Appl Microbiol Biotechnol 64: 376 381.
29. Wartmann T, Rösel H, Kunze I, Bode R Kunze G (1998) AILV1 gene from the yeast Arxula adeninivorans LS3 - a new selective transformation marker. Yeast 14: 1017 1025.
30. Wartmann T, Böer E, Huarto-Pico A, Sieber H, Bartelsen O, Gellissen G Kunze G (2002) High-level production and secretion of recombinant proteins by the dimorphic yeast Arxula adeninivorans. FEMS Yeast Res 2: 363 369.
31. Wartmann T, Stoltenburg R, Böer E, Sieber H, Bartelsen O, Gellissen G Kunze G (2003) The ALEU2 gene - a new component for an Arxula adeninivorans-based expression platform. FEMS Yeast Res 3: 223 232.