MEK/ERK signaling controls osmoregulation of nucleus pulposus cells of the intervertebral disc by transactivation of TonEBP/OREBP

Journal of Bone and Mineral Research

Volumn 22, Issue 7, 2007, Pages 965-974

  Tsai, Tsung Ting ^a,b   Guttapalli, Asha ^a   Agrawal, Amit ^a   Albert, Todd J ^a   Shapiro, Irving M ^a   Risbud, Makarand V ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b CHANG GUNG UNIVERSITY   (Taiwan)

Author keywords

Cell adaptation; Extracellular signal regulated kinase signaling; Intervertebral disc; Nucleus pulposus; Osmotic pressure; TonEBP

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 6 (4 FLUOROPHENYL) 2,3 DIHYDRO 5 (4 PYRIDYL)IMIDAZO[2,1 B]THIAZOLE; BINDING PROTEIN; HEAT SHOCK PROTEIN 70; HYPERTONIC SOLUTION; LUCIFERASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE P38; SMALL INTERFERING RNA; TAURINE TRANSPORTER; TONICITY ENHANCER BINDING PROTEIN; UNCLASSIFIED DRUG; CARRIER PROTEIN; MEMBRANE PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; NFAT5 PROTEIN, RAT; TRANSCRIPTION FACTOR;

ADAPTATION; ANIMAL CELL; ARTICLE; AUTOREGULATION; CELL CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; DOWN REGULATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; GEL MOBILITY SHIFT ASSAY; HELA CELL; HUMAN; HUMAN CELL; HYPEROSMOTIC STRESS; NONHUMAN; NUCLEUS PULPOSUS; NUCLEUS PULPOSUS CELL; OSMOREGULATION; PLASMID; PROMOTER REGION; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; RAT; SIGNAL TRANSDUCTION; ANIMAL; CYTOLOGY; ELECTROLYTE BALANCE; ENZYMOLOGY; GENETICS; INTERVERTEBRAL DISK; METABOLISM; REPORTER GENE; TRANSACTIVATION;

ANIMALS; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GENES, REPORTER; HELA CELLS; HUMANS; HYPERTONIC SOLUTIONS; INTERVERTEBRAL DISK; MAP KINASE SIGNALING SYSTEM; MEMBRANE GLYCOPROTEINS; MEMBRANE TRANSPORT PROTEINS; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; RATS; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; WATER-ELECTROLYTE BALANCE;

EID: 34548546827     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/jbmr.070322     Document Type: Article

References (34)

1. 1 Maroudas A, Muir H, Wingham J 1969 The correlation of fixed negative charge with glycosaminoglycan content of human articular cartilage. Biochim Biophys Acta 177: 492–500.
2. 2 Maroudas A 1970 Distribution and diffusion of solutes in articular cartilage. Biophys J 10: 365–379.
3. 3 Urban JP, Holm S, Maroudas A 1978 Diffusion of small solutes into the intervertebral disc: As in vivo study. Biorheology 15: 203–221.
4. 4 Kraemer J, Kolditz D, Gowin R 1985 Water and electrolyte content of human intervertebral discs under variable load. Spine 10: 69–71.
5. 5 Tsai TT, Danielson KG, Guttapalli A, Oguz E, Albert TJ, Shapiro IM, Risbud MV 2006 TonEBP/OREBP is a regulator of nucleus pulposus cell function and survival in the intervertebral disc. J Biol Chem 281: 25416–25424.
6. 6 Miyakawa H, Woo SK, Dahl SC, Handler JS, Kwon HM 1999 Tonicity‐responsive enhancer binding protein, a rel‐like protein that stimulates transcription in response to hypertonicity. Proc Natl Acad Sci USA 96: 2538–2542.
7. 7 Lopez‐Rodriguez C, Aramburu J, Rakeman AS, Rao A 1999 NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. Proc Natl Acad Sci USA 96: 7214–7219.
8. 8 Alexandre H, Ansanay‐Galeote V, Dequin S, Blondin B 2001 Global gene expression during short‐term ethanol stress in Saccharomyces cerevisiae. FEBS Lett 498: 98–103.
9. 9 Ansell R, Granath K, Hohmann S, Thevelein JM, Adler L 1997 The two isoenzymes for yeast NAD‐dependent glycerol 3‐phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16: 2179–2187.
10. 10 Posas F, Saito H 1997 Osmotic activation of the HOG MAP kinase pathway via Ste11p MAPKKK: Scaffold role of Pbs2p MAPKK. Science 276: 1702–1705.
11. 11 Zhang Z, Ferraris JD, Brooks HL, Brisc I, Burg MB 2003 Expression of osmotic stress‐related genes in tissues of normal and hyposmotic rats. Am J Physiol Renal Physiol 285: F688–F693.
12. 12 Ito T, Fujio Y, Hirata M, Takatani T, Matsuda T, Muraoka S, Takahashi K, Azuma J 2004 Expression of taurine transporter is regulated through the TonE (tonicity‐responsive element)/TonEBP (TonE‐binding protein) pathway and contributes to cytoprotection in HepG2 cells. Biochem J 382: 177–182.
13. 13 Ko BC, Ruepp B, Bohren KM, Gabbay KH, Chung SS 1997 Identification and characterization of multiple osmotic response sequences in the human aldose reductase gene. J Biol Chem 272: 16431–16437.
14. 14 Miyakawa H, Woo SK, Chen CP, Dahl SC, Handler JS, Kwon HM 1998 Cis‐ and trans‐acting factors regulating transcription of the BGT1 gene in response to hypertonicity. Am J Physiol 274: F753–F761.
15. 15 Rim JS, Atta MG, Dahl SC, Berry GT, Handler JS, Kwon HM 1998 Transcription of the sodium/myo‐inositol cotransporter gene is regulated by multiple tonicity‐responsive enhancers spread over 50 kilobase pairs in the 5′‐flanking region. J Biol Chem 273: 20615–20621.
16. 16 Woo SK, Lee SD, Na KY, Park WK, Kwon HM 2002 TonEBP/NFAT5 stimulates transcription of HSP70 in response to hypertonicity. Mol Cell Biol 22: 5753–5760.
17. 17 Shim EH, Kim JI, Bang ES, Heo JS, Lee JS, Kim EY, Lee JE, Park WY, Kim SH, Kim HS, Smithies O, Jang JJ, Jin DI, Seo JS 2002 Targeted disruption of hsp70.1 sensitizes to osmotic stress. EMBO Rep 3: 857–861.
18. 18 Ko BC, Turck CW, Lee KW, Yang Y, Chung SS 2000 Purification, identification, and characterization of an osmotic response element binding protein. Biochem Biophys Res Commun 270: 52–61.
19. 19 Dahl SC, Handler JS, Kwon HM 2001 Hypertonicity‐induced phosphorylation and nuclear localization of the transcription factor TonEBP. Am J Physiol 280: C248–C253.
20. 20 Cai Q, Ferraris JD, Burg MB 2005 High NaCl increases TonEBP/OREBP mRNA and protein by stabilizing its mRNA. Am J Physiol Renal Physiol 289: F803–F807.
21. 21 Ferraris JD, Williams CK, Persaud P, Zhang Z, Chen Y, Burg MB 2002 Activity of the TonEBP/OREBP transactivation domain varies directly with extracellular NaCl concentration. Proc Natl Acad Sci USA 99: 739–744.
22. 22 Ko BC, Lam AK, Kapus A, Fan L, Chung SK, Chung SS 2002 Fyn and p38 signaling are both required for maximal hypertonic activation of the osmotic response element‐binding protein/tonicity‐responsive enhancer‐binding protein (OREBP/TonEBP). J Biol Chem 277: 46085–46092.
23. 23 Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB 2002 cAMP‐independent role of PKA in tonicity‐induced transactivation of tonicity‐responsive enhancer/ osmotic response element‐binding protein. Proc Natl Acad Sci USA 99: 16800–16805.
24. 24 Risbud MV, Di Martino A, Guttapalli A, Seghatoleslami R, Denaro V, Vaccaro AR, Albert TJ, Shapiro IM 2006 Toward an optimum system for intervertebral disc organ culture: TGF‐beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF‐beta‐R expression and ERK signaling. Spine 31: 884–890.
25. 25 Nahm O, Woo SK, Handler JS, Kwon HM 2002 Involvement of multiple kinase pathways in stimulation of gene transcription by hypertonicity. Am J Physiol Cell Physiol 282: C49–C58.
26. 26 Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM 2006 Nucleus pulposus cells express HIF‐1alpha under normoxic culture conditions: A metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem 98: 152–159.
27. 27 Dignam JD, Lebovitz RM, Roeder RG 1983 Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11: 1475–1489.
28. 28 Vanhoutte P, Barnier JV, Guibert B, Pages C, Besson MJ, Hipskind RA, Caboche J 1999 Glutamate induces phosphorylation of Elk‐1 and CREB, along with c‐fos activation, via an extracellular signal‐regulated kinase‐dependent pathway in brain slices. Mol Cell Biol 19: 136–146.
29. 29 Robinson MJ, Harkins PC, Zhang J, Baer R, Haycock JW, Cobb MH, Goldsmith EJ 1996 Mutation of position 52 in ERK2 creates a nonproductive binding mode for adenosine 5′‐triphosphate. Biochemistry 35: 5641–5646.
30. 30 Vaidya RJ, Ray RM, Johnson LR 2005 MEK1 restores migration of polyamine‐depleted cells by retention and activation of Rac1 in the cytoplasm. Am J Physiol Cell Physiol 288: C350–C359.
31. 31 Ge B, Gram H, Di Padova F, Huang B, New L, Ulevitch RJ, Luo Y, Han J 2002 MAPKK‐independent activation of p38alpha mediated by TAB1‐dependent autophosphorylation of p38alpha. Science 295: 1291–1294.
32. 32 Risbud MV, Fertala J, Vresilovic EJ, Albert TJ, Shapiro IM 2005 Nucleus pulposus cells upregulate PI3K/Akt and MEK/ERK signaling pathways under hypoxic conditions and resist apoptosis induced by serum withdrawal. Spine 30: 882–889.
33. 33 Kojima R, Randall JD, Ito E, Manshio H, Suzuki Y, Gullans SR 2004 Regulation of expression of the stress response gene, Osp94: Identification of the tonicity response element and intracellular signalling pathways. Biochem J 380: 783–794.
34. 34 Murphy LO, Blenis J 2006 MAPK signal specificity: The right place at the right time. Trends Biochem Sci 31: 268–275.